Magnetic skyrmions are promising candidates as information carriers in logic or storage devices thanks to their robustness, guaranteed by the topological protection, and their nanometric size. Currently, little is known about the influence of parameters such as disorder, defects, or external stimuli on the long-range spatial distribution and temporal evolution of the skyrmion lattice. Here, using a large (7.3 × 7.3 μm 2 ) single-crystal nanoslice (150 nm thick) of Cu 2 OSeO 3 , we image up to 70,000 skyrmions by means of cryo-Lorentz transmission electron microscopy as a function of the applied magnetic field. The emergence of the skyrmion lattice from the helimagnetic phase is monitored, revealing the existence of a glassy skyrmion phase at the phase transition field, where patches of an octagonally distorted skyrmion lattice are also discovered. In the skyrmion phase, dislocations are shown to cause the emergence and switching between domains with different lattice orientations, and the temporal fluctuation of these domains is filmed. These results demonstrate the importance of direct-space and real-time imaging of skyrmion domains for addressing both their long-range topology and stability.skyrmions | Lorentz transmission electron microscopy | skyrmion dynamics | magnetic materials | strongly correlated systems I n a noncentrosymmetric chiral lattice, the competition between the symmetric ferromagnetic exchange, the antisymmetric Dzyaloshinskii-Moriya interaction, and an applied magnetic field can stabilize a highly ordered spin texture, presenting as a hexagonal lattice of spin vortices called skyrmions (1-4).Magnetic skyrmions have been experimentally detected in materials having the B20 crystal structure such as MnSi (5), Fe 1−x Co x Si (6, 7), FeGe (8), and Cu 2 OSeO 3 (9) and, recently, also on systems like GaV 4 S 8 (10) and beta-Mn-type alloys (11). Small-angle neutron scattering studies of bulk solids evidenced the formation of a hexagonal skyrmion lattice confined in a very narrow region of temperature and magnetic field (T-B) in the phase diagram (5, 6). In thin films and thinly cut slices of the same compounds, instead, skyrmions can be stabilized over a wider T-B range as revealed by experiments using cryo-Lorentz transmission electron microscopy (LTEM) (12, 13). Furthermore, it was proposed and recently observed that skyrmions can also exist as isolated objects before the formation of the ordered skyrmion lattice (14, 15). A recent resonant X-ray diffraction experiment also suggested the formation of two skyrmion sublattices giving rise to regular superstructures (16).In a 2D landscape, long-range ordering can be significantly altered by the presence of defects and disorder. Indeed, the competition between order and disorder within the context of lattice formation continues to be an issue of fundamental importance.Condensed matter systems are well known to provide important test beds for exploring theories of structural order in solids and glasses. An archetypal and conceptually relevant example ...