Common Fragile Sites and Cancer: Targeted Cloning by Insertional Mutagenesis

Bushell, William C.

doi:10.1196/annals.1322.002

Cited by 51 publications

(24 citation statements)

References 228 publications

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“…Positions of chromosomal bands were obtained at ftp://ftp.ncbi.nih.gov/genomes/MapView/Homo_sapiens sequence/BUILD36.3/initial_release/ideogram.gz. The list of fragile sites comes from (36), where each fragile site is associated to a chromosomal band. Positions of chromosomal bands were used to obtain positions of fragile sites.…”

Section: Methodsmentioning

confidence: 99%

Large scale chromosomal mapping of human microRNA structural clusters

Mathelier¹,

Carbone²

2013

Nucleic Acids Research

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MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes crossing sequence analysis and deep sequencing data revealed the presence of >400 structural clusters of miRNAs in the human genome. An a posteriori analysis validates predictions as bona fide miRNAs. A functional analysis of structural clusters position along the chromosomes co-localizes them with genes involved in several key cellular processes like immune systems, sensory systems, signal transduction and development. Immune systems diseases, infectious diseases and neurodegenerative diseases are characterized by genes that are especially well organized around structural clusters of miRNAs. Target genes functional analysis strongly supports a regulatory role of most predicted miRNAs and, notably, a strong involvement of predicted miRNAs in the regulation of cancer pathways. This analysis provides new fundamental insights on the genomic organization of miRNAs in human chromosomes.

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Section: Methodsmentioning

confidence: 99%

Large scale chromosomal mapping of human microRNA structural clusters

Mathelier¹,

Carbone²

2013

Nucleic Acids Research

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“…Since their discovery more than three decades ago [4], more than 120 chromosome fragile sites have been identified in human [5]. They are classified as either rare or common based on their frequency and mode of induction.…”

Section: Introductionmentioning

confidence: 99%

45S rDNA Regions Are Chromosome Fragile Sites Expressed as Gaps In Vitro on Metaphase Chromosomes of Root-Tip Meristematic Cells in Lolium spp

Huang

Yang

et al. 2008

PLoS ONE

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BackgroundIn humans, chromosome fragile sites are regions that are especially prone to forming non-staining gaps, constrictions or breaks in one or both of the chromatids on metaphase chromosomes either spontaneously or following partial inhibition of DNA synthesis and have been well identified. So far, no plant chromosome fragile sites similar to those in human chromosomes have been reported.Methods and ResultsDuring the course of cytological mapping of rDNA on ryegrass chromosomes, we found that the number of chromosomes plus chromosome fragments was often more than the expected 14 in most cells for Lolium perenne L. cv. Player by close cytological examination using a routine chromosome preparation procedure. Further fluorescent in situ hybridization (FISH) using 45S rDNA as a probe indicated that the root-tip cells having more than a 14-chromosome plus chromosome fragment count were a result of chromosome breakage or gap formation in vitro (referred to as chromosome lesions) at 45S rDNA sites, and 86% of the cells exhibited chromosome breaks or gaps and all occurred at the sites of 45S rDNA in Lolium perenne L. cv. Player, as well as in L. multiflorum Lam. cv. Top One. Chromatin depletion or decondensation occurred at various locations within the 45S rDNA regions, suggesting heterogeneity of lesions of 45S rDNA sites with respect to their position within the rDNA region.ConclusionsThe chromosome lesions observed in this study are very similar cytologically to that of fragile sites observed in human chromosomes, and thus we conclude that the high frequency of chromosome lesions in vitro in Lolium species is the result of the expression of 45S rDNA fragile sites. Possible causes for the spontaneous expression of fragile sites and their potential biological significance are discussed.

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“…The mechanisms of deterioration are principally related to the generation of Reactive Oxigen Species (ROS) and to the glycosylation of proteins; both processes narrowly relate to environmental factors. On the other hand, the loss of the capacity of proliferation and regeneration is determined genetically by the shortening of the telomeres and by the processes of cellular death (Knaposwski et al, 2002;Bushell, 2005). Both exogenous and endogenous factors can be observed in aging which support the idea that there is not a single factor causing aging but that multiple connected processes contribute to it, and it is their balance which determinates the development of aging in the individual (Warner et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Cellular aging: theories and technological influence

Mercado-Sáenz

Ruiz-Gómez

Morales-Moreno

et al. 2010

Braz. arch. biol. technol.

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The aim of this article was to review the factors that influence the aging, relationship of aging with the biological rhythms and new technologies as well as the main theories to explain the aging, and to analysis the causes of aging. The theories to explain the aging could be put into two groups: those based on a program that controlled the regression of the organism and those that postulated that the deterioration was due to mutations. It was concluded that aging was a multifactorial process. Genetic factors indicated the maximum longevity of the individual and environmental factors responsible for the real longevity of the individual. It would be necessary to guarantee from early age the conservation of a natural life rhythm

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Common Fragile Sites and Cancer: Targeted Cloning by Insertional Mutagenesis

Cited by 51 publications

References 228 publications

Large scale chromosomal mapping of human microRNA structural clusters

Large scale chromosomal mapping of human microRNA structural clusters

45S rDNA Regions Are Chromosome Fragile Sites Expressed as Gaps In Vitro on Metaphase Chromosomes of Root-Tip Meristematic Cells in Lolium spp

Cellular aging: theories and technological influence

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