Comparative pharmacokinetics study of six effective components between two dosage forms of Qixue–Shuangbu Prescription in rats by UPLC–MS/MS

Abstract

Qixue-Shuangbu Prescription (QSP) is an efficacious prescription for treating heart failure, myocardial ischemia and other diseases. It is composed of nine Chinese herbs.This study investigated and compared the pharmacokinetics of QSP in rats by UPLC-MS/MS between two dosage forms of traditional decoction (TD) and compound tincture (CT). Owing to the complexity of the chemicals in QSP, ginsenoside Rg1, ginsenoside Re, ferulic acid, astragaloside IV, rhein and calycosin were chosen for the pharmacokinetics study. The method established for detecting serum specimens was shown to have acceptable selectivity, linearity, lower limit of quantitation, precision, accuracy, recovery, matrix effect and stability. The peak concentration, AUC 0-t and AUC 0-∞ of ginsenoside Re, ginsenoside Rg1, ferulic acid and rhein were significantly increased after oral administration of CT (P < 0.05), the half-life of ferulic acid in the CT group was lower than that in the TD group (P < 0.05) and the half-life and AUC 0-∞ of astragaloside IV in the CT group were significantly increased (P < 0.05), which revealed that wine-processing could influence the bioavailability and the elimination of these compounds. For better clinical efficacy, we suggest that the CT dosage form of QSP should be selected.

Exploring the Efficacy Enhancement Mechanism of Qixue Shuangbu prescription after TCM processing for treating chronic heart failure by regulating ERK/Bcl-2/Bax/Caspases-3 signaling pathway

“…Based on the TCM theory, different adjuvants might partake in physical or chemical transformation to alter pharmacological effects and exert the pharmacokinetic behavior for the enhanced therapeutic effect. In the previous study, we have detected differences in chemical compositions between CQSP and PQSP [ 26 ]. There were 101 common peaks of compounds in both CQSP and PQSP, in which 31 analytes increased and 38 analytes reduced in PQSP.…”

“…There were 101 common peaks of compounds in both CQSP and PQSP, in which 31 analytes increased and 38 analytes reduced in PQSP. The pharmacokinetic parameters of main bioactive components indicated longer lasting time elimination, better bioavailability and absorption for enhancing the effect in the treatment of CHF in the PQSP group than that of the CQSP group [ 17 , 26 ].…”

Synergistic mechanism of processing method for Qixue Shuangbu prescription in the treatment of chronic heart failure based on plasma metabolomics—Systematic bioinformatics

“…Qixue Shuangbu Prescription (QSP), a classical traditional Chinese medicine (TCM) prescription, has been widely applied in the treatment of chronic heart failure (CHF) [1,2]. Pi) at the ratio of 3:2:2:2:2:2:2:2:1 [3][4][5]. According to the requirements of TCM clinical medication, five Chinese medicinal herbs contained in QSP must be processed to improve the curative effect.…”

“…Druce ( Liliaceae , Yu Zhu), Polygonum multiflorum Thunb. ( Polygonaceae , He Shou Wu), Lycium barbarum L. ( Solanaceae , Gou Qi Zi), and Citrus reticulata Blanco ( Rutaceae , Chen Pi) at the ratio of 3:2:2:2:2:2:2:2:1 [3–5]. According to the requirements of TCM clinical medication, five Chinese medicinal herbs contained in QSP must be processed to improve the curative effect.…”

Comparative pharmacokinetics of 11 major bioactive components between two dosage forms of Qixue Shuangbu Prescription in rats by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

“…Previously, we have prepared QSP as a compound tincture, which can effectively improve bioavailability [19]. However, the alcohol in the tincture limits its range of application and reduces compliance.…”