Comparative Wound Healing Activity of two Polyherbal Formulations GC-01 and GC-02 on Experimentally induced Wounds in Rodents

Ashok, Purnima; Gupta, Nishi; Murthy, Niranjana; Shukla, Renuka; Sri, G Rohita

doi:10.5530/ijper.48.2.11

Cited by 3 publications

(5 citation statements)

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“…Simultaneously, the free radicals (lipid peroxidation) were reduced significantly. 51 In the present study on normal wounds, ghee showed increased levels of the antioxidants. The catalase levels in the wound tissue were significantly higher on both days 8 and 16.…”

Section: S367supporting

confidence: 45%

A Biochemical and Molecular Insight to Wound Healing Properties of Traditional Indian Medicines in Normal and Diabetic Rats

Prabhath,

Bhat,

Pai

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Honey, ghee, Glycyrrhiza glabra L. (GG), and Nerium indicum Mill. (NI) have been effectively used in Ayurveda and Indian folk medicine for healing both normal and diabetic wounds. However, the exact biochemical and molecular mechanisms involved are less discussed. The present study explores the wound healing efficacy of these traditional medicines in normal and diabetic rats using biochemical and molecular parameters. Materials and Methods: Normal and Streptozotocin-induced diabetic Wistar rats were used for the study and inflicted with excision wounds. The test materials, i.e., honey, ghee, GG, and NI, were topically applied to the wounds singly and in combinations (H+G: Honey+Ghee and Tot-combination of all test materials). On the 8 th and 16 th days of healing, biochemical and molecular parameters were assessed using the tissues procured from the wound site. We quantified the levels of hydroxyproline and antioxidants (CAT-Catalase, GSH-Glutathione, SOD-Superoxide dismutase, MDA-Malondialdehyde). We also measured the mRNA expression of growth factors, i.e., Transforming Growth Factor-beta (TGFβ), fibroblast growth factor 2 (FGF2), Platelet-Derived Growth Factor (PDGF), and vascular endothelial growth factor (VEGF). Results: Biochemical analysis showed enhanced hydroxyproline levels in both normal and diabetic wounds treated with the test materials. The groups treated with GG in normal and NI and Tot in diabetic wounds showed statistically significant findings (p<0.05). Different study materials showed significant antioxidant properties at different intervals of wound healing. Similarly, different test materials enhanced the expression of different growth factors at the wound site. Conclusion: Result indicates that the wound healing properties of these natural traditional medicines are through their antioxidant and growth factor enhancing capability at the wound site.

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Section: S367supporting

confidence: 45%

A Biochemical and Molecular Insight to Wound Healing Properties of Traditional Indian Medicines in Normal and Diabetic Rats

Prabhath,

Bhat,

Pai

et al. 2024

Ind. J. Pharm. Edu. Res

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“…The results obtained in this study may indicate ischemic damage to vascular endothelial cells caused by the generation of high concentrations of ROS, including iNOS, due to the burn wound, as well as trauma, such as postincision necrectomy (3,8,10). In the state of ischemia and hence an ATP deficit that accompanies a burn, phosphocreatine, which belongs to phosphagens, becomes an energy source for vascular endothelial cells, and thus an increased phosphorylation of creatine by CK occurs (6).…”

Section: Group Of Animalsmentioning

confidence: 74%

“…Assuming that the liver is the main source of AlAT and AspAT, it can be concluded that the reduction of increase in their activity after necrectomy is associated with the reduction of paracrine action of inflammatory mediators formed in the burn wound as a result of its conversion. The activity of these enzymes is influenced mainly by the excessive generation of ROS, including iNOS, whose source is tissue necrosis following the burn (3,8,10). Higher AlAT activity compared to AspAT activity may be due to hepatocyte membrane damage caused by oxidative stress factors.…”

Section: Group Of Animalsmentioning

confidence: 99%

“…This is manifested, among others, by changes in the activity of indicator enzymes and other biochemical blood parameters that determine the state of intracorporeal homeostasis (including those that describe energy, protein, and mineral metabolism, the functional state of the liver and the muscle system). Clinical and experimental studies indicate a significant effect of wound excision/ burn necrosis performed in the hyperacute phase and in the acute phase of burn disease on the maintenance of body homeostasis (3,11). The aim of this study was to evaluate the effect of necrectomy of a burn wound on selected biochemical parameters that describe the state of the organism after the burn and to determine the optimal time for excision of the burn wound.…”

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confidence: 99%

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Effect of early excision of a burn wound on the activity of selected enzymes in pig serum

Aleksiewicz¹,

Lutnicki²,

TUREK³

2022

Medycyna Weterynaryjna

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Thermal stimuli acting on the skin can cause burn wounds. Clinical and experimental studies suggest a beneficial effect of the necrectomy of post burn necrosis in the hyperacute phase and in the acute phase (during the initial period of burn disease). Heat stroke causes hypovolemic shock and influences the energetic status of the liver, which is the main organ responsible for energy production and energy storage. Determination of the activity of indicator enzymes in serum makes it possible to assess the clinical condition of animals. A total of 25 White Large Landrace pigs of both sexes weighing 50 kg (± 2 kg) were used for the study. Burns were made with a software-controlled heating plate on a precisely defined contact surface of 1% of body surface. This resulted in a burn rate of 30% (± 2%) of the body surface with a lesion range between grades III and IIb. Animals from each experimental group were subjected to an infraclavicular necrectomy. Blood was collected and analyzed for AlAT, AspAT, LDH, and CK activities. In all groups, a statistically significant (p <0.05) increase in AlAT, AspAT, LDH, and CK activities was observed already 12 hours after the burn, and there was a very high correlation between the activities of parenchymal enzymes AlAT and AspAT. The study has shown an association between the moment of performing burn wound necrectomy and the activity of AlAT, AspAT, LDH, and CK.

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“… GC01: Butea monosperma Lam ., Symplocos racemosa Roxb ., Mimosa pudica Linn ., Curcuma longa Linn ., Commiphora mukul, Piper longum Linn ., Azadarchta indica Linn ., Pongamia glabra, Cocos nucifera Linn . and Ghrita (Ghee) GC02: Myristica fragrans Houtt., Melia azadarch Linn ., Trichosanthes cucumerina Linn ., Picrorhiza kurroa, Berberis aristata DC ., Curcuma longa Linn ., Hemidesmus indicus R ., Rubia cordifolia Linn ., Terminalia chebula, Vetiveria zizanioides (L.), Madhuca longifolia and Pongamia glabra GC01, GC02 Rats synergistic/potentiative action was observed for individual herb [ 27 ] 22. Butea monosperma, Barleria prionitis, Casuarina equisetifolia, Dalbergia sissoo, and Lagenaria siceraria – Sprague Dawley rats Excision and incision wound model faster wound size reduction and wound closure rates [ 28 ] 23.…”

Section: Resultsmentioning

confidence: 99%

Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives

Dubey¹,

Dixit²

2023

Journal of Ayurveda and Integrative Medicine

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Comparative Wound Healing Activity of two Polyherbal Formulations GC-01 and GC-02 on Experimentally induced Wounds in Rodents

Cited by 3 publications

References 0 publications

A Biochemical and Molecular Insight to Wound Healing Properties of Traditional Indian Medicines in Normal and Diabetic Rats

A Biochemical and Molecular Insight to Wound Healing Properties of Traditional Indian Medicines in Normal and Diabetic Rats

Effect of early excision of a burn wound on the activity of selected enzymes in pig serum

Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives

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