Development and characterization of a novel bioactive polymer with antibacterial and lysozyme‐like activity

Guascito, Maria Rachele; Chirizzi, Daniela; Malitesta, Cosimino; Giotta, Livia; Mastrogiacomo, Disma; Valli, Ludovico; Stabili, Loredana

doi:10.1002/bip.22404

Cited by 17 publications

(11 citation statements)

References 51 publications

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“…Poly(ε-caprolactone) (PCL) is a commonly used biocompatible and biodegradable polymer, having extensive applications in medical fields [1][2][3][4]. PCL is semi-crystalline, brittle, and hydrophobic.…”

Section: Introductionmentioning

confidence: 99%

Properties of Electrospun Nanofibers of Multi-Block Copolymers of [Poly-ε-caprolactone-b-poly(tetrahydrofuran-co-ε-caprolactone)]m Synthesized by Janus Polymerization

Shah

Yang

et al. 2017

Polymers

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Abstract:Novel biodegradable multiblock copolymers of [PCL-b-P(THF-co-CL)] m with PCL fractions of 53.3 and 88.4 wt % were prepared by Janus polymerization of ε-caprolactone (CL) and tetrahydrofuran (THF). Their electrospun mats were obtained with optimized parameters containing bead-free nanofibers whose diameters were between 290 and 520 nm. The mechanical properties of the nanofiber scaffolds were measured showing the tensile strength and strain at break of 8-10 MPa and 123-161%, respectively. Annealing improved their mechanical properties and their tensile strength and strain at break of the samples increased to 10-13 MPa and 267-338%, respectively. Due to the porous structure and crystallization in nanoscale confinement, the mechanical properties of the nanofiber scaffolds appeared as plastics, rather than as the elastomers observed in bulk thermal-molded film.

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Section: Introductionmentioning

confidence: 99%

Properties of Electrospun Nanofibers of Multi-Block Copolymers of [Poly-ε-caprolactone-b-poly(tetrahydrofuran-co-ε-caprolactone)]m Synthesized by Janus Polymerization

Shah

Yang

et al. 2017

Polymers

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“…In combination with PEDOT:PSS, this polymer enhances the mechanical properties as well as durability and flexibility of PEDOT:PSS composite films [21,22]. Additionally, PVA has proven its compatibility as an immobilization matrix for enzymes [23][24][25] making PVA an attractive material for applications in the biomedical field [26][27][28][29].…”

mentioning

confidence: 99%

Activity determination of FAD‐dependent glucose dehydrogenase immobilized in PEDOT: PSS‐PVA composite films for biosensor applications

Riegel

Borzenkova

Haas

et al. 2016

Engineering in Life Sciences

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In this study, we propose an entirely new combination of materials as a novel formulation devoted for future generation of glucose biosensor. We combined poly(3,4‐ethylenedioxythiophene:polystyrene sulfonate) and poly(vinyl alcohol) as conducting matrix and immobilized the highly active and pioneering enzyme flavin adenine dinucleotide‐dependent glucose dehydrogenase (FAD‐GDH) into it. In this context, a good processability of the materials and the preservation of the enzymatic activity are critical steps to provide full device function later on. Thus, we thoroughly characterized the proposed pairing of materials: the influence of the composition on the film‐forming properties was investigated by scanning electron microscopy, optical microscopy, and photography; for the assessment of the enzymatic activity, we developed a spectrophotometric method. A great dependence is observed between activity and composition, ranging from inactive (1.6% retained activity) to highly active (91.5%). A high share of enzyme led to a higher enzymatic activity as long as the necessary condition of a pH higher than 6 was met. These first and fundamental findings can now be used as guidelines to tailor mediator‐free biosensors with enhanced selectivity and to enable the design of devices with improved performance.

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“…The discussed tendency of PVA not only being a good film former but also promoting the stabilization of the enzyme within the composite films is continued when looking at TS1 (diamond half up). This system consists of the highest amount of PVA with 49 w%, which means that good and homogeneous films could be processed (cp.…”

Section: Parameters Of Adjusted Correlation Used To Describe the Risementioning

confidence: 99%

Commentary regarding: “Activity determination of FAD‐dependent glucose dehydrogenase immobilized in PEDOT: PSS‐PVA composite films for biosensor applications”

Walz

Liebl

Schmitt

et al. 2019

Engineering in Life Sciences

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In the light of continuous improvement and optimization, recent experiments that the authors conducted give new insights into the applied evaluation method of Riegel et al. [1]: Thorough investigations of the previous results regarding the usage of the Lowry Assay showed discrepancies in the determination of the released amount of protein in the analysis solution. The accurate quantification of this parameter is crucial as it directly influences the calculation of the residual enzymatic activity. In concrete terms, this finding has a major impact on the presented and discussed results in the article “Activity determination of FAD‐dependent glucose dehydrogenase immobilized in PEDOT: PSS‐PVA composite films for biosensor applications” [1]. Thus, this commentary addresses the new insights concerning the applied evaluation method, explains necessary revisions and discusses new conclusions derived from the adjusted evaluation method.

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Development and characterization of a novel bioactive polymer with antibacterial and lysozyme‐like activity

Cited by 17 publications

References 51 publications

Properties of Electrospun Nanofibers of Multi-Block Copolymers of [Poly-ε-caprolactone-b-poly(tetrahydrofuran-co-ε-caprolactone)]m Synthesized by Janus Polymerization

Properties of Electrospun Nanofibers of Multi-Block Copolymers of [Poly-ε-caprolactone-b-poly(tetrahydrofuran-co-ε-caprolactone)]m Synthesized by Janus Polymerization

Activity determination of FAD‐dependent glucose dehydrogenase immobilized in PEDOT: PSS‐PVA composite films for biosensor applications

Commentary regarding: “Activity determination of FAD‐dependent glucose dehydrogenase immobilized in PEDOT: PSS‐PVA composite films for biosensor applications”

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