α-Amylase gene transcription in tissues of normal dog

Mocharla, Hanna; Mocharla, Raman; Hodes, M. E.

doi:10.1093/nar/18.4.1031

Cited by 21 publications

(10 citation statements)

References 42 publications

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“…Among the test animal salivas, dog and cat salivas are reported to have no salivary amylases activity (14). Amylase transcripts were not detectable in the dog parotid gland (18). …”

Section: Resultsmentioning

confidence: 99%

Applicability of Two Commercially Available Kits for Forensic Identification of Saliva Stains

Pang¹,

Cheung²

2008

Journal of Forensic Sciences

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The RSID-saliva test and the SALIgAE-saliva test are two recently developed forensic saliva detection kits. In this study, we compared the sensitivity and the specificity of the two test kits with the Phadebas amylase test by analyzing amylases from various sources including human, animals, plants, and micro-organism. The data demonstrate that the RSID-saliva test and the SALIgAE-saliva test offer higher sensitivity and specificity for the detection of saliva than the Phadebas amylase test. The detection limits of the RSID-saliva test, the SALIgAE-saliva test, and the Phadebas amylase test equate to 10, 4, and 1000 nL, respectively for human saliva. The RSID-saliva test and the SALIgAE-saliva test were further evaluated by analyzing semen, vaginal secretion, breast milk, blood, urine, sweat, and feces. The results of the two tests are in good agreement. The two tests reacted with urine, breast milk, and feces, but not with semen, vaginal secretion, blood, and sweat.

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“…Among the test animal salivas, dog and cat salivas are reported to have no salivary amylases activity (14). Amylase transcripts were not detectable in the dog parotid gland (18). …”

Section: Resultsmentioning

confidence: 99%

Applicability of Two Commercially Available Kits for Forensic Identification of Saliva Stains

Pang¹,

Cheung²

2008

Journal of Forensic Sciences

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“…Whether this is due to important differences in the populations of patients in previous human studies and dogs included in the present study or to a true diminished risk of HES-associated AKI in dogs compared to people is unclear. Explanations for possible interspecies differences may be a greater capacity to degrade HES in dogs due to greater ␣-amylase activity (the enzyme responsible for HES breakdown) 49 or diminished accumulation due to a higher rate of elimination compared to people, proven with high molecular weight HES (8 d vs 13 d, respectively). 45 In our study population, the median daily dose of HES administered was significantly lower than the maximal daily dose for people recommended by the manufacturers at the time of administration of the product (50 mL/kg/d).…”

Section: Discussionmentioning

confidence: 99%

Retrospective evaluation of the effects of administration of tetrastarch (hydroxyethyl starch 130/0.4) on plasma creatinine concentration in dogs (2010–2013): 201 dogs

Yozova

Howard

Adamik

2016

J Vet Emergen Crit Care

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“…Although the enzyme activity was detected in the normal renal tissue, the AMY1A or AMY2 gene expression was undetected by reverse transcription polymerase chain reaction. 28 …”

Section: Discussionmentioning

confidence: 99%

Amylase α-1A (AMY1A)

Jain

Amin

Acquafondata

et al. 2013

American Journal of Surgical Pathology

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Chromophobe renal cell carcinoma (ChRCC) and oncocytoma present with a perplexing overlap of morphologic and immunohistochemical features. ChRCC have deletions in the 1p21.1 region including the amylase α-1A gene (AMY1A). No such deletions are found in oncocytoma. Instead, oncocytomas shared other deletions on chromosome 1: 1p31.3, 1q25.2, and 1q44. We performed AMY1A immunostaining on 75 oncocytomas (57 tissue microarray [TMA] cores, 18 whole slides) and 54 ChRCCs (20 TMA cores, 34 whole slides). Staining was assessed using the H-score method. The intensity was graded as follows: no staining=0, weak=1, moderate=2, and strong=3. The AMY1A immunostain preferentially stained the distal tubules and collecting ducts of normal kidney. All oncocytomas (100%) expressed AMY1A with an H-score that varied from 100 to 300 (mean 205). Mild to moderate heterogeneity in staining intensity was noted within a given oncocytoma. For oncocytomas, 87% (65/75) cases had H-scores of at least 120 with a mean score of 221. Notably, the 13% (10/75) of oncocytoma cases that had an H-score of 100 were derived from the TMA. A total of 87% (47/54) of the ChRCC cases were negative for the AMY1A immunostain. Of the ChRCC cases, 4% (2/54) showed very weak cytoplasmic staining (H-score of 70 each), which was less than the lowest H-score of oncocytoma cases. All 5 cases of ChRCC, which showed an H-score of 100 or more, were referred to as eosinophilic variants of ChRCC. Three of these 5 cases showed a very nondescript, diffuse staining of the cytoplasm. Two of these 5 cases showed an H-score of 130. We think that as the staining pattern of these 2 cases is similar to that of oncocytoma, they should be put in a category of renal oncocytic neoplasms favoring oncocytoma. This result shows that AMY1A staining could be very helpful in further classifying even a subset of the eosinophilic variants of ChRCC. The difference between ChRCC and oncocytoma was statistically significant (χ2 test, P<0.0001). All cases of clear cell RCC and papillary RCC were negative for AMY1A expression. Overall, sensitivity and specificity of AMY1A staining for oncocytoma was 100% (95% confidence interval, 0.95–1.00) and 96.75% (95% confidence interval, 0.93–0.99), respectively. Similarly, the sensitivity and specificity for distinguishing oncocytoma from ChRCC was 100% (95% confidence interval, 0.95–1.00) and 90.74% (95% confidence interval, 0.80–0.97), respectively. These data show that the novel marker AMY1A can be of great diagnostic utility when trying to differentiate ChRCC (classic and eosinophilic variant) and oncocytoma.

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α-Amylase gene transcription in tissues of normal dog

Cited by 21 publications

References 42 publications

Applicability of Two Commercially Available Kits for Forensic Identification of Saliva Stains

Applicability of Two Commercially Available Kits for Forensic Identification of Saliva Stains

Retrospective evaluation of the effects of administration of tetrastarch (hydroxyethyl starch 130/0.4) on plasma creatinine concentration in dogs (2010–2013): 201 dogs

Amylase α-1A (AMY1A)

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