Comparative studies on proliferation, molecular markers and differentiation potential of mesenchymal stem cells from various tissues (adipose, bone marrow, ear skin, abdominal skin, and lung) and maintenance of multipotency during serial passages in miniature pig

Lee, Ah‐Young; Lee, Jienny; Kim, Chan-Lan; Lee, Keum Sil; Lee, SoHyun; Gu, Na-Yeon; Kim, Jeong‐Min; Lee, Byeong Chun; Koo, Ok Jae; Song, Jae‐Young; Cha, Sang‐Ho

doi:10.1016/j.rvsc.2015.03.010

Cited by 43 publications

(34 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular mechanisms involved in the regulation of MSC properties, in particular stemness features, are not completely understood . Nanog and Oct4 genes have been reported to be expressed by MSCs from different species and to play a role in preserving their self‐renewal capacity and undifferentiated state . Also Dnmt1 and p21 have been suggested to influence these properties in human MSCs, in particular they have been proposed to exert opposite functions one to the other: while increased expression of the former prevents senescence and sustains proliferation and differentiation capacity, higher levels of the latter reduce stemness markers expression and proliferative potential .…”

Section: Discussionmentioning

confidence: 99%

Murine Rankl−/− Mesenchymal Stromal Cells Display an Osteogenic Differentiation Defect Improved by a RANKL-Expressing Lentiviral Vector

Schena¹,

Menale

Caci

et al. 2017

Stem Cells

View full text Add to dashboard Cite

Autosomal recessive osteopetrosis (ARO) is a severe bone disease characterized by increased bone density due to impairment in osteoclast resorptive function or differentiation. Hematopoietic stem cell transplantation is the only available treatment; however, this therapy is not effective in RANKL-dependent ARO, since in bone this gene is mainly expressed by cells of mesenchymal origin. Of note, whether lack of RANKL production might cause a defect also in the bone marrow (BM) stromal compartment, possibly contributing to the pathology, is unknown. To verify this possibility, we generated and characterized BM mesenchymal stromal cell (BM-MSC) lines from wild type and Rankl mice, and found that Rankl BM-MSCs displayed reduced clonogenicity and osteogenic capacity. The differentiation defect was significantly improved by lentiviral transduction of Rankl BM-MSCs with a vector stably expressing human soluble RANKL (hsRANKL). Expression of Rankl receptor, Rank, on the cytoplasmic membrane of BM-MSCs pointed to the existence of an autocrine loop possibly activated by the secreted cytokine. Based on the close resemblance of RANKL-defective osteopetrosis in humans and mice, we expect that our results are also relevant for RANKL-dependent ARO patients. Data obtained in vitro after transduction with a lentiviral vector expressing hsRANKL would suggest that restoration of RANKL production might not only rescue the defective osteoclastogenesis of this ARO form, but also improve a less obvious defect in the osteoblast lineage, thus possibly achieving higher benefit for the patients, when the approach is translated to clinics. Stem Cells 2017;35:1365-1377.

show abstract

Section: Discussionmentioning

confidence: 99%

Murine Rankl−/− Mesenchymal Stromal Cells Display an Osteogenic Differentiation Defect Improved by a RANKL-Expressing Lentiviral Vector

Schena¹,

Menale

Caci

et al. 2017

Stem Cells

View full text Add to dashboard Cite

show abstract

“…7,28,29 These include comparable morphology, proliferative capacity, alkaline phosphatase activity, cell surface marker expression, metabolic pathways, biologic functions, and transcription factors. 10,23,30 pASCs have often been compared side-by-side with porcine stem cells from these other tissue sources, revealing characteristics and comparable multilineage differentiation abilities as well. 9,23,28 While knowledge of the differentiation abilities of pASCs is currently limited to a few reports, porcine bone marrow-derived stem cells (pBMSCs) have been differentiated into myocytes, 31 endothelial cells, 32 and epithelial cells.…”

Section: Multilineage Differentiation Abilitiesmentioning

confidence: 99%

“…Typical cell seeding densities range from 5000 to 7000 cells/cm 2 . 5,9,10 Dulbecco's Modified Eagle Medium (DMEM) mixed 1:1 with Ham's F-12 Nutrient Mixture and supplemented with 10% Fetal Bovine Serum has been reported as ideal for the culture of pASCs. 5,10 After 48/72hrs in culture the non-adherent haematopoietic cells are removed.…”

mentioning

confidence: 99%

Properties of porcine adipose-derived stem cells and their applications in preclinical models

Arrizabalaga

Nollert

2017

Adipocyte

View full text Add to dashboard Cite

Adipose-derived stem cells represent a reliable adult stem cell source thanks to their abundance, straightforward isolation, and broad differentiation abilities. Consequently, human adipose-derived stem cells (hASCs) have been used in vitro for several innovative cellular therapy and regenerative medicine applications. However, the translation of a novel technology from the laboratory to the clinic requires first to evaluate its safety, feasibility, and potential efficacy through preclinical studies in animals. The anatomy and physiology of pigs and humans are very similar, establishing pigs as an attractive and popular large animal model for preclinical studies. Knowledge of the properties of porcine adipose-derived stem cells (pASCs) used in preclinical studies is critical for their success. While hASCs have been extensively studied this past decade, only a handful of reports relate to pASCs. The aim of this concise review is to summarize the current findings about the isolation of pASCs, their culture, proliferation, and immunophenotype. The differentiation abilities of pASCs and their applications in porcine preclinical models will also be reported.

show abstract

“…Actually, these genes are frequently reported as markers of embryonic stem cells or iPSCs; however, there are several articles that mention the positive expression of these markers in MSCs isolated from animal species. They are widely accepted to identify stem cell populations [Siegel et al, 2013;Lee et al, 2015;Ock et al, 2016]; in fact it has been reported that expression decreases with the age of the cell culture and degree of cell differentiation [Song et al, 2011;Yoon et al, 2011]. To characterize MSCs, two antibodies referred to as MSC markers, CD44 and CD90, were tested, as well as the hematopoietic cell marker CD34 as a control [Peterbauer-Scherb et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Pérez-Serrano

González-Dávalos²,

Lozano-Flores³

et al. 2016

Cells Tissues Organs

View full text Add to dashboard Cite

Purpose: The aim of this work was to evaluate the effect of PPAR agonists on the differentiation and metabolic features of porcine mesenchymal stem cells induced to the adipogenic or myogenic lineages. Methods: Bone marrow MSCs from neonate pigs were isolated and identified by cell proliferation, cell surface markers or the gene expression of stem cells (CD44, CD90, CD105 or Oct4 and Nanog, respectively). Cells were differentiated into adipose or muscle cells and treated with the PPAR agonists; adipogenic and myogenic differentiation was promoted by adding these compounds. The expression of PPARγ (an adipose marker) and MyoD1 and MyHC (muscle markers), metabolic changes and expression levels of metabolic enzymes involved in glycolysis, lipogenesis, lipolysis and the pentose phosphate pathway were tested by qPCR. Results: MSCs from neonate pigs exhibited high proliferation and were positive for CD44, CD90 and CD105 markers and Oct4 and Nanog expression. The treatment that promoted the highest expression of PPARγ was 50 µM of conjugated linoleic acid (CLA) c9 t11 (6.44 ± 0.69-fold, p ≤ 0.0001) in the adipose differentiation, and upregulation of HX2, ACCAα, ATGL, LPL and G6DP (p ≤ 0.0001) and downregulation of PFK and ACCAβ (p ≤ 0.0001) were found. For muscle differentiation, the best treatment was 50 µM of CLA c10 t12 (59.72 ± 4.72-fold, p ≤ 0.0001), and metabolic changes were upregulation of PFK, ACCAβ, G6DP, CPT1 and PPARβ/δ (p ≤ 0.0001), but no effect was observed with HX2 and ACCAα (p ≥ 0.05). Conclusions: Our results suggest that differentiated cells exhibit a typical cell lineage metabolism and higher efficiencies both in anabolism and catabolism.

show abstract

Comparative studies on proliferation, molecular markers and differentiation potential of mesenchymal stem cells from various tissues (adipose, bone marrow, ear skin, abdominal skin, and lung) and maintenance of multipotency during serial passages in miniature pig

Cited by 43 publications

References 32 publications

Murine Rankl−/− Mesenchymal Stromal Cells Display an Osteogenic Differentiation Defect Improved by a RANKL-Expressing Lentiviral Vector

Murine Rankl−/− Mesenchymal Stromal Cells Display an Osteogenic Differentiation Defect Improved by a RANKL-Expressing Lentiviral Vector

Properties of porcine adipose-derived stem cells and their applications in preclinical models

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Contact Info

Product

Resources

About