Stakelama pacifica gen. nov., sp. nov., a new member of the family Sphingomonadaceae isolated from the Pacific Ocean Strain JLT832 T was isolated from the surface of the Pacific Ocean at 174 u 21.709 E 20 u 34.449 N and formed a deep branch within the family Sphingomonadaceae. In this study, we characterized strain JLT832T by using a polyphasic approach. Strain JLT832T was initially isolated with seawater medium (SW) containing (per litre seawater filtered by 0.2 mm film) 1 g peptone, 1 g yeast extract, 20 mg vitamin B 12 and 1 ml of a trace element solution (ES) as described by Drews (1983). The strain was identified by growing on modified rich organic (RO) medium (Yurkov et al., 1999) Cell morphology was examined using light microscopy (BX61; Olympus) and TEM (H600; Hitachi). The Gram Abbreviations: UPLC-Q-TOF-MS, ultra-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry; MR, methyl red; VP, Voges-Proskauer.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JLT832T is EU581829.A transmission electron micrograph of negatively stained cells and polar lipid profiles of strain JLT832 T are available as supplementary material with the online version of this paper.

Two novel actinomycetes, designated DQS3-9A1T and DQS3-9A2, were isolated from a saline soil contaminated with crude oil in the Shengli Oilfield in China. On the basis of 16S rRNA gene sequence analysis, the two strains were most closely related to Mycobacterium species (92.7–94.9 % similarities), and formed a distinct lineage in the suborder Corynebacterineae. In addition, the major sugars in the cell wall, arabinose and galactose, supported the affiliation of strain DQS3-9A1T with members of the family Mycobacteriaceae. However, strain DQS3-9A1T did not contain mycolic acids and MK-8 (85.5 %) was the major menaquinone for both isolates. The major cellular fatty acids for strain DQS3-9A1T were C16 : 0 (20.5 %), 10-methyl C17 : 0 (19.3 %), 10-methyl C18 : 0 (16.1 %), summed feature 3 (11.4 %), C15 : 0 (11.3 %), C17 : 0 (5.0 %) and C17 : 1 ω8c (5.0 %). The polar lipids of strain DQS3-9A1T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unknown glucosamine-containing phospholipid. These chemotaxonomic data indicated that strain DQS3-9A1T differs from the present members of the suborder Corynebacterineae. Therefore, the creation of Amycolicicoccus subflavus gen. nov., sp. nov. is proposed, with DQS3-9A1T (=DSM 45089T=CGMCC 4.3532T) as the type strain.

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