Comparison between capillary electrophoresis and HPLC-FL for ochratoxin A quantification in wine

González-Peñas, Elena; Leache, C; Ceráin, Adela López de; Lizarraga, Elena

doi:10.1016/j.foodchem.2005.05.007

Cited by 48 publications

(24 citation statements)

References 11 publications

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“…Techniques based on immunoaffinity columns for clean-up sample are frequently used but they have a high cost. 25,29,30 The validated in-house method showed good linearity. The linear regression coefficient of standard solution curve (y = 5.067x + 1.6786) for OTA within the concentration ranging from 0.8 to 32 μg L -1 was 0.996.…”

Section: Resultsmentioning

confidence: 90%

“…After the first detection of OTA in wine, 24 several surveys were conducted in several countries to examine the true occurrence of this toxin in wine [25][26][27] as well [28][29][30] OTA is usually extracted with acidified organic solvents (e.g acidified chloroform). Clean-up is carried out by liquid-liquid or solid-phase extraction (SPE).…”

Section: Introductionmentioning

confidence: 99%

“…31 Monoclonal antibody based immunoaffinity columns (IACs) have also been developed to substitute the traditional solvent cleanup. [28][29][30] However, the relatively expensive and limited shelf-life of IACs are disadvantageous for use in large survey programs of OTA. Furthermore, because the antibodies used in IACs are fragile proteins, which can readily lose their binding affinity as a result of subtle changes in conditions, such as temperature and pH, experimental and storage conditions need to be carefully controlled and monitored.…”

Section: Introductionmentioning

confidence: 99%

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Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device

Welke¹,

Hoeltz²,

Dottori³

et al. 2010

J. Braz. Chem. Soc.

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Um método foi desenvolvido e validado para a determinação de ocratoxina A (OTA) em vinho. Foi utilizado um sistema contendo um detector de carga acoplada (CCD) para adquirir as imagens fluorescentes da micotoxina obtidas a partir das placas de cromatografia em camada delgada de alta eficiência (HPTLC) sob luz ultravioleta. O método desenvolvido mostrou recuperação média , which is higher than the limit of 2 μg L -1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine.

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Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device

Welke¹,

Hoeltz²,

Dottori³

et al. 2010

J. Braz. Chem. Soc.

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“…González-Peñas et al [101] recently reported a new CE method with diode array detection (DAD) for the analysis of OA in wine. In this work, the effect of various parameters on the separation efficiency and sensitivity was studied.…”

Section: Ochratoxin Amentioning

confidence: 99%

Detection of microbial food contaminants and their products by capillary electromigration techniques

García‐Cañas

Cifuentes

2007

Electrophoresis

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Abbreviations: AFLP, amplified fragment length polymorphism; AGE, agarose gel electrophoresis, ASP, amnesic shellfish poisoning; BGE, background electrolyte; CFP, ciguatera fish poisoning; DA, domoic acid; DCIP, 2,6-dichlorophenolindophenol; DSP, diarrhetic shellfish poisoning; DTX, dinophysistoxin; ERIC, enterobacterial repetitive intergenic consensus; FB1, Fumonisin type 1; GTX, gonyautoxins; MC, microcystins; MLVA, multiple locus variable-number tandem-repeat; MTX, maitotoxin; NEO, neosaxitosin; NSP, neurotoxic shellfish poisoning; OA, ochratoxin A; OkA, okadaic acid; PSP, paralytic shellfish poisoning; PEO, polyethylenoxide; STX, saxitoxin; TMV, tobacco mosaic virus; T-RFLP, terminal restriction fragment length polymorphism; TTX, tetrodotoxin; UTIs, urinary tract infections.

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“…Köller et al [35] used silica gel columns for the sensitive determination of OA in house dust. There are some papers in which IAC have been used prior to CE analysis to determine OA present in wine samples [32,34,36,38]. The use of this type of columns allows the determination of OA in wine samples using CE-UV because the interferences present in the matrix are eliminated and the target analyte preconcentrated.…”

Section: Introductionmentioning

confidence: 99%

Combined use of supported liquid membrane and solid‐phase extraction to enhance selectivity and sensitivity in capillary electrophoresis for the determination of ochratoxin A in wine

2008

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This paper proposes a novel strategy to enhance selectivity and sensitivity in CE, using supported liquid membrane (SLM) and off-line SPE simultaneously. The determination of ochratoxin A (OA) in wine has been used to demonstrate the potential of this methodology. In the SLM step, the donor phase (either a 20 mL volume of a standard solution at pH 1 or a wine sample at pH 8) was placed in a vial, where a micromembrane extraction unit accommodating the acceptor phase (1 mL water, pH 11) in its lumen was immersed. The SLM was constructed by impregnating a porous Fluoropore Teflon (PTFE) membrane with a water-immiscible organic solvent (octanol). In the off-line SPE step, the nonpolar sorbent (C-18, 4 mg) selectively retained the target ochratoxin, enabling small volumes of acceptor phase (1 mL) to be introduced. The captured analytes were eluted in a small volume of methanol (0.1 mL). This procedure resulted in sample cleanup and concentration enhancement. The method was evaluated for accuracy and precision, and its RSD found to be 5%. The LODs for OA in the standard solutions and wine samples were 0.5 and 30 microg/L, respectively. The results obtained demonstrate that SLM combined with off-line is a good alternative to the use of immunoaffinity columns prior to CE analysis.

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Comparison between capillary electrophoresis and HPLC-FL for ochratoxin A quantification in wine

Cited by 48 publications

References 11 publications

Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device

Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device

Detection of microbial food contaminants and their products by capillary electromigration techniques

Combined use of supported liquid membrane and solid‐phase extraction to enhance selectivity and sensitivity in capillary electrophoresis for the determination of ochratoxin A in wine

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