Comparative proteomic analysis of Bactrocera dorsalis (Hendel) in response to thermal stress

Wei, Dong; Fu, Jia; Tian, Chuan-Bei; Tian, Yi; Smagghe, Guy; Dou, Wei; Wang, Jinjun

doi:10.1016/j.jinsphys.2015.01.012

Cited by 21 publications

(16 citation statements)

References 51 publications

(55 reference statements)

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“…Previous reports suggested that many enzymes in fatty acid metabolism showed upregulated expression, which is widely regarded as an important energy budget strategy in the HS environment (Sun et al., ; Thorne, Burns, Fraser, Hillyard & Clark, ). Cells could also protect against damage under heat stress by regulation of amino acids (AA) metabolism, such as enzymes in glutathione (GSH) metabolic pathway (Gonzalezesquerra & Leeson, ; Maher, ; Wei et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Zhou

Yang

2017

Aquac Res

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The sea cucumber Apostichopus japonicus is an economic species mainly distributed along the coast of northern China, south‐eastern Russia, Japan, Republic of Korea and Democratic People's Republic of Korea. Aquaculture industry of A. japonicus has been facing severe challenge of high temperature. In this study, we studied the mRNA expression profiles of eight key metabolic enzymes involved in carbohydrate and amino acids metabolism in A. japonicus under heat stress. The expression of hexokinase, pyruvate kinase and malate dehydrogenase showed downregulated response to heat stress, while the expression of glutamate dehydrogenase, alanine transaminase and branched‐chain aminotransferase showed upregulated response. In addition, the expression of lactate dehydrogenase and glutamate synthase showed no significant difference. We also applied 1H NMR‐based metabolomics to investigate metabolic changes in the intestine tissue of A. japonicus under heat stress, the results of which revealed nine increased and 10 decreased metabolites in the heat stress group. These response genes and metabolites have potential to become markers for identifying severity of heat stress. More importantly, our findings suggest significant links between gene expression and metabolites changing, highlighting regulation networks of carbohydrate and amino acid metabolism in heat‐stressed A. japonicus.

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Section: Introductionmentioning

confidence: 99%

“…. Cells could also protect against damage under heat stress by regulation of amino acids (AA) metabolism, such as enzymes in glutathione (GSH) metabolic pathway (Gonzalezesquerra & Leeson, 2006;Maher, 2005;Wei et al, 2015).…”

mentioning

confidence: 99%

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Zhou

Yang

2017

Aquac Res

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“…through physiological adaptation within the lifetime of an individual, is one alternative way to cope with extreme and fluctuating temperatures (Angiletta, 2009;Chown and Terblanche, 2006;Hoffmann and Parsons, 1997;Hoffmann et al, 2003). Studies on ectotherms investigating thermal acclimation and the physiological changes induced in response to acclimation typically do so by exposing adult organisms to short-term cold or hot temperatures and subsequently studying the consequences on thermal and molecular phenotypes (Overgaard et al, 2005;Sørensen et al, 2005;Malmendal et al, 2006;Teranishi and Stillman, 2007;Nota et al, 2010;Teets et al, 2012;Colinet et al, 2013;Jansen et al, 2013;Wei et al, 2015). Such studies have provided novel insights into the molecular aspects of acclimation responses and have added important components to our knowledge on associations between the genotype, the molecular phenotype and thermal resistance.…”

Section: Introductionmentioning

confidence: 99%

“…Since then, technologies have been developing fast and state of the art methods now enable identification and quantification of sometimes thousands of proteins in organisms such as Escherichia coli (Soufi et al, 2015). In spite of this, studies on thermal acclimation responses in arthropods have rarely identified more than a few hundred proteins (Colinet et al, 2007(Colinet et al, , 2013Pedersen et al, 2010;Wei et al, 2015), making investigation of complex physiological changes in response to thermal acclimation difficult.…”

Section: Introductionmentioning

confidence: 99%

Proteomic data reveals a physiological basis for costs and benefits associated with thermal acclimation

Kristensen

Kjeldal

Schou

et al. 2016

Journal of Experimental Biology

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Physiological adaptation through acclimation is one way to cope with temperature changes. Biochemical studies on acclimation responses in ectotherms have so far mainly investigated consequences of short-term acclimation at the adult stage and focussed on adaptive responses. Here, we assessed the consequences of rearing Drosophila melanogaster at low (12°C), benign (25°C) and high (31°C) temperatures. We assessed cold and heat tolerance and obtained detailed proteomic profiles of flies from the three temperatures. The proteomic profiles provided a holistic understanding of the underlying biology associated with both adaptive and non-adaptive temperature responses. Results show strong benefits and costs across tolerances: rearing at low temperature increased adult cold tolerance and decreased adult heat tolerance and vice versa with development at high temperatures. In the proteomic analysis, we were able to identify and quantify a large number of proteins compared with previous studies on ectotherms (1440 proteins across all replicates and rearing regimes), enabling us to extend the proteomic approach using enrichment analyses. This gave us detailed information on individual proteins, as well as pathways affected by rearing temperature, pinpointing potential mechanisms responsible for the strong costs and benefits of rearing temperature on functional phenotypes. Several well-known heat shock proteins, as well as proteins not previously associated with thermal stress, were among the differentially expressed proteins. Upregulation of proteasome proteins was found to be an important adaptive process at high-stress rearing temperatures, and occurs at the expense of downregulation of basal metabolic functions.

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“…2012; Zhao and Jones 2012; Wei et al. 2015). P. pseudoannulata might therefore possess a series of gene regulatory and metabolic response mechanisms to deal with thermal stress.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Xiao

Wang

Cao

et al. 2016

Ecology and Evolution

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The wolf spider Pardosa pseudoannulata is a dominant predator in paddy ecosystem and an important biological control agent of rice pests. Temperature represents a primary factor influencing its biology and behavior, although the underlying molecular mechanisms remain unknown. To understand the response of P. pseudoannulata to temperature stress, we performed comparative transcriptome analyses of spider adults exposed to 10°C and 40°C for 12 h. We obtained 67,725 assembled unigenes, 21,765 of which were annotated in P. pseudoannulata transcriptome libraries, and identified 905 and 834 genes significantly up‐ or down‐regulated by temperature stress. Functional categorization revealed the differential regulation of transcription, signal transduction, and metabolism processes. Calcium signaling pathway and metabolic pathway involving respiratory chain components played important roles in adapting to low temperature, whereas at high temperature, oxidative phosphorylation and amino acid metabolism were critical. Differentially expressed ribosomal protein genes contributed to temperature stress adaptation, and heat shock genes were significantly up‐regulated. This study represents the first report of transcriptome identification related to the Araneae species in response to temperature stress. These results will greatly facilitate our understanding of the physiological and biochemical mechanisms of spiders in response to temperature stress.

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Comparative proteomic analysis of Bactrocera dorsalis (Hendel) in response to thermal stress

Cited by 21 publications

References 51 publications

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Proteomic data reveals a physiological basis for costs and benefits associated with thermal acclimation

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

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