Presence of lactate dehydrogenase-containing vesicles in an intramembranous ossifying tissue: New-born mouse calvaria

Naito, Torn; Yokota, Makoto; Noguchi, T.

doi:10.1007/bf01352017

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…This hypothesis can be supported by literature that reports a potential bone inductive capacity of porous ceramics and extracellular matrix. [25][26][27][28] However, the scaffolds without cells failed to show bone induction, and a previous study showed that identically cultured TE constructs that contained extracellular matrix and were devitalized before implantation also failed to show any bone. 5,6 Therefore, the hypothesis of an osteoinductive mechanism without a contribution of the cells is not plausible.…”

Section: Discussionmentioning

confidence: 92%

Optimization of bone‐tissue engineering in goats

et al. 2004

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Successful bone-tissue engineering (TE) has been reported for various strategies to combine cells with a porous scaffold. In particular, the period after seeding until implantation of the constructs may vary between hours and several weeks. Differences between these strategies can be reduced to (a) the presence of extracellular matrix, (b) the differentiation status of the cells, and (c) the presence of residual potentially immunogenic serum proteins. These parameters are investigated in two types of calcium phosphate scaffolds in a goat model of ectopic bone formation. Culture-expanded bone-marrow stromal cells from eight goats were seeded onto two types of hydroxyapatite granules: HA60/400 (60% porosity, 400-microm average pore size) and HA70/800. Scaffolds seeded with cells and control scaffolds were cultured for 6 days in medium containing autologous or semisynthetic serum, in the presence or absence of dexamethasone. Other scaffolds were seeded with cells just before implantation in medium with or without serum. All conditions were implanted autologously in the paraspinal muscles. After 12 weeks, bone had formed in 87% of all TE constructs, as demonstrated by histology. Histomorphometry indicated significantly more bone in the HA70/800 scaffolds. Furthermore, a significant advantage in bone formation was found when the constructs had been cultured for 6 days. In conclusion, both scaffold characteristics (porosity) and TE strategy (culturing of the constructs) were demonstrated to be important for bone TE.

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Section: Discussionmentioning

confidence: 92%

Optimization of bone‐tissue engineering in goats

et al. 2004

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A New Type of Matrix Vesicles is Found in Fetal Bovine Tracheal Cartilage

Maki¹,

Hayashi²,

Nishioka³

et al. 2000

Connective Tissue Research

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Using 4-month-old fetal bovine tissue, the properties of the tibia epiphyseal cartilage matrix vesicles, a type of endochondral ossification tissue, were compared with those from tracheal cartilage. The matrix vesicle fractions, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation. Alkaline phosphatase activity, protease activity, and lacatate dehydrogenase activity were assayed for the marker enzyme of the matrix vesicles. Matrix vesicles containing alkaline phosphatase, metalloprotease, and lacatate dehydrogenase were found in the tibia epiphyseal cartilage at a density of 1.11 g/ml. In surprising contrast, we also found matrix vesicle-like vesicles with a high density of 1.24 g/ml in the tracheal cartilage. These also contained alkaline phosphatase and lactate dehydrogenase, but not metalloprotease. The electrophoretic profiles of the lactate dehydrogenase isoenzymes from the matrix vesicle and matrix vesicle-like vesicles were identical with those of chondrocyte cytosolic lactate dehydrogenase. Aldolase, aspartate: 2-oxoglutarate aminotransferase, alanine: 2-oxoglutarate aminotransferase, glucose-6-phosphatase, glutamate dehydrogenase, catalase, and cytosolic enzymes except for lactate dehydrogenase were not detected in these vesicles. These results suggest the presence of a mechanism for specific uptake of cytosolic lactate dehydrogenase in both vesicles. In this study, a new type of matrix vesicles without protease was found in the tracheal cartilage, a kind of permanent cartilage, but not in the tibia epiphyseal cartilage, which is replaced by bone tissue.

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Presence of lactate dehydrogenase-containing vesicles in an intramembranous ossifying tissue: New-born mouse calvaria

Cited by 2 publications

References 23 publications

Optimization of bone‐tissue engineering in goats

Optimization of bone‐tissue engineering in goats

A New Type of Matrix Vesicles is Found in Fetal Bovine Tracheal Cartilage

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