DE NOVOSEQUENCING AND ANALYSIS OF THERANA CHENSINENSISTRANSCRIPTOME TO DISCOVER PUTATIVE GENES ASSOCIATED WITH POLYUNSATURATED FATTY ACIDS

Abstract

32Rana chensinensis (R. chensinensis) is an important wild animal found in China, and 33 a precious animal in Chinese herbal medicine. R. chensinensis is rich in 34 polyunsaturated fatty acids (PUFAS). However, information regarding the genes of R. 35 chensinensis related to the synthesis of PUFAs is limited. To identify these genes, we 36 performed Illumina sequencing of R. chensinensis RNA from the skin and Oviductus 37 Ranae. The Illumina Hiseq 2000 platform was used for sequencing, and the I-Sanger 38 cloud platform was used for transcriptome de novo sequencing and information 39 analysis to generate a database. Through the database generated by the transcriptome 40 and the pathway map, we found the pathway for the biosynthesis of R. chensinensis 41 PUFAs. The Pearson coefficient method was used to analyze the correlation of gene 42 expression levels between samples, and the similarity of gene expression in different 43 tissues and the characteristics in their respective tissues were found. Twelve 44 differentially expressed genes of PUFA in skin and Oviductus Ranae were screened 45 by gene differential expression analysis. The 12 unigenes expression levels of 46 qRT-PCR were used to verify the results of gene expression levels consistent with 47 transcriptome analysis. Based on the sequencing, key genes involved in biosynthesis 48 of unsaturated fatty acids were isolated, which established a biotechnological platform 49 for further research on R. chensinensis. 50 51