Comparative Proteomic Analysis of Rana chensinensis Oviduct

Su, Hang; Zhang, He; Wei, Xinghua; Pan, Daian; Li, Jing; Zhao, Daqing; Zhao, Yu; Qi, Bin

doi:10.3390/molecules23061384

Cited by 11 publications

(8 citation statements)

References 68 publications

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“…7 Su et al found that swelling capacity was mainly related to extracellular matrix (ECM) and focal adhesion compared to the protein expression profiles of Rana chensinensis oviduct during the breeding period and prehibernation. 25 Mucins belong to a family of heavily glycosylated proteins with high molecular weight, secreted by mucosal epithelium.…”

Section: Swelling Capacity-related Differentially Expressed Proteins In Ormentioning

confidence: 99%

Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis

Zhang¹,

Zhang²,

Yang³

et al. 2021

IJPER

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Introduction: Oviductus Ranae (OR) is the dried oviduct of female Rana temporaria chensinensis David, which is one of the best-known and highly valued oriental foods and medicines in China. OR has a unique physiological phenomenon that is high swelling capacity. Objectives: We aimed to study which components are associated with high swelling capacity in OR. Materials and Methods: isobaric tags for relative and absolute quantification (iTRAQ) proteomic methods were used to identify differentially expressed proteins between the OR and three counterfeit oviducts, including Rana nigromaculata, Bufo bufogargarizans and Rana catesbeiana to explore the mechanism of swelling capacity. Results: We found that a total of 1220 proteins were identified from 2149 unique peptide sequences. Comparing with three counterfeit oviducts, 11 differentially expressed proteins were identified in OR, including 9 up-regulated proteins, such as mucins (MUC2, MUC5AC, MUC5AC-like, MUC5B), PREDICTED: a-kinase anchor protein 13-like (AKAP13-like), PREDICTED: IgGFc-binding protein (FcγBP), PREDICTED: IgGFcbinding protein-like (FcγBP-like), et al. and 2 down-regulated proteins. Bioinformatics results found that these up-regulated proteins were involved in biological processes, including metabolic process, cellular process, responses to stimulus and biological regulation. Conclusion: Mucins, AKAP13-like, FcγBP and FcγBP-like may act as a positive regulator for the innate immune response in oviduct of Chinese brown frog and exceptional swelling capacity of OR is closely related to gel-forming mucins. Taken together, our results provide the basis for further understanding of the unique physiological phenomenon of OR.

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Section: Swelling Capacity-related Differentially Expressed Proteins In Ormentioning

confidence: 99%

Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis

Zhang¹,

Zhang²,

Yang³

et al. 2021

IJPER

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“…Rana chensinensis ( R. chensinensis ) is an important wild animal in China. Oviductus Ranae, a valuable Chinese crude drug, is recorded in Pharmacopoeia of the People’s Republic of China as a dried oviduct of the female Chinese frog [9], R. chensinensis , distributed mainly in northeastern China. Oviductus Ranae is an established and highly valued food and medicine.…”

Section: Introductionmentioning

confidence: 99%

DE NOVOSEQUENCING AND ANALYSIS OF THERANA CHENSINENSISTRANSCRIPTOME TO DISCOVER PUTATIVE GENES ASSOCIATED WITH POLYUNSATURATED FATTY ACIDS

Sun

Wang

Zhang

2020

Preprint

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32Rana chensinensis (R. chensinensis) is an important wild animal found in China, and 33 a precious animal in Chinese herbal medicine. R. chensinensis is rich in 34 polyunsaturated fatty acids (PUFAS). However, information regarding the genes of R. 35 chensinensis related to the synthesis of PUFAs is limited. To identify these genes, we 36 performed Illumina sequencing of R. chensinensis RNA from the skin and Oviductus 37 Ranae. The Illumina Hiseq 2000 platform was used for sequencing, and the I-Sanger 38 cloud platform was used for transcriptome de novo sequencing and information 39 analysis to generate a database. Through the database generated by the transcriptome 40 and the pathway map, we found the pathway for the biosynthesis of R. chensinensis 41 PUFAs. The Pearson coefficient method was used to analyze the correlation of gene 42 expression levels between samples, and the similarity of gene expression in different 43 tissues and the characteristics in their respective tissues were found. Twelve 44 differentially expressed genes of PUFA in skin and Oviductus Ranae were screened 45 by gene differential expression analysis. The 12 unigenes expression levels of 46 qRT-PCR were used to verify the results of gene expression levels consistent with 47 transcriptome analysis. Based on the sequencing, key genes involved in biosynthesis 48 of unsaturated fatty acids were isolated, which established a biotechnological platform 49 for further research on R. chensinensis. 50 51

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“…Furthermore it can also provide a fine-tuned immune system to get over potential pathogens. [3][4][5] In female genital tract, there are a large number of microorganisms that may cause disease such as inflammation of the oviduct and its immune system has the ability of dealing with pathogenic microorganism. 6 For example, some report has shown that TLR4 is expressed in different types of uterine and oviductal cells of rabbit and their levels are increased by lipopolysaccharide (LPS) administration suggesting that their involvement in innate immunity for host defense was also found in the rabbit.…”

Section: Introductionmentioning

confidence: 99%

“…16,17 The hibernation period of Rana dybowskii ranges from October to February of the following year, and then the breeding period ranges from February to June dependent on the latitude. 4 One special physiological phenomenon of Rana dybowskii is that its oviducts goes through expansion prior to hibernation instead of expanding during the breeding period. The dry product of expanded oviduct called rana oil has been widely used in traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Zhang

Zhao

et al. 2019

Eur J Histochem

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One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation.

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Comparative Proteomic Analysis of Rana chensinensis Oviduct

Cited by 11 publications

References 68 publications

Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis

Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis

DE NOVOSEQUENCING AND ANALYSIS OF THERANA CHENSINENSISTRANSCRIPTOME TO DISCOVER PUTATIVE GENES ASSOCIATED WITH POLYUNSATURATED FATTY ACIDS

Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

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