Comparative study of methods for quantitation of lactate dehydrogenase isozymes

Pj, Fritz; Wj, Morrison; El, White; Es, Vesell

doi:10.1016/0003-2697(70)90381-7

Cited by 81 publications

(17 citation statements)

References 14 publications

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“…Five distinct isozyme bands were produced and the gels were analyzed quantitatively with a Gilford gel scanner at an absorbance of 530 nm. Fritz et al (24), Dietz and Lubrano (22) and Dietz et al (23) have shown that the reduction of the colorless tétrazolium to the purple formazan is proportional to the amount of LDH activity in each band when the activity of the sample is limited to between 0.001 and 0.002 IU per gel. Tracings that resulted from the scan were there fore quantitatively related to the amount of LDH activity m each band.…”

Section: Resultsmentioning

confidence: 99%

Origin of Lactate Dehydrogenase in Amniotic Fluid

Garver¹,

Marchese²,

Wineman³

et al. 1980

Enzyme

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The origin of protein including enzymes in amniotic fluid is of clinical and research interest. 8 patients were evaluated, with their informed consent, prior to therapeutic abortion. Amniotic fluid, placentas and blood were obtained and lactate dehydrogenase (LDH) isozyme patterns of cell-free amniotic fluid was compared to the LDH patterns of uncultured amniotic fluid cells, maternal serum and RBC, placentas and placental membranes. The LDH isozyme pattern of amnion closely corresponded to that of cell-free amniotic fluid and represents the major source of this enzyme in amniotic fluid.

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Section: Resultsmentioning

confidence: 99%

Origin of Lactate Dehydrogenase in Amniotic Fluid

Garver¹,

Marchese²,

Wineman³

et al. 1980

Enzyme

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“…The enzymes were all supplied by Sigma Chemical Co., London. Lactate dehydrogenase was assayed following the method of Fritz et al [17] but using 0.02 M phosphate buffer, pH 7.4, instead of Tris. Adenosine deaminase was assayed by the method of Kalckar [18].…”

Section: Methodsmentioning

confidence: 99%

A simple general method for the preparation of ‘6‐immobilized’ analogues of AMP, ATP, NAD and of other adenine‐containing compounds for affinity chromatography

Barry

O'Carra

1973

FEBS Letters

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“…Polyacrylamide gel electrophoresis of lactate dehydrogenase isoenzymes was carried out as described by Fritz et al [21].…”

Section: Electrophoresismentioning

confidence: 99%

Presence of lactate dehydrogenase-containing vesicles in an intramembranous ossifying tissue: New-born mouse calvaria

1993

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Lactate dehydrogenase-containing vesicles have been isolated from the extracellular matrix of the calvaria of new-born mice. The calvariae, intramembranous ossification tissue, were removed from 2-day-old mice, followed by the separation of the extracellular matrix vesicle fraction after collagenase digestion. Lactate dehydrogenase-containing vesicles with a density different from that of matrix vesicles were detected in the matrix vesicle fraction. Lactate dehydrogenase in these vesicles did not result from cell lysis and vesicle capture during the preparation of the matrix vesicle fraction. The isoenzyme pattern of lactate dehydrogenase in lactate dehydrogenase-containing vesicles was nearly identical to that of cytosolic lactate dehydrogenase of the cell fraction. Other cytosolic enzymes were not detected in lactate dehydrogenase-containing vesicles, suggesting the presence of a mechanism for specific uptake of cytosolic lactate dehydrogenase during the in vivo formation of the vesicles. This is the first report on the presence of lactate dehydrogenase-containing vesicles in the intramembranous ossification site.

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Comparative study of methods for quantitation of lactate dehydrogenase isozymes

Cited by 81 publications

References 14 publications

Origin of Lactate Dehydrogenase in Amniotic Fluid

Origin of Lactate Dehydrogenase in Amniotic Fluid

A simple general method for the preparation of ‘6‐immobilized’ analogues of AMP, ATP, NAD and of other adenine‐containing compounds for affinity chromatography

Presence of lactate dehydrogenase-containing vesicles in an intramembranous ossifying tissue: New-born mouse calvaria

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