MALDI-TOF Mass Spectrometry for Microorganism Identification

Bourassa, Lori; Butler-Wu, Susan M.

doi:10.1016/bs.mim.2015.07.003

Cited by 17 publications

(10 citation statements)

References 222 publications

(351 reference statements)

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“…The m/z ratio of each particle is determined and a mass spectrum is generated, representing both m/z and signal intensity of the detected ions. The mass spectrum generated from a bacterium corresponds to high‐abundance soluble proteins, predominantly ribosomal proteins and other abundant cytosolic proteins, and is unique to a bacterium because protein composition differs between different bacterial genera and species (Andrés‐Barrao et al., ; Bourassa & Butler‐Wu, ). MALDI‐TOF is comparable to 16S rRNA gene sequencing in its ability to differentiate to species level, as phylogenetic dendrograms produced by both methods were identical except for certain outlier strains, which were positioned away from their expected taxonomic position on the tree (Andrés‐Barrao et al., ).…”

Section: Characteristics Of the Aab And Their Identificationmentioning

confidence: 99%

Physiology of Acetic Acid Bacteria and Their Role in Vinegar and Fermented Beverages

Lynch

Zannini

Wilkinson

et al. 2019

Comp Rev Food Sci Food Safe

149

139

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Acetic acid bacteria (AAB) have, for centuries, been important microorganisms in the production of fermented foods and beverages such as vinegar, kombucha, (water) kefir, and lambic beer. Their unique form of metabolism, known as “oxidative” fermentation, mediates the transformation of a variety of substrates into products, which are of importance in the food and beverage industry and beyond; the most well‐known of which is the oxidation of ethanol into acetic acid. Here, a comprehensive review of the physiology of AAB is presented, with particular emphasis on their importance in the production of vinegar and fermented beverages. In addition, particular reference is addressed toward Gluconobacter oxydans due to its biotechnological applications, such as its role in vitamin C production. The production of vinegar and fermented beverages in which AAB play an important role is discussed, followed by an examination of the literature relating to the health benefits associated with consumption of these products. AAB hold great promise for future exploitation, both due to increased consumer demand for traditional fermented beverages such as kombucha, and for the development of new types of products. Further studies on the health benefits related to the consumption of these fermented products and guidelines on assessing the safety of AAB for use as microbial food cultures (starter cultures) are, however, necessary in order to take full advantage of this important group of microorganisms.

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Section: Characteristics Of the Aab And Their Identificationmentioning

confidence: 99%

Physiology of Acetic Acid Bacteria and Their Role in Vinegar and Fermented Beverages

Lynch

Zannini

Wilkinson

et al. 2019

Comp Rev Food Sci Food Safe

149

139

View full text Add to dashboard Cite

show abstract

“…In 2015, FDA clearance was announced for more than 200 species of microorganisms using matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS). Two MALDI spectrometers dedicated to this application are available on the market, MALDI Biotyper® and VITEK® 42,43 . These MALDI‐MS platforms perform by targeting the ribosomal proteins as biomarkers for the identification of clinical bacterial, fungal and yeast isolates (method referred to as MALDI Biotyping 44,45 ).…”

Section: Introductionmentioning

confidence: 99%

“…Two MALDI spectrometers dedicated to this application are available on the market, MALDI Biotyper ® and VITEK ® . 42,43 These MALDI-MS platforms perform by targeting the ribosomal proteins as biomarkers for the identification of clinical bacterial, fungal and yeast isolates (method referred to as MALDI Biotyping 44,45 ). Proteins can be analyzed by MS; ionization of the proteins leads to measurement of the mass-to-charge (m/z) ratio, with the great benefit that the analysis is unbiased and a priori knowledge of the proteins is thus not…”

Section: Introductionmentioning

confidence: 99%

Matrix‐assisted laser desorption/ionization mass spectrometry biotyping, an approach for deciphering and assessing the identity of the honeybee pathogen Nosema

Houdelet

Bocquet²,

Bulet

2020

Rapid Comm Mass Spectrometry

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…Employing MALDI MS for identification takes only a few hours—a reduction in time that can profoundly and positively impact patient outcomes . Several comprehensive reviews of applications of MS in the clinical microbiology lab have been provided . As detailed subsequently in this review, there is keen interest in the use of rapid MS‐based approaches to identify life‐threatening bloodstream infections (eg,).…”

Section: Introductionmentioning

confidence: 99%

“…Combining advances in ionization methods, inexpensive compact mass analyzers and bioinformatics, matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (TOF) MS has emerged over the past two decades as the main tool for rapid and highly effective characterization of microbes . The recent literature contains numerous successful applications of mass spectrometry to the characterization of microbes from all three domains of life and in clinical, environmental, food microbiology, and counter‐bioterrorism settings. In addition to speed, MS affords greater taxonomic resolution—occasionally to the subspecies and even strain levels—than conventional, more time‐consuming approaches .…”

Section: Introductionmentioning

confidence: 99%

Characterization of microbial mixtures by mass spectrometry

Sandrin

Demirev

2017

Mass Spectrometry Reviews

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MS applications in microbiology have increased significantly in the past 10 years, due in part to the proliferation of regulator-approved commercial MALDI MS platforms for rapid identification of clinical infections. In parallel, with the expansion of MS technologies in the "omics" fields, novel MS-based research efforts to characterize organismal as well as environmental microbiomes have emerged. Successful characterization of microorganisms found in complex mixtures of other organisms remains a major challenge for researchers and clinicians alike. Here, we review recent MS advances toward addressing that challenge. These include sample preparation methods and protocols, and established, for example, MALDI, as well as newer, for example, atmospheric pressure ionization (API) techniques. MALDI mass spectra of intact cells contain predominantly information on the highly expressed house-keeping proteins used as biomarkers. The API methods are applicable for small biomolecule analysis, for example, phospholipids and lipopeptides, and facilitate species differentiation. MS hardware and techniques, for example, tandem MS, including diverse ion source/mass analyzer combinations are discussed. Relevant examples for microbial mixture characterization utilizing these combinations are provided. Chemometrics and bioinformatics methods and algorithms, including those applied to large scale MS data acquisition in microbial metaproteomics and MS imaging of biofilms, are highlighted. Select MS applications for polymicrobial culture analysis in environmental and clinical microbiology are reviewed as well.

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MALDI-TOF Mass Spectrometry for Microorganism Identification

Cited by 17 publications

References 222 publications

Physiology of Acetic Acid Bacteria and Their Role in Vinegar and Fermented Beverages

Physiology of Acetic Acid Bacteria and Their Role in Vinegar and Fermented Beverages

Matrix‐assisted laser desorption/ionization mass spectrometry biotyping, an approach for deciphering and assessing the identity of the honeybee pathogen Nosema

Characterization of microbial mixtures by mass spectrometry

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