Comparative study on the in vitro development of caprine and bovine preantral follicles

Rossetto, R.; Santos, Regiane R.; Silva, G.M.; Duarte, A.B.G.; Silva, C.M.G.; Campello, C.C.; Figueiredo, J.R.

doi:10.1016/j.smallrumres.2013.03.004

Cited by 13 publications

(16 citation statements)

References 14 publications

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“…Although antral cavities are usually established when the follicles reach at least 200 μm in diameter [ 24 , 36 ], as we mentioned previously, large secondary follicles (greater than 190 μm in diameter) have been mechanically isolated from bovine ovaries [ 37 – 40 ], as well as from the ovaries of other species such as goats [ 16 , 41 , 42 ] and sheep [ 14 , 15 ].…”

Section: Reviewmentioning

confidence: 99%

“…Although primordial and primary follicles can easily be isolated from bovine ovaries using mechanical or enzymatic methods, mostly small (diameter ≤150 μm) and large (diameter >150 μm) secondary follicles have commonly been used for this in vitro culture system [ 37 – 40 , 79 ]. In the bovine species, several studies have used in vitro culture of isolated follicles (Table 2 ).…”

Section: Reviewmentioning

confidence: 99%

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In vitro culture of bovine preantral follicles: a review

Araújo

Gastal

Figueiredo

2014

Reprod Biol Endocrinol

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Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral follicles and formation of early antral follicles from large (secondary) preantral follicles after in vitro culture. Therefore, this review will highlight the basic aspects of bovine folliculogenesis by focusing on preantral follicles, the methods of harvesting preantral follicles, the main results from in vitro follicular culture during the last 20 years, and the potential candidate substances (basic supplements, growth factors, and hormones) for improving the efficiency of in vitro follicle growth.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

In vitro culture of bovine preantral follicles: a review

Araújo

Gastal

Figueiredo

2014

Reprod Biol Endocrinol

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“…Interestingly, at D6, JI0.3 showed a higher percentage of antrum formation. This finding is important because the ability to form an antrum is considered as a good marker of follicular functionality [56], as the mechanisms by which small cavities of fluid develop inside the follicle to form the antral cavity are related to the secretion of osmotically active molecules into small spaces between the granulosa cells [57]. Recently, a study using Amburana cearensis showed that its metabolite protocatechuic acid improves the antrum formation after 18 days of ovine preantral follicle culture in vitro [22].…”

Section: Discussionmentioning

confidence: 99%

Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis

et al. 2018

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The present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-MEM+ (Control), α-MEM+ supplemented with 100 ng/mL recombinant bovine follicle stimulating hormone (FSH) or with 0.3, 1.25, or 2.5 mg/mL of J. insularis extract (JI0.3, JI1.25, and JI2.5, respectively). Culture medium collected every 2 days was used to measure ROS levels. At the end of the culture period, cumulus oocytes complex (COCs) were collected and matured in vitro. Follicular walls were used for mRNA quantitation. JI0.3 led to a higher (P < 0.05) percentages of intact follicles than other groups after 18 days of culture. While follicular diameter remained unchanged from Day 6 onwards with JI0.3 and FSH, percentages of antral cavity formation were higher (P < 0.05) with JI0.3 at Day 6 than in all other treatments. No differences were observed between controls and treatment groups regarding ROS levels and mRNA expression of genes. Viability of resulting oocytes was higher (P < 0.05) in JI0.3 compared to FSH. Interestingly, in control experiment, supplementation of maturation medium with JI0.3 led to higher (P < 0.05) percentages of metaphase II compared to controls. Although more validations will be needed, it seems that this natural extract could be used as a cheap and easily available alternative to commercial FSH.

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“…Several substrates are used for the supplementation of culture medium, such as pyruvate, hypoxanthine, glutamine, ITS, and BSA. The medium used for the in vitro culture are rich in oxygen, nutrients and other elements present in the ovarian cortex (Rossetto et al , 2013), and all of these components need to be considered for a correct understanding of follicular physiology.…”

Section: Discussionmentioning

confidence: 99%

Influence of follicle-stimulating hormone concentrations on the integrity and development of bovine follicles cultured in vitro

Bizarro-Silva

Santos

Gerez

et al. 2018

Zygote

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SummaryThis study investigated the in vitro culture of bovine follicles included in ovarian tissue for 2 or 6 days (D2 or D6), with the addition of different concentrations of follicle-stimulating hormone (FSH) (0, 10, 50, 100 or 200 ng/ml). Data were compared for follicular development, morphological integrity and diameter of follicles and oocytes. Ovaries (n = 10) from Nelore cows (n = 5) were divided into fragments (n = 11 per ovary) and were immediately fixed in Bouin’s solution (D0) or were individually cultured for 2 or 6 days in one of the described concentrations of FSH and then processed for histology. Compared with the rates of follicular development at D2 for minimal essential medium (MEM) (75.0%) and 50 ng/ml of FSH (71.1%), the best rates of follicular development at D2 were obtained with 10 (84.7%), 100 (87.5%) and 200 ng/ml of FSH (85.0%; P<0.05). After 6 days of cultivation, there were no differences among treatments regarding follicular growth. The morphological integrity of preantral follicles was better maintained by 100 ng/ml FSH for 2 and 6 days of cultivation (51.2 and 40.4%, respectively; P<0.05) than that for MEM (D2: 30.9%, D6: 20.8%), 10 (D2: 39.2%, D6: 22.8%), 50 (D2: 30.4%, D6: 28.8%) and 200 ng/ml FSH (D2: 45.2%, D6: 36.8%). FSH at 100 ng/ml provided the highest mean diameter averages: 34.5±10.8 µm at D2 and 33.2±12.5 µm at D6 (P<0.05). We concluded that the medium supplemented with 100 ng/ml FSH during in vitro culture provided appropriate conditions for the development and morphological integrity of preantral follicles in cattle.

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Comparative study on the in vitro development of caprine and bovine preantral follicles

Cited by 13 publications

References 14 publications

In vitro culture of bovine preantral follicles: a review

In vitro culture of bovine preantral follicles: a review

Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis

Influence of follicle-stimulating hormone concentrations on the integrity and development of bovine follicles cultured in vitro

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