Comparative use of dimethyl sulphoxide (DMSO) in different animal species

Madruga, G.M.; Crivellenti, L.Z.; Borin-Crivellenti, S.; Cintra, C.A.; Gomes, L.G.; Spiller, P.R.

doi:10.17221/176/2015-vetmed

Cited by 11 publications

(6 citation statements)

References 34 publications

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“…The first layer was chosen because it is nontoxic, biocompatible, and able to stabilize fillers to minimize agglomeration [28]. The second surfactant was used to support the first layer and further enhance the performance of Fe 3 O 4 /Ag nanocomposite fillers in biomedical applications [29]. In addition, we developed a simple synthesis method to fabricate Fe 3 O 4 /Ag nanohybrid ferrofluids using an abundant and inexpensive natural resource iron sand as the main precursor.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Fe3O4/Ag nanohybrid ferrofluids and their applications as antimicrobial and antifibrotic agents

Taufiq

Saputro

Susanto

et al. 2020

Heliyon

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To date, the search for creating stable ferrofluids with excellent properties for biomedical application is one of the challenging scientific and practical investigations. In this study, novel Fe 3 O 4 /Ag nanohybrid ferrofluids from iron sand were synthesized using a double-layer method. The Fe 3 O 4 /Ag nanocomposites exhibited stable crystallite sizes of 11.8 12.1 nm and 36.8-37.2 nm for Fe 3 O 4 and Ag, respectively. The lattice parameters of the spinel structure Fe 3 O 4 and face-centered cubic Ag were respectively 8.344 Å and 4.091 Å. With increasing Ag amount, the crystallite phase of Ag in the nanocomposites increased from 40.2% to 77.2%. The XPS results confirmed that Fe 3 O 4 /Ag nanocomposites were successfully prepared, where Fe 3 O 4 mixed well with Ag via strong ionic bonding. The FTIR results confirmed the presence of Fe 3 O 4 /Ag, oleic acid, and dimethyl sulfoxide as the filler, first layer, and second layer, respectively. The as-prepared ferrofluids exhibited superparamagnetic behavior, where the saturation magnetization decreased with increasing Ag content. The Fe 3 O 4 /Ag nanohybrid ferrofluids exhibited excellent antimicrobial performance against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Candida albicans. More importantly, the Fe 3 O 4 /Ag nanohybrid ferrofluids decreased the progression of liver fibrosisrelated inflammation and fibrogenic activity on hepatic stellate cells.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Fe3O4/Ag nanohybrid ferrofluids and their applications as antimicrobial and antifibrotic agents

Taufiq

Saputro

Susanto

et al. 2020

Heliyon

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“…For this purpose, DMSO was used as a vehicle (Soto et al, ) as it is considered a great solvent (Santos, Figueira‐Coelho, Martins‐Silva, & Saldanha, ) has the ability to penetrate biological membranes and cross‐barriers and has anti‐inflammatory and analgesic actions (Jacob & Wood, ; Madruga et al, ). Additionally, DMSO can potentiate the effects of other substances because it facilitates tissue penetration (Paes & Cortês, ).…”

Section: Introductionmentioning

confidence: 99%

“…Necrosis of seminiferous tubules and interstitial cells with fibrous tissue replacement, complete tubular architecture breakdown associated with a leukocyte infiltration, and fibrous and hyaline tissue formation in the histological evaluation were observed, especially at concentrations of 10, 12.5, 15 and 20 mg/kg. For this purpose, DMSO was used as a vehicle (Soto et al, 2009) as it is considered a great solvent (Santos, Figueira-Coelho, Martins-Silva, & Saldanha, 2003) has the ability to penetrate biological membranes and cross-barriers and has anti-inflammatory and analgesic actions (Jacob & Wood, 1967;Madruga et al, 2017). Additionally, DMSO can potentiate the effects of other substances because it facilitates tissue penetration (Paes & Cortês, 2008).…”

Section: Introductionmentioning

confidence: 99%

Calcium chloride combined with dimethyl sulphoxide for the chemical sterilization of dogs

Silva

Paranzini

Franco

et al. 2018

Reprod Domestic Animals

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The aim of this study was to evaluate the effect of intratesticular injection of calcium chloride (CaCl ) combined with dimethyl sulphoxide (DMSO) as a chemical sterilization in dogs. Twelve dogs were divided into two groups: the treated group (n = 6), in which 15 mg/kg of a 7.5% CaCl solution combined with 0.5% DMSO was injected into each testicle (volume range 1.0-4.76 ml); and a control group (n = 6) that received the same volume/kg of 0.9% sodium chloride solution (NaCl). Semen characteristics pre- and post-treatment were evaluated. Serum testosterone concentration was determined before the injection (D-1) and at 15 (D15), 30 (D30) and 60 (D60) days after intratesticular injection. Testicle sizes and local pain were evaluated for 7 consecutive days (D1 to D7) and at D15, D30 and D60 after injection. At D60, testicle histological evaluation was performed after orchiectomy. No pain was observed by testicular palpation, with the exception of one dog in the treated group; this dog then received analgesic therapy. An increase in testicular volume was evident within 24 hr after treatment, followed by gradual reduction for 3 weeks. Five of 6 dogs from the treated group presented azoospermia at D15; the remaining dog presented at D30. There was no significant difference in testosterone concentrations in the treated group during the experimental period. Histological evaluation showed testicular degenerative lesions, especially at the proximal and middle portions. The results indicated that one injection of 7.5% CaCl combined with 0.5% DMSO into each testis is a viable alternative for canine sterilization.

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“…In addition, the author is concerned about the neurotoxicity of DMSO and, consequently, its safety for human use. In their review, Madruga et al (2017) describe some of the adverse effects of DMSO described in the literature, including nausea, vomiting, diarrhea, and severe hemolysis.…”

Section: Discussionmentioning

confidence: 99%

Nanostructured lipid carrier containing hydroxymethylnitrofurazone: preparation, characterization and in vitro and in vivo evaluation of leishmanicidal activity

Souza¹

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A leishmaniose, uma doença tropical negligenciada (DTN), é um conjunto de doenças causadas por protozoários parasitas obrigatórios do gênero Leishmania. E tem como formas principais a leishmaniose cutânea e visceral. O tratamento inclui antimoniais pentavalentes. Esses fármacos apresentam várias desvantagens, como necessidade de administração parenteral, uso de altas dosagens, longa duração do tratamento, toxicidade grave, resistência e eficácia variável. O candidato ao fármaco hidroximetilnitrofural (NFOH), um pró-fármaco derivado do nitrofural, apresentou alta atividade em culturas de células infectadas pelo Trypanosoma cruzi e menor toxicidade quando comparado ao nitrofural. Devido à sua baixa solubilidade em água e biodisponibilidade reduzida, o NFOH falhou nos testes de eficácia in vivo. Os sistemas nanoestruturados de liberação de fármacos têm potencial para superar esses desafios devido às suas vantagens evidentes: maior eficácia terapêutica, menor toxicidade, liberação modificada do fármaco e aumento da absorção gastrointestinal de fármacos com baixa solubilidade em água. O objetivo deste projeto será a preparação e avaliação das características físico-químicas de um carreador lipídico nanoestruturado contendo hidroximetilnitrofural (NLC-NFOH). O NFOH apresentou a maior solubilidade no Miglyol ® 840 entre os lipídios líquidos testados. Para lipídios sólidos, Gelucire ® 50/13 e Precirol ® ATO5 se mostraram mais adequados para a solubilização de NFOH. O NLC-NFOH otimizado consistiu desses três lipídios. Esses lipídios foram selecionados usando Technobis Crystal 16 TM , microscopia e DSC. Diferentes ferramentas de seleção de lipídios forneceram conhecimento científico relevante para o desenvolvimento de NLC. O NLC-NFOH teve z-average de 198,6 ± 5,4 nm, PDI de 0,11 ± 0,01 e potencial zeta de -13,7 ± 0,7 mV. Este estudo permitiu o desenvolvimento por abordagem de Design Space do primeiro NLC-NFOH com potencial para tratar a leishmaniose por via oral. O desenvolvimento de um VIII método bioanalítico sensível utilizando HPLC e a avaliação de algumas figuras analíticas de mérito para a validação permitiram a quantificação de NFOH e NF em soro. O método bioanalítico para análise de NFOH e NF usou coluna de HPLC Zorbax SB-C18, 5 µm, (4,6 x 250 mm). A fase móvel foi constituída por acetonitrila: água (20:80 v / v) com vazão de 1,2 ml / min, com detecção no UV de 370 nm. A linearidade de NFOH e NF foi encontrada na faixa de 0,025-3,0 µg / ml com um coeficiente de correlação de r> 0,98. A precisão foi de 2,44 a 13,77% para NFOH e 2,61 a 18,42%; a precisão foi de 2,66 a 14,28% para NFOH e 2,09 a 19,06% para NF. O método mostrou-se adequado para avaliação efetiva do NFOH no soro. NLC-NFOH (2,8 mg / kg) foi administrado aos animais por gavagem, e o modelo de bloqueio do fluxo de quilomícrons foi realizado com injeção intraperitoneal de cicloheximida. A presença de NFOH no soro foi avaliada com e sem cicloheximida. O ensaio de citotoxicidade de NLC-NFOH e branco-NLC mostrou mais de 90% de células viáveis na concentração m...

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Comparative use of dimethyl sulphoxide (DMSO) in different animal species

Cited by 11 publications

References 34 publications

Synthesis of Fe3O4/Ag nanohybrid ferrofluids and their applications as antimicrobial and antifibrotic agents

Synthesis of Fe3O4/Ag nanohybrid ferrofluids and their applications as antimicrobial and antifibrotic agents

Calcium chloride combined with dimethyl sulphoxide for the chemical sterilization of dogs

Nanostructured lipid carrier containing hydroxymethylnitrofurazone: preparation, characterization and in vitro and in vivo evaluation of leishmanicidal activity

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