Comparative proteomic analysis of plasma from bipolar depression and depressive disorder: identification of proteins associated with immune regulatory

Chen, Jin; Huang, Cheng‐Long; Song, Y.R.; Shi, Haiyang; Wu, Dongrui; Yang, Yongtao; Rao, Chenglong; Liao, Lina; Wu, You; Tang, Juliet D.; Cheng, Ke; Zhou, Jian; Xie, Peng

doi:10.1007/s13238-015-0218-5

Cited by 22 publications

(52 citation statements)

References 17 publications

(16 reference statements)

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“…ROCK2 was also overexpressed in MDD compared to in BD. The direction of expression and the protein itself were consistent with the results found by Chen et al [20]. ROCK is a serine/threonine kinase that is a crucial regulator of the actin cytoskeleton and cell polarity [41].…”

Section: Discussionsupporting

confidence: 89%

“…Chen et al (2015) compared plasma samples from drug-naïve patients and identified 25 DEPs, whereas Ren et al (2017) compared plasma samples from drug-free patients and identified nine DEPs [19,20]. However, no proteins were common between studies [19,20]. We compared serum samples from subjects with MDD and BD and found only one protein, Rho-associated protein kinase 2 (ROCK2), as a duplicate protein from the previous studies [20].…”

Section: Discussionmentioning

confidence: 92%

“…However, no proteins were common between studies [19,20]. We compared serum samples from subjects with MDD and BD and found only one protein, Rho-associated protein kinase 2 (ROCK2), as a duplicate protein from the previous studies [20]. These discrepancies are likely related to variations in the study designs and differences in the demographic and clinical characteristics of the study.…”

Section: Discussionmentioning

confidence: 99%

“…To date, there are two published articles directly comparing DEPs between MDD and BD [19,20]. Chen et al (2015) compared plasma samples from drug-naïve patients and identified 25 DEPs, whereas Ren et al (2017) compared plasma samples from drug-free patients and identified nine DEPs [19,20]. However, no proteins were common between studies [19,20].…”

Section: Discussionmentioning

confidence: 99%

“…Different articles have reported quantitative protein profile differences between MDD or BD and controls [12,[15][16][17][18]. However, few studies have compared the profiles of MDD and BD [19,20]. Thus, this study was conducted to compare the peripheral proteomic profiles between MDD and BD.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of serum protein profiles between major depressive disorder and bipolar disorder

et al. 2020

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Background: Major depressive disorder and bipolar disorder are prevalent and debilitating psychiatric disorders that are difficult to distinguish, as their diagnosis is based on behavioural observations and subjective symptoms. Quantitative protein profile analysis might help to objectively distinguish between these disorders and increase our understanding of their pathophysiology. Thus, this study was conducted to compare the peripheral protein profiles between the two disorders. Methods: Serum samples were collected from 18 subjects with major depressive disorder and 15 subjects with bipolar disorder. After depleting abundant proteins, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and label-free quantification were performed. Data-dependent acquisition data were statistically analysed from the samples of 15 subjects with major depressive disorder and 10 subjects with bipolar disorder who were psychotropic drug-free. Two-sided t-tests were performed for pairwise comparisons of proteomes to detect differentially-expressed proteins (DEPs). Ingenuity Pathway Analysis of canonical pathways, disease and functions, and protein networks based on these DEPs was further conducted. Results: Fourteen DEPs were significant between subjects with major depressive disorder and those with bipolar disorder. Ras-related protein Rab-7a (t = 5.975, p = 4.3 × 10 − 6) and Rho-associated protein kinase 2 (t = 4.782, p = 8.0 × 10 − 5) were significantly overexpressed in subjects with major depressive disorder and Exportin-7 (t =-4.520, p = 1.5 × 10 − 4) was significantly overexpressed in subjects with bipolar disorder after considering multiple comparisons. Bioinformatics analysis showed that cellular functions and inflammation/immune pathways were significantly different. Conclusions: Ras-related protein Rab-7a, Rho-associated protein kinase 2, and Exportin-7 were identified as potential peripheral protein candidates to distinguish major depressive disorder and bipolar disorder. Further large sample studies with longitudinal designs and validation processes are warranted.

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Section: Discussionsupporting

confidence: 89%