Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device

Abstract

Um método foi desenvolvido e validado para a determinação de ocratoxina A (OTA) em vinho. Foi utilizado um sistema contendo um detector de carga acoplada (CCD) para adquirir as imagens fluorescentes da micotoxina obtidas a partir das placas de cromatografia em camada delgada de alta eficiência (HPTLC) sob luz ultravioleta. O método desenvolvido mostrou recuperação média , which is higher than the limit of 2 μg L -1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine.

Determination of Ochratoxin A in Food by High Performance Liquid Chromatography

“…Alternative detection methods, such as photodiode array detection (LC-DAD) (Soleas, Yan, and Goldberg 2001) or mass spectrometry (LC-MS) and tandem MS (LC-MS=MS) (Zö llner and Mayer-Helm 2006) have also been proposed. Other applied techniques for OTA determination are high performance thin layer chromatography (HPTLC) (Welke et al 2010), gas chromatography coupled with MS (GC-MS) (Olsson et al 2002), enzyme immunoassay (EIA) (Saha et al 2007), and fluorescence polarization immunoassay (Zezza et al 2009). Also, capillary electrophoresis (CE) with UV=Vis detection has also been proposed for the determination of OTA in wine (Almeda, Arce, and Valcárcel 2008;González-Peñ as et al 2006).…”

Exposure to ochratoxin A through consumption of grape juices produced by steam distillation method and intended for school meals

“…OTA was extracted following the developed and validated method by WELKE et al (2010) and samples were analyzed using high performance liquid chromatography (HPLC) with fluorescence detector (Agilent series 1100, USA) that was equipped with an online degasser, a quaternary pump, and an automatic injector ( Figure 1A-C). For fluorescent detection of OTA the excitation wavelength was 333nm, and the emission wavelength was 443nm.…”

Occurrence of ochratoxin A in grapes, juices and wines and risk assessment related to this mycotoxin exposure

“…Occurrence of OTA in wines at levels above 2ng mL -1 (Table 2) was observed in several countries including Brazil (WELKE et al, 2010), China (ZHANG et al, 2013), Spain (BELLI et al, 2004), Greece (SARIGIANNIS et al, 2014), Italy (VISCONTI et al, 1999;BRERA et al, 2008;PRELLE et al, 2013), Portugal (PEN et al, 2010, Russia (RUSANOVA et al, 2009) and Turkey (ALTIOKKA et al, 2009). These results demonstrate that the OTA exposure through wine consumption may be a concern at these countries.…”