Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives

Dubey, Satish; Dixit, Ashwini Kumar

doi:10.1016/j.jaim.2023.100688

Cited by 13 publications

(8 citation statements)

References 51 publications

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“…The wound healing aspect happens to be the most recognized application of polyherbal formulations amongst its other reported health benefits. 34 Ayurvedic and Indian traditional medicine consider B. monnieri, A. indica and C gigantea as highly regarded medicinal plants owing to their broad-spectrum bioactivities. Existing literature reveals that extracts or active constituents isolated from these plants have wound healing potential.…”

Section: Discussionmentioning

confidence: 99%

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Tanimu,

Zirmire K,

Jamora

et al. 2024

Preprint

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Wound healing is a complex process that requires a well-orchestrated integration of an array of molecular events such as cell migration and proliferation, deposition and remodeling of extracellular matrix components for restoring the structural and functional integrity of the tissue injured. Ayurveda suggests wound healing herbs can achieve enhanced therapeutic effect with reduced toxicity when they are optimally combined in a specific ratio as polyherbal formulation (PHF). The present study was aimed to evaluate the combinatorial wound healing efficacy (invivowound closure and histological changes) of aqueous extracts of three medicinal plants (Bacopa monnieri,Acalypha indicaandCalotropis gigantea). This study also explored how the combination influenced the overall quality of healed wound. Individual wound closure kinetic performance of aqueous plant extracts in C57B/6J mice was assessed using safe concentrations obtained from human adult dermal fibroblast viability assay. The aqueous plant extract combination optimized using response surface methodology was tested forinvivowound closure effectiveness. Quality of healed wound was assessed via Hematoxylin & Eosin and immunohistochemical staining of markers (K1, K5, Loricrin, Ki67, CD31 and collagen1). The combination treatment (B.monnieri-15μg/ml,A.indica-11.59μg/ml,C.gigantea-1μg/ml) contributed to faster wound closure (11 days), improved collagen type I remodeling and angiogenesis, complete re-epithelialization, similar epidermal differentiation pattern as that of individual and control treatments. Ki67 staining revealed no significant increase in cell proliferation in combination compared to individual and control. Findings from the study validates the polyherbal combination′s impressive capability to promote wound healing.

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Section: Discussionmentioning

confidence: 99%

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Tanimu,

Zirmire K,

Jamora

et al. 2024

Preprint

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show abstract

“…Traditionally, polyherbal formulations were employed with the dual purpose of not just restoring a healthy body but also preventing the recurrence of ailments. Another benefit using combination herbs in proportioned rations was to reduce the toxicity associated with individual herbs, consequently enhancing their therapeutic effects [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Acute and Subchronic Toxicological Study of the Cocktail Extract from Curcuma xanthorrhiza Roxb, Phyllanthus niruri L. and Morinda citrifolia L.

Rosidah,

Renggani,

Firdausi

et al. 2024

Journal of Toxicology

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Curcuma xanthorrhiza Roxb, Phyllanthus niruri L., and Morinda citrifolia L. are Indonesian medicinal herbs used empirically as traditional therapeutics for maintaining health. The cocktail extract of these three plants (CECPM) had been developed and demonstrated immunostimulant activity in rats. This study aimed to evaluate the acute and subchronic toxicity of CECPM in vivo. The acute toxicity assay was conducted by orally administering a range dose of CECPM (313, 625, 1250, 2500, or 5000 mg/kg body weight (bw) on female mice once and then evaluating the toxic symptom every day for 14 days later. The chronic toxicity test was carried out by giving various doses of CECPM (600, 800, and 1000 mg/kg·bw) to female and male rats orally continuously for 90 consecutive days. The signs of toxicities were evaluated at the 90- and 28 days postadministration. The acute oral toxicity assays showed that there was no toxic syndrome and mortality found during the period of the experiment. The lethal dose level (LD50) of CECPM was more than 5000 g/kg, which was categorized as practically non-toxic. Meanwhile, in the sub-chronic toxicity study, some parameters tested at 90 days postadministration and after 28 days of withdrawal, such as the body weight, relative organ weight, food intake, hematological and biochemical blood parameters, and also histopathological examination of five primary tissues (heart, liver, kidney, spleen, and lung) revealed no abnormalities. There was no-observed adverse effect level (NOAEL) for the present study of CECPM 1000 mg/kg·bw of the rat. Therefore, it is concluded that the orally administered CECPM was relatively nontoxic during acute and subchronic toxicology studies.

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“…The concept of using a polyherbal formulation involves combining multiple herbs in a specific ratio to create a remedy or treatment that can be employed not only in traditional Thai medicine but also in traditional Chinese, [1][2][3][4] Ayurvedic, 5 Siddha, 6 Unani, 7 and southern African traditional medicinal products. 8 The key concept is that when herbs are used together, they synergistically improve healing properties, leading to a more substantial therapeutic effect than when used individually.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of In Vitro Antioxidant Activities and Toxicity Effects of a Novel Plant-Based Body Lotion from Thai Medicinal Plants

2024

TJNPR

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In Thailand, several polyherbal formulations have been used for treating various diseases, including skin infections. 10 In the present work, a polyherbal formulation for treating skin diseases, known as THR-01, consisting of Rhinacanthus nasutus (Acanthaceae), Quisqualis indica (Combretaceae), Ocimum sanctum (Lamiaceae), Vitex glabrata (Verbenaceae), and Stemona tuberosa (Stemonaceae) was chosen for testing its antioxidant activities and safety. Interestingly, Stemona tuberosa, one of the primary components of this formulation was found to exhibit significant antioxidant properties with low toxicity. Plant roots of the Stemona genus, part of the Stemonaceae family, have historically been a part of traditional Chinese medicine, valued for their capabilities in repelling insects and relieving coughs. The root extracts of these plants can be applied for external use in repelling various insect pests. [11][12][13] Additionally, Stemona root extracts exhibit promising antibacterial properties against some skin infecting pathogens. According to previous research, ethanol extracts of Stemona tuberosa roots have been shown to suppress a broad spectrum of bacteria and fungi. 11 More recent studies have isolated eight new alkaloids from the roots of Stemona tuberosa and one of these compounds showed good anti-inflammatory activity. 14 Therefore, to develop an effective herbal product for external use, especially for the skin, an extract of Stemona tuberosa, the active component of a traditional formula used for treating skin diseases in Southern Thailand, was tested for its antioxidant activity using 1,1diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), and ferric-reducing antioxidant power (FRAP) assays. Its toxicity profile using an in vivo model, Galleria mellonella larvae, was determined, ensuring the safety of the extract for potential cosmetic use, according to the aim of this research. Moreover, consumer acceptance of the resulting body lotion containing various concentrations of Stemona tuberosa extracts was assessed, focusing on sensory attributes such as

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Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives

Cited by 13 publications

References 51 publications

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Acute and Subchronic Toxicological Study of the Cocktail Extract from Curcuma xanthorrhiza Roxb, Phyllanthus niruri L. and Morinda citrifolia L.

Evaluation of In Vitro Antioxidant Activities and Toxicity Effects of a Novel Plant-Based Body Lotion from Thai Medicinal Plants

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