Comparative Study of HIF-1α- and HIF-2α-Immunopositive Neurons and Capillaries in Rat Cortex under Conditions of Tissue Hypoxia

Черток, В. М.; Невзорова, В. А.; Захарчук, Н. В.

doi:10.1007/s10517-018-4207-6

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…Erlich et al reported that beclin-1 was expressed in neurons after exposure to hypoxia 4 and 24 h in mice, which was similar to our results [33]. Additionally, it was mentioned before that nucleus HIF1α might may regulate its target genes to promote a widespread adaptive response [20]. Guo reported that HIF1α may ameliorate brain damage during hypoxia through BNIP3-dependent augmentation of autophagic cell survival and reduction in cell apoptosis in rats [34].…”

Section: Discussionsupporting

confidence: 90%

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Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens)

Zhang

Cui

et al. 2023

Folia Histochem Cytobiol.

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Introduction. As a main consumer of energy, the brain is particularly susceptible to the effects of hypoxia. However, during long-term evolution, the brain of the plateau yak developed adaptive mechanisms enabling it to maintain normal physiological conditions. Materials and methods.A total of 20 male yaks belonging to two age groups [newborns (1-6 days old; n = 10) and adults (3-5 years old; n = 10)] were obtained, and the brain tissue was fixed and processed by standard methods. RT-qPCR, ELISA and IHC assays were used to investigate the expression and localization of HIF1α, BNIP3 and beclin-1 in the hippocampus, cerebral cortex, thalamus, medulla oblongata and cerebellum of newborn and adult yak brains and to explore their potential neuroprotective role.Results. We found that the expression levels of HIF1α, BNIP3 and beclin-1 at the mRNA and protein levels varied in the different regions of yak brain, with the highest expression observed in the hippocampus, followed by the cerebral cortex, thalamus, medulla oblongata and the cerebellum. Moreover, the HIF1α, BNIP3 and beclin-1 expression were significantly higher in the newborn yaks' brains than in the adult yak.The IHC results showed that HIF1α, BNIP3 and beclin-1 were mainly distributed in the neurons of the cerebral cortex, hippocampus, thalamus, medulla oblongata and cerebellum. In particular, HIF1α accumulated in the nucleus and cytoplasm.Furthermore, the immunoreactivity of BNIP3 and beclin-1 was concentrated in the cytoplasm. Conclusions.The results indicate that the yak hippocampus and cerebral cortex may be more resistant to hypoxia than thalamus, medulla oblongata and cerebellum, and the expression of BNIP3 and beclin-1 may be regulated by HIF1α to serve a neuroprotective role in the yak's brain to adaptation to hypoxia. Additionally, the brain of adult yaks may have a higher tolerance to hypoxia than the brain of newborn yaks.

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Section: Discussionsupporting

confidence: 90%

“…It was indicated neurons were more susceptible to hypoxia than glial cells. Moreover, Chertok et al and Hashemi et al pointed out that activation of HIF1α may regulate its target genes to promote a widespread adaptive response by increasing glucose transport, glycolysis, vascular growth, and cell survival [20,21].…”

Section: Discussionmentioning

confidence: 99%

Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens)

Zhang

Cui

et al. 2023

Folia Histochem Cytobiol.

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“…The key role in this process has now been established to belong to the oxygen-sensitive transcription factor HIF-1α (Ju et al, 2016;Chertok and Kotsyuba, 2017). Researchers reported that HIF-1α are expressed in the central nervous system (CNS) of mammals (Mohindra et al, 2013;López-Hernández et al, 2012;Chertok et al, 2018). The current study reported that HIF-1α mRNA and protein were positive in all regions of yak and cattle telencephalon, which was consistent with other species.…”

Section: Discussionsupporting

confidence: 86%

Comparative study on the distribution and expression of Neuroglobin and Hypoxia-inducible factor-1α in the telencephalon of yak and cattle

Liu

Mawolo

2023

Braz. J. Biol.

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The telencephalon refers to the most highly developed and anterior part of the forebrain, consisting mainly of the cerebral hemispheres. The study determined Neuroglobin (Ngb) and Hypoxia-inducible factor (HIF-1α) expression in the telencephalon of yak and cattle, and compare the expression and distribution pattern of Ngb and HIF-1α in the two animals. Immunohistochemistry (IHC), quantitative real-time Polymerase Chain Reaction (qRT-PCR), and Western blot (WB) were employed to investigate Ngb and Hif-1α expression in the telencephalon of yak and cattle. mRNA and protein expressions of Ngb and HIF-1α showed positive in different tissues of the yak and cattle telencephalon. Ngb expression in tissues of the yak recorded higher as compare to cattle while HIF-1α expression was found higher in cattle than yak. The HIF-1α expression in some tissues of yak telencephalon was consistent with the cattle. The results documented that HIF-1α may have a direct or indirect synergistic effect on Ngb expression in the yak telencephalon to improve hypoxia adaptation. It is suggested that yak may need more Ngb expression for adaptation, but the expression of HIF-1α seems to be down-regulated during long-term adaptation, and the specific causes of this phenomenon needs to be further verified.

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“…The brain is one of the main organs of the body that strongly respond to acute hypoxia. [ 29 ] Hypoxia stimulates the expression of many genes, and 1 key protein involved is HIF-1, which is a transcription factor that regulates adaptive responses to the lack of oxygen in mammalian cells. HIF-1 consists of 2 proteins, HIF-1 alpha and HIF-1 beta.…”

Section: Roles Of Hif In Cerebral Hypoxiamentioning

confidence: 99%

Role of hypoxia-inducible factor in postoperative delirium of aged patients: A review

Shen,

Yang,

Chen

et al. 2023

Medicine

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Postoperative delirium is common, especially in older patients. Delirium is associated with prolonged hospitalization, an increased risk of postoperative complications, and significant mortality. The mechanism of postoperative delirium is not yet clear. Cerebral desaturation occurred during the maintenance period of general anesthesia and was one of the independent risk factors for postoperative delirium, especially in the elderly. Hypoxia stimulates the expression of hypoxia-inducible factor-1 (HIF-1), which controls the hypoxic response. HIF-1 may have a protective role in regulating neuron apoptosis in neonatal hypoxia-ischemia brain damage and may promote the repair and rebuilding process in the brain that was damaged by hypoxia and ischemia. HIF-1 has a neuroprotective effect during cerebral hypoxia and controls the hypoxic response by regulating multiple pathways, such as glucose metabolism, angiogenesis, erythropoiesis, and cell survival. On the other hand, anesthetics have been reported to inhibit HIF activity in older patients. So, we speculate that HIF plays an important role in the pathophysiology of postoperative delirium in the elderly. The activity of HIF is reduced by anesthetics, leading to the inhibition of brain protection in a hypoxic state. This review summarizes the possible mechanism of HIF participating in postoperative delirium in elderly patients and provides ideas for finding targets to prevent or treat postoperative delirium in elderly patients.

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Comparative Study of HIF-1α- and HIF-2α-Immunopositive Neurons and Capillaries in Rat Cortex under Conditions of Tissue Hypoxia

Cited by 6 publications

References 12 publications

Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens)

Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens)

Comparative study on the distribution and expression of Neuroglobin and Hypoxia-inducible factor-1α in the telencephalon of yak and cattle

Role of hypoxia-inducible factor in postoperative delirium of aged patients: A review

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