Comparative studies of mucopolysaccharides from marine animals. I. Raja eglanteria Bosc.

THE ANTICOAGULANT ACTIVITY AND STRUCTURAL CHARACTERIZATION OF FRACTIONATED AND PURIFIED GLYCOSAMINOGLYCANS FROM VENERID CLAM MERETRIX CASTA (CHEMNITZ)

“…Since these initial observations, numerous reports have appeared demonstrating that anticoagulant GAGs are present in tissues from a variety of clams, in blood, muscle, and viscera of many species of fish, in Anticoagulant Activity and Structural Characterization of Fractionated and Purified GAGs 921 cartilage of skates and sharks, and in tissues obtained from a variety of crustaceans. [13] The matechromatic activity and disaccharides profile of fractionated and purified GAGs of M. casta was depicted in Table 1. The net yield of crude, fractionated, and purified GAGs extracted as 9.85 g=kg, 33 mg=g, and 148 mg=g, respectively.…”

“…The presences of GAGs were identified through agarose gel electrophoresis in acetate buffer following the method of Patel et al [13] The molecular masses of isolated GAGs were determined by comparing with ladder (prepared by mixing of equal concentration of heparan sulfate, hyaluronic acid, and chondroitin sulfate). Then the bands were observed under gel documentation system (Genie, India) and molecular weights were determined through the molecular marker (Sigma).…”

Chondroitin Sulfate, Hyaluronic Acid and Chitin/Chitosan Production Using Marine Waste Sources: Characteristics, Applications and Eco-Friendly Processes: A Review

“…The two first stages are mostly conducted by means of alkaline hydrolysis at high concentrations of NaOH, urea or guanidine HCl, subsequently combined with selective precipitation of GAG using cationic quaternary ammonium chemicals (as cetylpyridinium chloride), potassium thiocyanate, non-ionic detergents or alcoholic solutions [59,60], deproteinization by trichloroacetic acid and finally purification with gel filtration and/or ion-exchange and size-exclusion chromatography [62]. Unfortunately, those economically viable stages lead to unsatisfactory purity for clinical uses of CS.…”

Chondroitin sulfate: extraction, purification, microbial and chemical synthesis

“…The two first stages are mostly conducted by means of alkaline hydrolysis at high concentration of NaOH, urea, cysteine or guanidine HCl, subsequently combined with selective precipitation of GAG using cationic quaternary ammonium chemicals (e.g. cetylpyridinium chloride), non‐ionic detergents, potassium thiocyanate or alcoholic solutions, deproteinization with trichloroacetic acid and finally purification with gel filtration and/or ion‐exchange and size‐exclusion chromatography . Various alternative isolation methods have been tried to replace the classical ones in pursuit of sustainability .…”