Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber<i>Apostichopus japonicus</i>

Xu, Dongxue; Zhou, Shun; Yang, Hongsheng

doi:10.1111/are.13411

Cited by 34 publications

(6 citation statements)

References 47 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Valine is a branched-chain AA; valine catabolism begins with the elimination of the amino group by transamination, giving alpha-ketoisovalerate, an alpha-keto acid, which is then transformed to isobutyryl-CoA through oxidative decarboxylation by the branched-chain α -ketoacid dehydrogenase complex [ 27 ]. Similar to the present study, Xu et al [ 28 ] observed that the valine concentration was increased in Apostichopus japonicus during short-term acute HS. We argue that valine is a potential marker for short-term HS.…”

Section: Discussionsupporting

confidence: 91%

Effects of recovery from short-term heat stress exposure on feed intake, plasma amino acid profiles, and metabolites in growing pigs

Kim¹,

Reddy²,

Kim³

et al. 2021

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 91%

Effects of recovery from short-term heat stress exposure on feed intake, plasma amino acid profiles, and metabolites in growing pigs

Kim¹,

Reddy²,

Kim³

et al. 2021

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

“…www.nature.com/scientificreports www.nature.com/scientificreports/ As reported in previous studies, abalones experienced elevated ammonia excretion rate and weight loss when reared under high temperature 11,24 , suggesting the increased contribution of protein/amino acid oxidation to their energy supply. The metabolomics-based studies on small abalone H. diversicolor 43 , sea cucumber Apostichopus japonicushas 38,44 also showed the influence of heat stress on amino acid metabolism. In this study, tissue concentrations of two tryptophan metabolic intermediates significantly increased after heat stress (Table 1): L-kynurenine and indole-3-lactic acid (3-IAA).…”

Section: Discussionmentioning

confidence: 94%

Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Gao

Liu

2020

Sci Rep

View full text Add to dashboard Cite

Temperature fluctuation is a key abiotic factor for the growth and survival of Pacific abalone Haliotis discus hannai, particularly during climate change. However, the physiological mechanism underlying the abalones' response to heat stress remains unknown. We sought to understand the metabolic adaptation mechanism of Pacific abalone to heat stress for further analyzing its heat tolerance capacity. For two groups experienced different acclimate temperature (10 °C and 30 °C for 62 days), the Pacific abalone juveniles displayed significantly different survival rates under 31 °C acute heat treatment. A total of 1815 and 1314 differential metabolites were identified from the 10 °C and 30 °C acclimate groups respectively, by comparing mass spectrometry data of the samples before and after heat stimulation. Heat stress led to mitochondrial failure, resulting in incomplete oxidative metabolism of amino acids and fatty acids in the mitochondria, and massive accumulation of unstable metabolic intermediates in cells. The 10 °C acclimated group accumulated more harmful substances after heat stimulation, provoking further stress responses and pathophysiological processes. In comparison, the 30 °C acclimated group showed stronger regulation capacity to produce beneficial substances for metabolic homeostasis. The findings provided insight into the heat response of marine animals, especially concerning mitochondrial metabolism. Global warming will trigger more extreme weather events that can potentially be deleterious to marine animals, especially the ones distributed in the intertidal and neritic zones 1,2. Organisms inhabiting this range face higher water temperature elevations and more environmental fluctuations during the summer season. Aquaculture animals, which are subjected to fluctuations in natural conditions, are more vulnerable to these physical and chemical stimulations as they are usually stocked intensively at high density and rely on water influx from coastal areas 3. Even a slight change in the climate may cause enormous economic losses in mariculture. The analysis of thermal stress on aquaculture animals has thus gained more research interests 4-7. Pacific abalone Haliotis discus hannai, a cold water gastropod, is the predominantly farmed abalone species in many Asian countries 8. It is naturally distributed in cold water, implying that high temperatures would limit its southern expansion along the Asian coast. In recent years, the dominant aquaculture strategy in China is transferring juvenile abalones to warmer south China during the winter, thereby accelerating the animal's growth rate. In the meantime, the traditional genetic selection based on growth traits may contribute to a decreased heat stress tolerance of the organism. Consequently, the massive mortality in summer months has become a challenge that has led to an enormous economic loss in abalone and the entire molluscs industry in recent years 9. While some overwintering abalones were transferred back to the northern region during spring, some were c...

show abstract

“…Acclimation in tanks lasted for 2 weeks. During both the acclimation and treatment experiment, the sea cucumbers were fed with regular fodder, and half of the water was changed daily [47].…”

Section: Methodsmentioning

confidence: 99%

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Wang

2019

IJMS

Self Cite

View full text Add to dashboard Cite

Heat stress (HS) is an important factor for the survival of the marine organism Apostichopus japonicus. Lysine acetylation is a pivotal post-translational modification that modulates diverse physiological processes including heat shock response (HSR). In this study, 4028 lysine acetylation sites in 1439 proteins were identified in A. japonicus by acetylproteome sequencing. A total of 13 motifs were characterized around the acetylated lysine sites. Gene Ontology analysis showed that major acetylated protein groups were involved in “oxidation–reduction process”, “ribosome”, and “protein binding” terms. Compared to the control group, the acetylation quantitation of 25 and 41 lysine sites changed after 6 and 48 h HS. Notably, lysine acetyltransferase CREB-binding protein (CBP) was identified to have differential acetylation quantitation at multiple lysine sites under HS. Various chaperones, such as caseinolytic peptidase B protein homolog (CLBP), T-complex protein 1 (TCP1), and cyclophilin A (CYP1), showed differential acetylation quantitation after 48 h HS. Additionally, many translation-associated proteins, such as ribosomal proteins, translation initiation factor (IF), and elongation factors (EFs), had differential acetylation quantitation under HS. These proteins represented specific interaction networks. Collectively, our results offer novel insight into the complex HSR in A. japonicus and provide a resource for further mechanistic studies examining the regulation of protein function by lysine acetylation.

show abstract

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Cited by 34 publications

References 47 publications

Effects of recovery from short-term heat stress exposure on feed intake, plasma amino acid profiles, and metabolites in growing pigs

Effects of recovery from short-term heat stress exposure on feed intake, plasma amino acid profiles, and metabolites in growing pigs

Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Contact Info

Product

Resources

About