Comparative study of poly(vinyl alcohol)‐based support materials for the immobilization of glucose oxidase

Abstract

BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chemically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-PVA hybrid material.

Graphene modified electrospun poly(vinyl alcohol) nanofibrous membranes for glucose oxidase immobilization

“…In this study, the glucose biosensor's electrochemical measurements were made using a three-electrode system with a potentiostat (CH Instruments Electrochemical Analyzer) [3]. The auxiliary electrode was a Pt wire, and an Ag/AgCl electrode was used as a reference.…”

“…These results reveal a MichaelisMenten dynamic characteristic. The apparent Michaelis-Menten constant (K m app ) can be calculated from the Hanes-Woolf regression [3] as shown by Equations (5)- (7):…”

“…In attempts to retain enzymatic activity and stability various molecules have been used to immobilize enzymes on different substrates, e.g. poly(vinyl alcohol) (PVA) [3], poly(ethylene oxide) (PEO) [4], chitosan [5], polymethylmethacrylate (PMMA) [6], poly(vinyl pyrrolidone) (PVP) [7] and polyurethane (PU) [8]. PVA is often used as an immobilization matrix because of its inherent nontoxicity, high thermal stability, good biocompatibility and its desirable physical properties such as its elastic nature, good film forming properties, and its high degree of swelling in aqueous solutions -all of which contribute to making it a good matrix for enzyme immobilization [9].…”

“…PVA is often used as an immobilization matrix because of its inherent nontoxicity, high thermal stability, good biocompatibility and its desirable physical properties such as its elastic nature, good film forming properties, and its high degree of swelling in aqueous solutions -all of which contribute to making it a good matrix for enzyme immobilization [9]. Different methods, such as the cross-linking of PVA [10], freeze-thawed PVA [11], enzyme encapsulation in PVA/silicate hybrid materials [3], have been successfully employed to immobilize the enzyme molecules in various membranes. However, because of the compaction and low-conductivity of the PVA membrane, substrate infiltration and electron transfer between the enzyme membrane and the electrode remains problematic [12].…”

Enhanced Glucose Sensor Linearity Using Poly(Vinyl Alcohol) Hydrogels

“…Apart from this drug-releasing ability, PVA hydrogels afford versatile cross-linking via freeze-thaw cycling that alleviates the use of reactive cross-linking agents (which can lead to a number of unwanted side reactions) 30,36,37 while maintaining a modulus similar to that of human tissue. Moreover, the well-known permeability of three to five FT-cycled PVA hydrogels for glucose and proteins 38 renders any PVA-induced sensitivity loss minimal.…”