COMPARATIVE STUDIES ON THE MODE OF NEUTRALIZATION REACTION OF POLIOVIRUS TYPE 2 WITH SERUM IgG AND SECRETORY IgA FROM MOTHER'S MILK AND FECAL EXTRACT

Akao, Y; Sasagawa, Akira; Shiga, Sadashi; Kono, Reisaku

doi:10.7883/yoken1952.24.135

Cited by 8 publications

(4 citation statements)

References 17 publications

(14 reference statements)

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“…Breast milk contains natural molecules that can protect children against infectious agents [Akao et al, 1971; Gindrat et al, 1972; McLean and Holmes, 1980]. Among these compounds, neutralizing maternal antibodies, lactoferrin, cytokines and lipid compounds have been suggested to play an important role in that process [Isaacs et al, 1990; Basolo et al, 1993; Newburg et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Human milk can neutralize Coxsackievirus B4 in vitro

Sané¹,

Alidjinou²,

Kacet

et al. 2013

Journal of Medical Virology

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The role of enteroviruses in type 1 diabetes has long been suspected. A lower risk of type 1 diabetes is associated with breastfeeding, which could be due to a protective effect against enteroviruses. The neutralizing activity of breast milk against CVB4, a representative of enteroviruses was investigated in this study in vitro. Breast milk was cytotoxic to Hep-2 cells up to a dilution of 1/32, whereas the aqueous fraction obtained after centrifugation was not cytotoxic; although it inhibited the cytopathic effect of CVB4 on Hep-2 cell monolayers. The anti-CVB4 neutralizing activity of aqueous fractions of breast milk from 49 donors living in Northern France and 15 donors living in Congo, where enteroviral infections are more prevalent, were determined. The levels of colostrum activity expressed as titre ranged from <2 to 32 in 36% of the donors from France whereas they were >128 in every donor from Congo. Pasteurized colostrum had a lower anti-CVB4 activity compared to fresh samples (P < 0.0001, n = 49). The treatment of colostrum samples with jacalin-coated beads that bind specifically to human IgA, showed that IgA plays a role in anti-CVB4 activity. There was no correlation between the neutralizing activities of breast milk and serum (P = 0.37, n = 25). The current study showed that the variations in anti-CVB4 activity in breast milk can be attributed to environmental and living conditions. Whether a low protective activity of breast milk against enteroviruses expose newborns to a higher risk of type 1 diabetes deserves further investigation.

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Section: Introductionmentioning

confidence: 99%

Human milk can neutralize Coxsackievirus B4 in vitro

Sané¹,

Alidjinou²,

Kacet

et al. 2013

Journal of Medical Virology

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“…Previous investigations have demonstrated the presence of antibodies directed against a number of enteropathogenic agents in human colostrum and milk, including poliovirus (2,10) and Escherichia colt (7,11). Most of these lacteal immunoglobulin are recoverable from the feces of breast-fed infants (11,16).…”

mentioning

confidence: 99%

Transfer of antirotaviral antibodies from mothers to their infants

McLean¹,

Holmes²

1980

J Clin Microbiol

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Levels of rotavirus-specific immunoglobulin G (IgG), IgA, IgM, and secretory immunoglobulin in maternal and cord serum, colostrum and milk, and infants' stools were measured by enzyme-linked immunosorbent assay in 92 mothers and their infants. Although antirotaviral IgG, IgA, and secretory immunoglobulin were present in most maternal sera, only IgG crossed the placenta. All samples of colostrum and milk tested contained antirotaviral secretory immunoglobulin and IgA except those of two women in whom IgA deficiency was subsequently described. Specific IgM and IgG were also detected in many colostral samples. Antirotaviral IgA was detected in many colostral samples. Antirotaviral IgA was detected in stools of breast-fed but not bottle-fed neonates. Apparently the human infant receives rotaviral antibodies both transplacentally and via maternal colostrum and milk.

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“…It has been well known that the administration of live attenuated oral poliovirus vaccines induces both intestinal and systemic immune responses (1,5,8,9). The local immunity which may prevent poliovirus multiplication in the intestinal tract is mediated through virus-specific immunoglobulin A (IgA) antibody (1,8,10).…”

mentioning

confidence: 99%

“…The local immunity which may prevent poliovirus multiplication in the intestinal tract is mediated through virus-specific immunoglobulin A (IgA) antibody (1,8,10). In 1966, Kawakami et al (4) studied the appearance and persistence of fecal neutralizing antibodies against polioviruses in children immunized with two doses of trivalent poliovaccines with an interval of 40 days.…”

mentioning

confidence: 99%