Comparative study of chromosome aberrations induced with aphidicolin in women affected by breast cancer and cervix uterine cancer

Paz‐y‐Miño, César; Peñaherrera, Ma.Serena; Sánchez, María Eugenia; Córdova, Augusta; Gutiérrez, Sara; Ocampo, Ligia; Leone, Paola

doi:10.1016/s0165-4608(96)00216-6

Cited by 15 publications

(10 citation statements)

References 21 publications

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“…But keep in mind that this is only an indirect evidence for a role of fragile sites in cancer. In addition to this correlation, breast and cervical cancer patients showed a significantly higher expression of fragile sites when treated with aphidicolin than did control groups 49. Similar results were obtained by many others 35,50–53…”

Section: Common Fragile Sites and Cancersupporting

confidence: 85%

Common Fragile Sites and Cancer: Targeted Cloning by Insertional Mutagenesis

Bushell¹

2004

Annals of the New York Academy of Sciences

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Genetic instability is an important facet of carcinogenesis and oncogenesis, generating chromosomal variability and extensive intratumor heterogeneity. Common fragile sites are predetermined chromosomal breakage regions. Experimentally, they can be demonstrated as site-specific gaps or breaks seen on metaphase chromosomes under conditions of replicative stress. They have been known for many years as a chromosomal expression of genetic instability and have been implicated to have a causative role in cancer. However, common fragile sites still remain enigmatic intrinsic parts of human chromosomes, and the DNA sequences at most of the fragile sites have not been identified so far. The idea of genetically tagging fragile site DNA by insertional mutagenesis through an exogenous marker gene has provided a platform for the efficient targeted cloning of a substantial number of common fragile sites.

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Section: Common Fragile Sites and Cancersupporting

confidence: 85%

Common Fragile Sites and Cancer: Targeted Cloning by Insertional Mutagenesis

Bushell¹

2004

Annals of the New York Academy of Sciences

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“…Though the significance of fragile sites in the development of cancer is still to be examined, the evidences of chomosomal instability in normal cells derived from cancer patients have been accumulated [2,8,14,27]. It is important to assign the chromosomal break point frequently observed in a particular type of tumor in order to identify a specific gene which is responsible for the onset of the disease.…”

Section: Methodsmentioning

confidence: 99%

Novel Putative Fragile Sites Observed in Feline Fibroblasts Treated with Aphidicolin and Fluorodeoxyuridine.

Kubo

Matsuyama

Sato

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Fragile sites are non-randomly distributed chromosomal breaks and gaps observed in the cells cultivated under certain conditions. Feline fragile sites were analyzed using skin fibroblast strains after the treatments with aphidicolin and fluorodeoxyuridine in combination with caffeine. Three aphidicolin-induced fragile sites (A1q21, C2q13 and E1p21) as well as a folate-sensitive site (B1q14) were observed in all the 3 fibroblast strains tested for each treatment group. The loci in A1q21 and B1q14 are very close to that reported previously for peripheral blood lymphocytes and lung cells. Two chromosomal break points in C2q13 and E1p21 seem to be new fragile sites. Fifteen candidates for feline fragile sites were also assigned their locations in feline chromosomes. Both the incidence and distribution of feline fragile sites in skin fibroblasts seem to be different at least in part from those in lymphocytes. -KEY WORDS: aphidicolin, chromosomal break, feline, fluorodeoxyuridine, fragile site.J. Vet. Med. Sci. 60 (7): [809][810][811][812][813] 1998 in peripheral blood lymphocytes. For the study on feline fragile sites, however, lymphocytes are less suited, because the culture often suffer a heavy loss of lectin-activated cells by aggregation with platelets. In human, the fragile sites in skin fibroblasts treated with aphidicolin differ in both frequency and distribution as compared with those observed in lymphocytes [10,11]. In the present study, we have investigated novel fragile sites induced in feline skin fibroblasts, because the banding pattern of their chromosomes have been well-characterized [12]. MATERIALS AND METHODSFeline fibroblast strains: Small patches of skin were excised from foot pads of four healthy female domestic cats under the sterile condition. The skin fragments were minced with scissors in the Ca 2+ -and Mg 2+ -free Dulbecco's phosphate buffered saline (PBS(-)), and cell suspension obtained was inoculated into 25 cm 2 plastic culture flasks containing Eagle's minimum essential medium (Nissui) supplemented with MEM sodium pyruvate solution (GIBCO), nonessential amino acid solution (GIBCO), and 10% inactivated fetal bovine serum (Hyclone). The flasks were incubated in the fumidified atmosphere containing 5% CO 2 at 37˚C and medium was replenished periodically until the growth of the fibroblasts reached confluence.Drug treatment: Single-cell suspension was prepared from a confluent culture of fibroblasts by trypsinization and inoculated into a plastic dish containing 10 ml of the growth medium. The dish was then incubated under the condition mentioned above for 2 days. Drug treatment was carried out by the procedure described by Yunis and Soreng with slight modifications [30]. Briefly, the log-phase culture of fibroblast strains FFB2, FFB3 and FFB4 were treated with Fragile sites are non-random chromosomal breaks and gaps observed in the cells under the folate-deficient condition [25] as well as those treated with particular mutagen, carcinogen and clastogens [31]. A great number of p...

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“…The results also suggest that common fragile sites may be preferential points of breakage and that expression of these sites might be a primary contributor to chromosomal damage of the somatic genome. Recently, some investigators have pointed out that there are great personal differences in the expression of fragile sites and that these sites are more frequent in normal cells of patients with some types of neoplasms [9,[17][18][19][22][23][24][25] and sometimes in their first-degree relatives [19][20][21]24,25]. Therefore, they consider that the expression of common fragile sites could be an indicator of chromosomal instability.…”

Section: Discussionmentioning

confidence: 99%

“…Several investigations have revealed that there were great individual differences in expression of common fragile sites and that the phenomenon was more frequent in normal cells of patients with certain types of malignancies [9,15,17,18] and in first-degree relatives [19][20][21].…”

Section: Introductionmentioning

confidence: 99%