Comparision of 10% Nitric Acid, Edta and 10% Formic Acid for Tooth Decalcification

Bhat, Nitish; Thakur, Kalpna; Bharadwaj, N. K.; Singh, Ramandeep

doi:10.5530/amdhs.2019.2.8

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…Our study found that the decalcification time of nitric acid was the shortest and EDTA was the longest, in accordance with previous studies. 1 10 11 Because the solubility of calcium phosphate largely depends on solution pH, 12 EDTA with neutral pH takes a longer time compared with acid with a lower pH. Furthermore, EDTA is a chelating agent that gradually captures calcium ions from the superficial surface of apatite crystals and then slowly reduces the size of crystals.…”

Section: Discussionmentioning

confidence: 99%

“…The received blocks were sectioned on microtome for 3 µm slices, and the sections were stained with the hematoxylin and eosin method. 11 The stained section was observed under a light microscope by two examiners blinded to the groups, and histological evaluation of each dental tissue including dentin, cementum and dental pulp tissue was graded from 1 to 3 points score based on the criteria shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Qualitative Histological Evaluation of Various Decalcifying Agents on Human Dental Tissue

et al. 2022

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Objective Dental hard tissue is among the hardest tissue of humans because it contains high amounts of inorganic substances. This leads to difficulty in preparing histological sections for histopathological examination. Acid and chelating agents are generally used to decalcify teeth. We aimed to compare the histological quality of teeth decalcified with various calcifying agents including 5% nitric acid, 50% formic acid with 20% sodium citrate (Anna Morse solution), 10% formic acid, commercial solution, and 14.4% neutral EDTA. Materials and Methods Freshly extracted premolar teeth were fixed and submitted for decalcification using different agents. Histological examination was qualitatively evaluated for tissue integrity and staining quality. Results Dentin integrity of teeth decalcified with all decalcifying agents did not show any statistical differences except that with the formic acid, whereas cementum integrity decalcified with neutral EDTA showed a superior score compared with other agents. Tissue integrity and staining quality of dental pulp cells were the best decalcified with neutral EDTA or Anna Morse solution. Conclusion Our findings demonstrated that EDTA and Anna Morse solution gave a similar efficiency in the preservation of tissue integrity while Anna Morse solution may be recommended as a decalcification agent in routine use due to the more satisfying decalcification time than EDTA.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Qualitative Histological Evaluation of Various Decalcifying Agents on Human Dental Tissue

et al. 2022

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Ice Age apparel – changing prey patterns towards the Last Glacial Maximum and the role of reindeer fur for clothing at Kammern-Grubgraben

Pasda,

Mayr,

Pfeifer

et al. 2024

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Isotope analyses on bone collagen from hunted animals at the Upper Palaeolithic sites Krems-Hundssteig, Krems-Wachtberg and Langenlois A (33 − 29 ka), and Kammern-Grubgraben (23 ka) indicate a strong reduction of ecological herbivore niches towards the Last Glacial Maximum. This change is accompanied by a clear shift in human prey pattern from a more diverse and balanced spectrum of hunted species in the earlier period to a clear focus on reindeer at Kammern-Grubgraben, particularly on young individuals. Moreover, new analyses of the osteological material show that the site with its massive stone constructions and high amount of lithic and faunal material was occupied exclusively in winter. In this paper, we present new results on the age and sex distribution of reindeer at Kammern-Grubgraben. We argue that winter-hunting of young reindeer, in addition to its role in providing energy-rich nutrition and raw material for organic tools, is also indicative of a focus on obtaining high-quality raw material for clothing. The fur of young reindeer in winter is particularly valuable and convenient for the production of clothing for cold environments. Together with the recovered large number of eyed needles, a tool for tight and regular seams, our findings suggest that the production of clothing and other goods made of fur and skin was an important activity at Kammern-Grubgraben.

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Comparision of 10% Nitric Acid, Edta and 10% Formic Acid for Tooth Decalcification

Cited by 2 publications

References 4 publications

Qualitative Histological Evaluation of Various Decalcifying Agents on Human Dental Tissue

Qualitative Histological Evaluation of Various Decalcifying Agents on Human Dental Tissue

Ice Age apparel – changing prey patterns towards the Last Glacial Maximum and the role of reindeer fur for clothing at Kammern-Grubgraben

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