Comparing the identification of Clostridium spp. by two Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) mass spectrometry platforms to 16S rRNA PCR sequencing as a reference standard: A detailed analysis of age of culture and sample preparation

Rapid Tests for Detection of Main Clostridial Toxins

“…In this review it is evident the lack of a fast method and that does not need sophisticated equipment for a quick diagnosis. The most recent advances in the diagnosis of this infection are based on PCR and MALDI TOFF [240][241][242][243]. Here is an important niche for the development of rapid alpha toxin detection techniques, since although this infection does not have a high frequency, its outcome is often fatal.…”

A Clostridium hathewayi isolate in blood culture of a patient with an acute appendicitis

“…The Matrix-Assisted Laser Desorption Ionization Time-of-Flight Spectrometry Mass Spectrometry (MALDI-TOF MS) offers a rapid, accurate, and cost-effective method of microbial characterization and identification [23]. This technology generates characteristic mass spectroscopy fingerprints that are unique signatures for each microorganism.…”

“…Roy Chean et al [23] showed that the Bruker MS identified 100% of Clostridium spp. isolates, when combining results of all sample preparations (Direct Transfer, Extended Direct Transfer, and Full Formic Extraction) from day-2 cultures.…”

MALDI-TOF Mass Spectrometry for Microorganism Identification

“…MALDI-TOF MS has now been demonstrated to be more accurate than phenotypic identification methods (e.g. RapID 32A) (Barba et al, 2014;Chean et al, 2014;Kierzkowska, Majewska, Kuthan, Sawicka-Grzelak, & Mlynarczyk, 2013).…”

“…The use of lower species-level score thresholds, rather than the 2.0 threshold recommended by the manufacturer, has been shown in a number of studies to improve performance of the MALDI Biotyper system for anaerobic isolates (Hsu & Burnham, 2014;Schmitt et al, 2013). Interestingly, this did not appear to be required for the identification of Clostridium species (Chean et al, 2014). In contrast to the impact of score threshold, identification of anaerobic bacteria by MALDI-TOF MS does not appear to be influenced by culture media, incubation time (up to 96 h) or exposure to oxygen (Hsu & Burnham, 2014;Veloo et al, 2014).…”