Comparison between autologous noncultured epidermal cell suspension and suction blister epidermal grafting in stable vitiligo: a randomized study

Budania, Anil; Parsad, Davinder; Kanwar, Amrinder J.; Dogra, Sunil

doi:10.1111/bjd.12007

Cited by 74 publications

(89 citation statements)

References 17 publications

(22 reference statements)

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“…To date, many studies have used cultured/non-cultured melanocytes with/without keratinocytes or epidermal grafts containing melanocytes as treatment for depigmented lesions. Compared with epidermal grafting, transplantation of a non-cultured epidermal suspension showed better results, suggesting that keratinocytes and niche factors are crucial for repigmentation [102] .…”

Section: Expert Opinion: Potential Clinical Applications Of Melanomentioning

confidence: 99%

Melanocyte stem cells as potential therapeutics in skin disorders

Lee

Fisher

2014

Expert Opinion on Biological Therapy

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Introduction Melanocytes produce pigment granules that color both skin and hair. In the hair follicles melanocytes are derived from stem cells (MelSC) that are present in hair bulges or sub-bulge regions and function as melanocyte reservoirs. Quiescence, maintenance, activation, and proliferation of MelSC are controlled by specific activities in the microenvironment that can influence the differentiation and regeneration of melanocytes. Therefore, understanding MelSC and their niche may lead to use of MelSC in new treatments for various pigmentation disorders. Areas covered We describe here pathophysiological mechanisms by which melanocyte defects lead to skin pigmentation disorders such as vitiligo and hair graying. The development, migration, and proliferation of melanocytes and factors involved in the survival, maintenance, and regeneration of MelSC are reviewed with regard to the biological roles and potential therapeutic applications in skin pigmentation diseases. Expert Opinion MelSC biology and niche factors have been studied mainly in murine experimental models. Human MelSC markers or methods to isolate them are much less well understood. Identification, isolation and culturing of human MelSC would represent a major step toward new biological therapeutic options for patients with recalcitrant pigmentary disorders or hair graying. By modulating the niche factors for MelSC it may one day be possible to control skin pigmentary disorders and prevent or reverse hair graying.

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Section: Expert Opinion: Potential Clinical Applications Of Melanomentioning

confidence: 99%

Melanocyte stem cells as potential therapeutics in skin disorders

Lee

Fisher

2014

Expert Opinion on Biological Therapy

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“…54 In this procedure, a skin fragment is obtained by shaving biopsy and the epidermis is separated from the dermis using a trypsin solution. After several additional steps, a suspension of keratinocytes and melanocytes is obtained and transplanted to the scarified skin in the receiver area.…”

Section: Vitiligo Treatmentmentioning

confidence: 99%

“…After several additional steps, a suspension of keratinocytes and melanocytes is obtained and transplanted to the scarified skin in the receiver area. 39, 48, 54 In a randomized study with 41 patients, repigmentation was considered excellent (90-100% repigmentation) in 71% of lesions in the NCECS group, compared with 27% of lesions on the SBEG technique group. 54 Cell culture has also been used as a means of increasing the number of viable cells and treat even larger areas using less donor tissue.…”

Section: Vitiligo Treatmentmentioning

confidence: 99%

“…39, 48, 54 In a randomized study with 41 patients, repigmentation was considered excellent (90-100% repigmentation) in 71% of lesions in the NCECS group, compared with 27% of lesions on the SBEG technique group. 54 Cell culture has also been used as a means of increasing the number of viable cells and treat even larger areas using less donor tissue. However, this method is costly and requires the support of a tissue culture laboratory.…”

Section: Vitiligo Treatmentmentioning

confidence: 99%

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Vitiligo - Part 2 - classification, histopathology and treatment

Faria

Tarlé

Dellatorre

et al. 2014

An. Bras. Dermatol.

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In an unprecedented effort in the field of vitiligo, a global consensus resulted on a suggested new classification protocol for the disease. The main histopathological finding in vitiligo is the total absence of functioning melanocytes in the lesions, while the inflammatory cells most commonly found on the edges of the lesions are CD4+ and CD8+ T lymphocytes. Physical and pharmacological treatment strategies aim to control the autoimmune damage and stimulate melanocyte migration from the unaffected edges of lesions and the outer hair follicle root sheath to the affected skin; moreover, surgical treatments can be combined with topical and physical treatments.

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“…Non-cultured epidermal cell suspension (NCES) is emerging as the first line of surgical management of stable vitiligo. [10] Major advantages of NCES are that a smaller amount of donor skin is needed to cover large recipient area, little postoperative pain and discomfort, easier placement of cellular graft, excellent color match. Mohanty et al .…”

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confidence: 99%