Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Abstract

The wolf spider Pardosa pseudoannulata is a dominant predator in paddy ecosystem and an important biological control agent of rice pests. Temperature represents a primary factor influencing its biology and behavior, although the underlying molecular mechanisms remain unknown. To understand the response of P. pseudoannulata to temperature stress, we performed comparative transcriptome analyses of spider adults exposed to 10°C and 40°C for 12 h. We obtained 67,725 assembled unigenes, 21,765 of which were annotated in P. pseudoannulata transcriptome libraries, and identified 905 and 834 genes significantly up‐ or down‐regulated by temperature stress. Functional categorization revealed the differential regulation of transcription, signal transduction, and metabolism processes. Calcium signaling pathway and metabolic pathway involving respiratory chain components played important roles in adapting to low temperature, whereas at high temperature, oxidative phosphorylation and amino acid metabolism were critical. Differentially expressed ribosomal protein genes contributed to temperature stress adaptation, and heat shock genes were significantly up‐regulated. This study represents the first report of transcriptome identification related to the Araneae species in response to temperature stress. These results will greatly facilitate our understanding of the physiological and biochemical mechanisms of spiders in response to temperature stress.

Developmental plasticity in reptiles: Insights from temperature‐dependent gene expression in wall lizard embryos

“…This suggests that the rate of translation of mRNA into protein is affected by temperature, possibly counter‐acting decreased transcriptional rates. Differential expression of ribosomal proteins and RNAs have been observed in a wide variety of animals in response to both cold (Long et al., ; Xiao, Wang, Cao, & Zhang, ) and warm (Liu et al., ; Prado‐Lima & Val, ; Quinn, McGowan, Cooper, Koop, & Davidson, ) temperatures, yet only one study (Long et al., ) provides indirect evidence that they may increase resistance to temperature stress. In that study, zebrafish larvae acclimated to cold temperatures for 24 hr show both increased expression of ribosomal genes and increased survival to acute cold stress.…”

“…RNAs have been observed in a wide variety of animals in response to both cold (Long et al, 2012;Xiao, Wang, Cao, & Zhang, 2016) and…”

Molecular characterization and expression of vitellogenin genes from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

“…Triplicate reactions were performed for each sample and three biological replicates were tested. The expression of β-actin gene was used as an internal control as previously described (Xiao et al, 2016). The relative expression level of PpVg was calculated according to the 2 −ΔΔCt method (Livak & Schmittgen, 2001).…”

Transcriptomic response of wolf spider, Pardosa pseudoannulata, to transgenic rice expressing Bacillus thuringiensis Cry1Ab protein

“…Meng et al sequenced cephalothoraxes of P. pseudoannulata adults and identified genes involved in insecticide metabolism and detoxification, including P450s, GSTs, AChEs, AChRs, GABA receptors, and GluCI [41]. Xiao et al carried out RNA-Seq analysis in P. pseudoannulata and revealed an array of genes responding to temperature stress [42]. In this study, we focused on the genes corresponding to ingested Bt toxins.…”