Are Human and Mouse Satellite Cells Really the Same?

Boldrin, Luisa; Muntoni, Francesco; Morgan, Jennifer E.

doi:10.1369/jhc.2010.956201

Cited by 114 publications

(88 citation statements)

References 225 publications

(301 reference statements)

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“…The proliferative ability and temporal expression of mRNA by satellite cells seems to be dependent on the host and the muscle from which they are collected (Powell et al, 2014). Additional levels of heterogeneity are known to exist between satellite cells populations, as well as different extracellular matrix protein compositions, growth kinetics, myoblast fusion capacities, transcription factor profiles, motility kinetics and migratory properties (Biressi and Rando, 2010;Boldrin et al, 2010;Li et al, 2011;Li and Johnson, 2013). There are increasing evidence through gene expression profiling and cell-surface marker analysis that satellite cells are a heterogeneous population (Motohashi and Asakura, 2014).…”

Section: Discussionmentioning

confidence: 99%

Temporal correlation between differentiation factor expression and microRNAs in Holstein bovine skeletal muscle

Miretti

Volpe

Martignani

et al. 2017

Animal

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Satellite cells are adult stem cells located between the basal lamina and sarcolemma of muscle fibers. Under physiological conditions, satellite cells are quiescent, but they maintain a strong proliferative potential and propensity to differentiate, which underlies their critical role in muscle preservation and growth. MicroRNAs (miRNAs) play essential roles during animal development as well as in stem cell self-renewal and differentiation regulation. MiRNA-1, miRNA-133a and miRNA-206 are closely related muscle-specific miRNAs, and are thus defined myomiRNAs. MyomiRNAs are integrated into myogenic regulatory networks. Their expression is under the transcriptional and post-transcriptional control of myogenic factors and, in turn, they exhibit widespread control of muscle gene expression. Very little information is available about the regulation and behavior of satellite cells in large farm animals, in particular during satellite cell differentiation. Here, we study bovine satellite cells (BoSCs) undergoing a differentiation process and report the expression pattern of selected genes and miRNAs involved. Muscle samples of longissimus thoracis from Holstein adult male animals were selected for the collection of satellite cells. All satellite cell preparations demonstrated myotube differentiation. To characterize the dynamics of several transcription factors expressed in BoSCs, we performed real-time PCR on complementary DNA generated from the total RNA extracted from BoSCs cultivated in growth medium (GM) or in differentiation medium (DM) for 4 days. In the GM condition, BoSCs expressed the satellite cell lineage markers as well as transcripts for the myogenic regulatory factors. At the time of isolation from muscle, PAX7 was expressed in nearly 100% of BoSCs; however, its messenger RNA (mRNA) levels dramatically decreased between 3 and 6 days post isolation ( P < 0.01). MyoD mRNA levels increased during the 1st day of cultivation in DM (day 7; P < 0.02), showing a gradual activation of the myogenic gene program. During the subsequent 4 days of culture in DM, several tested genes, including MRF4, MYOG, MEF2C, TMEM8C, DES and MYH1, showed increased expression ( P < 0.05), and these levels remained high throughout the culture period investigated. Meanwhile, the expression of genes involved in the differentiation process also miRNA-1, miRNA-133a and miRNA-206 were strongly up-regulated on the 1st day in DM (day 7; P < 0.05). Analysis revealed highly significant correlations between myomiRNAs expression and MEF2C, MRF4, TMEM8C, DES and MYH1 gene expression ( P < 0.001). Knowledge about the transcriptional changes correlating with the growth and differentiation of skeletal muscle fibers could be helpful for developing strategies to improve production performance in livestock.

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Section: Discussionmentioning

confidence: 99%

Temporal correlation between differentiation factor expression and microRNAs in Holstein bovine skeletal muscle

Miretti

Volpe

Martignani

et al. 2017

Animal

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“…This helps to enrich the myoblast fraction as myoblasts show a higher affinity for collagen IV/laminin-coated surfaces than for collagen I/fibronectin-coated surfaces, the latter is preferred by fibroblasts (Kuhl et al, 1986). NCAM was the first marker used to identify human muscle satellite cells, and this marker is expressed in satellite cells, myoblasts, myotubes and muscle fibers during development (Boldrin et al, 2010). This marker is expressed in proliferating human and rat muscle cells, but is not a reliable marker in mice muscle cells; mice muscle cells express NCAM only when they have become committed to differentiation (Capkovic et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, differences are also observed between primary cell model systems from different species. The neural cell adhesion marker (NCAM) is expressed in proliferating human and rat cells (Boldrin et al, 2010), but not in mice muscle cells (Capkovic et al, 2008). Cattle are evolutionary closer to humans than rodents, and the entire bovine genome is fully sequenced in order to provide an important supplement for human medical research (Tellam et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

et al. 2013

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Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation and differentiation features, were characterized using immunocytochemistry, western blotting and real-time PCR. We observed a reorganization of the Golgi complex in differentiated cells. The Golgi complex transformed to a highly fragmented network of small stacks of cisternae positioned throughout the myotubes as well as around the nucleus. Different extracellular matrix (ECM) components were used as surface coatings in order to improve cell culture conditions. Our experiments demonstrated improved proliferation and early differentiation for cells grown on surface coatings containing a mixture of both glycosaminoglycans (GAGs) and fibrous proteins. We suggest that GAGs and fibrous proteins mixed together into a composite biomaterial can mimic a natural ECM, and this could improve myogenesis for in vitroin vitro cell cultures.Abbreviations: used: ECMextracellular (The author would like to point out that there should be a space between the abbrevations and the subsequent explanation. ) matrix; GAGsglucosaminoglycans;MRFGAGsglucosaminoglycans, MRF, myogenic regulatory transcription factor; PGproteoglycan

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“…The most reliable markers of human satellite cells are PAX7 and CD56 (Boldrin et al 2010), and MyoD expression in satellite cells has been previously reported to depend on myogenic differentiation (Bareja et al 2014). Myogenic differentiation was induced by replacing the growth medium with DMEM/Ham's F10 mixture supplemented with 5% horse serum and 1% penicillin-streptomycin (Asakura et al 2002).…”

Section: Immunofluorescence Analysismentioning

confidence: 99%

“…Muscle satellite cells are typical muscle stem cells that exhibit self-renewal and myogenic differentiation capacities (Judson et al 2013;Gigliotti et al 2016) and are characterized by expression of cluster of differentiation 56 (CD56) in humans (Boldrin et al 2010). These generally quiescent cells are located between the basal lamina and sarcolemma of the skeletal muscle fibers (Zammit et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration

Koide

Hagiwara

Tsuchiya

et al. 2018

Tohoku J. Exp. Med.

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Rotator cuff tears (RCTs) are a common shoulder problem in the elderly that can lead to both muscle atrophy and fatty infiltration due to less physical load. Satellite cells, quiescent cells under the basal lamina of skeletal muscle fibers, play a major role in muscle regeneration. However, the myogenic potency of human satellite cells in muscles with fatty infiltration is unclear due to the difficulty in isolating from small samples, and the mechanism of the progression of fatty infiltration has not been elucidated. The purpose of this study was to analyze the population of myogenic and adipogenic cells in disused supraspinatus (SSP) and intact subscapularis (SSC) muscles of the RCTs from the same patients using fluorescenceactivated cell sorting. The microstructure of the muscle with fatty infiltration was observed as a whole mount condition under multi-photon microscopy. Myogenic differentiation potential and gene expression were evaluated in satellite cells. The results showed that the SSP muscle with greater fatty infiltration surrounded by collagen fibers compared with the SSC muscle under multi-photon microscopy. A positive correlation was observed between the ratio of muscle volume to fat volume and the ratio of myogenic precursor to adipogenic precursor. Although no difference was observed in the myogenic potential between the two groups in cell culture, satellite cells in the disused SSP muscle showed higher intrinsic myogenic gene expression than those in the intact SSC muscle. Our results indicate that satellite cells from the disused SSP retain sufficient potential of muscle growth despite the fatty infiltration.

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Are Human and Mouse Satellite Cells Really the Same?

Cited by 114 publications

References 225 publications

Temporal correlation between differentiation factor expression and microRNAs in Holstein bovine skeletal muscle

Temporal correlation between differentiation factor expression and microRNAs in Holstein bovine skeletal muscle

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration

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