Comparative portrayal of ocular surface microbe with and without dry eye

Li, Zhenhao; Gong, Yufang; Chen, ShuZe; Li, Siqi; Zhang, Yu; Huang, Zhong; Wang, ZhouCheng; Chen, Yifan; Deng, Qixin; Jiang, Yuting; Li, Liying; Fu, Min; Yi, Guoguo

doi:10.1007/s12275-019-9127-2

Cited by 53 publications

(43 citation statements)

References 36 publications

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“…2B and Fig. 3) (Dong et al 2011; Lee et al 2012; Doan et al 2016; Huang et al 2016; Ozkan et al 2017; Zhang et al 2017; Ham et al 2018; Cavuoto et al, 2018a, 2018b; Dong et al 2019; Ge et al 2019; Li et al 2019a; Li et al 2019b). We were not able to include the papers of Shin et al ( n = 11) (no raw data available) (Shin et al 2016) and Zhou et al ( n = 105) (raw data available but both paediatric and adult merged) (Zhou et al 2014).…”

Section: Resultsmentioning

confidence: 98%

“…Amplicon sequencing results of healthy volunteers at phylum level. A, Underaged population (<18 years old (yo)) (Butcher et al 2017; Cavuoto et al 2018; Yau et al 2019) versus adults (>18 yo) (Dong et al 2011; Lee et al 2012; Doan et al 2016; Huang et al 2016; Ozkan et al 2017; Zhang et al 2017; Ham et al 2018; Cavuoto et al 2018; Dong et al 2019; Ge et al 2019; Li et al 2019; Li et al 2019). Proteobacteria and Actinobacteria are the most prominent phyla.…”

Section: Resultsmentioning

confidence: 99%

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Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data

Delbeke

Younas

Casteels

et al. 2020

Acta Ophthalmologica

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Insights in the ocular surface microbiome are still at an early stage and many more questions remain unanswered compared with other human‐associated microbial communities. The current knowledge on the human microbiome changed our viewpoint on bacteria and human health and significantly enhanced our understanding of human pathophysiology. Also in ocular medicine, microbiome research might impact treatment. Here, we summarize the current knowledge on ocular microbiome research with a particular focus on potential confounding factors and their effects on microbiome composition. Moreover, we present the ocular surface core microbiome based on current available data and defined it as genera present in almost half of the published control cohorts with a relative abundance of at least 1%.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data

Delbeke

Younas

Casteels

et al. 2020

Acta Ophthalmologica

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“…Whether this observation is specific to the mouse needs to be confirmed in human patients, and might support in-depth exploration of the role of the ocular surface microbiome in DED and meibomian gland dysfunctions. [32][33][34] In the cornea, in addition to the pathways present in the lacrimal gland and conjunctiva, two additional pathways: transcriptional regulation and the NLR signaling pathways, reached the top five position (with seven genes modulated in each pathway). It is important to note that all the genes identified are present in more than one pathway (see supplementary tables S1 to S3).…”

Section: Exploration Of the Signaling Pathways Modulated By The Ded Ementioning

confidence: 99%

Ocular surface response of two preservative-free cylcosporine A emulsion eye drops in a mouse model of dry eye

Daull

Nagano

Gros³

et al. 2021

Current Eye Research

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Purpose/Aim: Dry eye (DE) disease is a multifactorial disease in which uncontrolled inflammation can lead to corneal epithelium lesions and symptoms of discomfort. The aim of the present study was to evaluate the efficacy of two cyclosporine emulsions in a mouse model of DE with corneal epithelium lesions. Materials and Methods: Six-to 9-week-old female C57BL/6 N mice were housed in a controlledenvironment room to induce DE. Following DE development, mice were instilled with: QD 0.1%CsA cationic emulsion (CaEm), BID 0.05%CsA anionic emulsion (AEm), or left untreated. Aqueous tear production and corneal epithelium lesions were assessed throughout the experiment. At the end of the treatment period, left eyes were sampled, fixed, and stained for histology, while the cornea, conjunctiva, and lacrimal gland of right eyes were sampled for transcriptomic analysis. Results: Corneal lesion scores were reduced by 10.4%, 18.4%, and 10.9% at day 6, 10, and 14, respectively, with CaEm (QD), and by 2.6%, 3.0%, and 5.5% at day 6, 10, and 14, respectively, with AEm (BID). Histology demonstrated that 7 out of 10 DE mice presented moderate to severe ocular lesions, while only 2 and 5 out of 10 mice presented slight to moderate ocular lesions when treated with the CaEm (QD) and AEm (BID), respectively. The transcriptomic profile analysis suggests that a different set of inflammatory genes are modulated in the cornea, conjunctiva, and lacrimal gland upon DE development. In addition, the two emulsions distinctively modulate the gene expression profile. Conclusions: This study demonstrates that both emulsions were effective at reducing corneal lesions, with the CaEm (QD) being slightly better than the AEm (BID). Interestingly, this study suggests that ocular tissues may not respond similarly to a dry environment and that a different set of genes is modulated by the two formulations in the ocular tissues.

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“…The metagenomic data collected from our laboratory revealed that Propionibacterium , Staphylococcus , Escherichia , and Micrococcus were the most abundant ocular surface microbial genera in healthy humans ( 33 ). Li et al found the predominant genera to be Pseudomonas , Acinetobacter , Bacillus , Chryseobacterium , and Corynebacterium ( 34 ). The more recent study carried out by Suzuki et al demonstrated that the ocular surface was typically dominated by Propionibacterium in the young subjects and by Corynebacterium or Neisseriaceae in the elderly subjects ( 35 ).…”

Section: Defining the Ocular Microbiotamentioning

confidence: 99%

“…Meibomian gland dysfunction often leads to evaporative dry eye syndrome. Inconsistencies in the microbial species that changed by dry eye syndrome and meibomian gland dysfunction remain in several studies ( 34 , 42 , 43 ). This may again be explained by sample size, sequencing approach, and different diagnostic criteria.…”

Section: Factors Changing the Ocular Microbiotamentioning

confidence: 99%

Ocular Microbiota and Intraocular Inflammation

Wei

2020

Front. Immunol.

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The term ocular microbiota refers to all types of commensal and pathogenic microorganisms present on or in the eye. The ocular surface is continuously exposed to the environment and harbors various commensals. Commensal microbes have been demonstrated to regulate host metabolism, development of immune system, and host defense against pathogen invasion. An unbalanced microbiota could lead to pathogenic microbial overgrowth and cause local or systemic inflammation. The specific antigens that irritate the deleterious immune responses in various inflammatory eye diseases remain obscure, while recent evidence implies a microbial etiology of these illnesses. The purpose of this review is to provide an overview of the literature on ocular microbiota and the role of commensal microbes in several eye diseases. In addition, this review will also discuss the interaction between microbial pathogens and host factors involved in intraocular inflammation, and evaluate therapeutic potential of targeting ocular microbiota to treat intraocular inflammation.

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Comparative portrayal of ocular surface microbe with and without dry eye

Cited by 53 publications

References 36 publications

Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data

Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data

Ocular surface response of two preservative-free cylcosporine A emulsion eye drops in a mouse model of dry eye

Ocular Microbiota and Intraocular Inflammation

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