Comparative Study of Survival of Autologous Adipose Tissue Taken and Transplanted by Different Techniques

Nguyen, Abram; Pasyk, Krystyna A.; Bouvier, Theresa N.; Hassett, Cheryl A.; Argenta, Louis C.

doi:10.1097/00006534-199003000-00008

Cited by 49 publications

(46 citation statements)

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“…a At 9 weeks postoperatively, the grafted fat specimen is removed. b The specimen from the right side is grossly larger than that from the left side absorption, overcorrection or retouch grafting is commonly performed to resolve the aforementioned disadvantages [5,6]. This also is accompanied by the troublesome process collecting adipose cells for retouch grafting.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Botulinum Toxin A on Fat Graft Survival

et al. 2012

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Section: Discussionmentioning

confidence: 99%

“…However, it has the disadvantage that its effects cannot be accurately predicted due to a higher difference in absorption rates. Accordingly, in a clinical setting, overcorrections or retouch grafting are commonly performed to resolve this disadvantage [5,6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Botulinum Toxin A on Fat Graft Survival

et al. 2012

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“…Autologous flaps are based on skin, fat and muscle (Cordeiro 2008;Kanchwala et al 2009). The use of free non-vascularized fat grafts is limited by shrinkage, fibrosis and oil cyst formation (Fagrell et al 1996;Kononas et al 1993;Nguyen et al 1990;Smahel 1989). Vascularized fat grafts on the contrary show good results, such as the deep inferior epigastric perforator flap, which is a skin and fat graft that is removed from the lower abdomen and transferred to the chest to reconstruct a breast after mastectomy without the sacrifice of any of the abdominal muscles.…”

Section: Introductionmentioning

confidence: 99%

“…Mature fat cells have inferior viability, low expandability and inferior volume stability (Tanzi and Fare 2009). Experimentally, hAPCs are more resistant to stress and hypoxia (Nguyen et al 1990). The easy harvesting via liposuctions and the ability of proliferation and adipogenic differentiation predestines hAPCs as the ideal cell source of adipose tissue engineering (Schreml et al 2009; Roncari 1977, 1982).…”

Section: Introductionmentioning

confidence: 99%

Engineering of vascularized adipose constructs

et al. 2011

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Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration. Sponge-like polyurethane scaffolds were prepared by mold casting with methylal as foaming agent, whereas polycaprolactone scaffolds with highly regular stacked-fiber architecture were fabricated with fused deposition modeling. Both scaffold types were seeded with human adipose tissue-derived precursor cells, cultured and implanted in nude mice using a femoral arteriovenous flow-through vessel loop for angiogenesis. In vitro, cells attached to both scaffolds and differentiated into adipocytes. In vivo, angiogenesis and adipose tissue formation were observed throughout both constructs after 2 and 4 weeks, with angiogenesis being comparable in seeded and unseeded constructs. Fibrous tissue formation and adipogenesis were more pronounced on polyurethane foam scaffolds than on polycaprolactone prototyped scaffolds. In conclusion, both scaffold designs can be effectively used for adipose tissue engineering.

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“…While fat tissue has many characteristics, making it a unique source of cells and tissue for the reconstruction of soft tissue defects, its limited tolerance to ischemia still causes problems [18]. After resorption rates of 20-80% have been described in earlier publications [19][20][21], just recently the first objective studies based on MRI volumetry have shown a volume survival rate from 64 to 76% [1,2,22]. Autologous fat transplantation results in ischemia and hypoxia of adipose tissues [23].…”

Section: Discussionmentioning

confidence: 99%

Apoptosis in extracorporeal preserved inguinal fat flaps of the rat

et al. 2012

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Fat cells are fragile cells with a short life span outside the body. Ways to reduce cell death in a biochemical way are almost unknown due to scarce information on the type of cellular death that is induced in fat tissue. This study was designed to investigate the apoptotic pathways of fat tissue in a permanent perfusion bioreactor system with the Hannover preservation solution and the Eurocollins solution in fat flaps of rats. In Lewis rats, the inguinal adipofascial flaps were elevated bilaterally and placed in a bioreactor at 37°C. To detect caspases 3, 8, 9 and 12, immunofluorescence stains of fat tissue specimen were analysed at several time points after preservation of flaps were placed in Hannover solution and Eurocollins solution for 10 days. An additional visual assessment of viability by a calcein based life/dead test was performed. It revealed a superior viability of the adipose tissue preserved in Hannover solution. Immunofluorescence staining demonstrated that apoptotic pathways via mitochondria, endoplasmatic reticulum and death receptors were activated, as Caspases 8, 9 and 12 were detected. Caspase 3 as an effector in the common apoptotic pathway was detected as well. Adipose tissue preserved at 37°C ex vivo in a bioreactor system undergoes apoptosis. Immunofluorescence examination of the fat tissue preserved ex vivo revealed that apoptotic pathways via mitochondria, endoplasmatic reticulum and death receptors are being activated. Significantly less activation of Caspase 3, 8, 9 and 12 in flaps preserved in Hannover solution in comparison to Eurocollins was found, supporting the anti apoptotic characteristics of Hannover solution. Based on these findings, further research to modify the apoptotic pathways to ameliorate viability of fat tissue can be performed.

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Comparative Study of Survival of Autologous Adipose Tissue Taken and Transplanted by Different Techniques

Cited by 49 publications

References 0 publications

The Effect of Botulinum Toxin A on Fat Graft Survival

The Effect of Botulinum Toxin A on Fat Graft Survival

Engineering of vascularized adipose constructs

Apoptosis in extracorporeal preserved inguinal fat flaps of the rat

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