Comparative study on some amylases in mammalian blood serum

A, Vacíková

doi:10.1016/0300-9629(71)90286-6

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Total RNA (5-20 ,tg) was reverse-transcribed with M-MLV reverse transcriptase (BRL) and P2 or P3 primers according to the procedure described in the cDNA synthesis manual provided by BRL. Reverse transcribed RNA (cDNA) was amplified (26), with Taq DNA polymerase (Cetus), gene-specific primers (64 pM of each P1 and P2) and dNTPs (800 ,uM each) during [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] rounds on a Perkin-Elmer Cetus PCR DNA Thermocycler. Each cycle consisted of denaturation at 94°C for 1 min, annealing at 50°C for 1 min and extension at 630 for 3 min.…”

Section: Rt-pcrmentioning

confidence: 99%

α-Amylase gene transcription in tissues of normal dog

Mocharla

Hodes

1990

Nucl Acids Res

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We studied the distribution of alpha-amylase mRNA in normal dog tissues by northern blotting (NB) and reverse transcription-polymerase chain reaction (RT-PCR) with human pancreatic (AMY2) and salivary (AMY1) alpha-amylase cDNA-specific primers. Analysis of poly(A+) RNA from various normal tissues by NB indicated the presence of detectable levels of alpha-amylase mRNA transcripts only in pancreas. Dot-blot analysis of DNA amplified with primers common to both (human) isoamylase mRNAs showed presence of alpha-amylase gene transcripts not only in pancreas but also in liver, small intestine, large intestine and fallopian tube. Traces of amylase gene transcripts were also observed in ovary, uterus and lung. Interestingly, amylase transcripts were not detectable in the parotid gland by NB or RT-PCR. We have also localized alpha-amylase mRNA transcripts to dog pancreas by in situ transcription and in situ hybridization. Our results suggest that there is high degree of homology between the alpha-amylase mRNA sequences in dog and human at least in the exon 3-4 regions of the human gene.

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