Comparative performance analysis of Urised 3 and DIRUI FUS-200 automated urine sediment analyzers and manual microscopic method

Yalcinkaya, Emre; Erman, Hayriye; Kirac, Eray; Şerifoğlu, Afife; Aksoy, Alperen; İşman, Ferruh Kemal; Çekmen, Mustafa

doi:10.5222/mmj.2019.23169

Cited by 5 publications

(8 citation statements)

References 22 publications

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“…In addition, Bland-Altman agreement plot graphics demonstrated that automated microscopy units of the two devices showed acceptable performance for WBC (-29.3 ± 1.96 SD), RBC (43.3 ± 1.96 SD) and EPI (-4.0 ± 1.96 SD) cell counts [18] . Yalçınkaya et al [3] compared FUS200 and Urised3 urine analyzers where Deming regression analysis yielded a correlation coefficient of 0.961 and 0.961 for WBC and RBC counts, respectively. When they investigated the consistency between two urine analyzers for negative-positive test results, they found the kappa value to be 0.79 (good consistency) for WBC and 0.42 (moderate consistency) for RBC [3] .…”

Section: Discussionmentioning

confidence: 99%

“…Yalçınkaya et al [3] compared FUS200 and Urised3 urine analyzers where Deming regression analysis yielded a correlation coefficient of 0.961 and 0.961 for WBC and RBC counts, respectively. When they investigated the consistency between two urine analyzers for negative-positive test results, they found the kappa value to be 0.79 (good consistency) for WBC and 0.42 (moderate consistency) for RBC [3] . In the present study, Bland-Altman analysis revealed lower mean of differences between manual microscopy and UriSed2 device for WBC, RBC, EPI (-9.8, -13.5 and 7.7, respectively) suggesting that manual microscopy and UriSed2 device are concordant.…”

Section: Discussionmentioning

confidence: 99%

“…Microscopic examination of urine is affected by a number of factors including speed and duration of centrifugation, amount of urine left in the tube, dye usage, and experience and training of the analyst. Despite all these disadvantages, manual microscopy is the reference method for examination of urine sediment [3] . However, fully automated urine analyzers are recommended by many international institutions for minimizing these effects and for standardization of microscopic analysis [4] .…”

Section: Introductionmentioning

confidence: 99%

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LX-8000R and UriSed 2 fully automated urine analyzers comparison to manual microscopic examination

Erkan¹,

Aslan²

2022

J Med Biochemistry

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Background: Urinalysis has an important place in evaluating kidney and urinary tract infections. Automated urine analyzers enhance productivity and turnover in laboratories and economize time and labor required for analysis. In the present study, we evaluated and compared analytic and diagnostic performance of UriSed2 with LX-8000R, which is a novel image-based automated urine sediment analyzer. Methods: A total of 178 urine samples sent to our laboratory were evaluated by the two urine analyzers and standard manual microscopy. Precision and comparison studies were done in accordance with CLSI guidelines. Results: Sensitivity assessment revealed similar outcomes with both UriSed2 and LX-8000R devices for erythrocyte count (RBC), whereas UriSed2 device yielded higher outcomes for leukocyte count (WBC) and epithelial cells (EPI) than LX-8000R analyzer. Specificity of UriSed2 for WBC and RBC was higher than that of LX-8000R device. According to Gamma statistics, both urine analyzers showed perfect consistency for WBC, RBC and EPI cell counts. Manuel microscopy revealed statistically significant correlation between LX-8000R and UriSed2 in terms of WBC and RBC. Manual evaluation by Bland-Altman analysis demonstrated lower WBC and RBC values and higher EPI as compared to both UriSed2 and LX-8000R devices. As the result of Passing-Bablok regression analysis, both devices were found to be inconsistent with manual microscopy. Conclusion: We think that evaluation of automated urine analyzers will be more meaningful when they are evaluated together with urine samples and patient clinic in addition to comparing with manual microscopy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LX-8000R and UriSed 2 fully automated urine analyzers comparison to manual microscopic examination

Erkan¹,

Aslan²

2022

J Med Biochemistry

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“…Thus, automated urine autoanalyzers have been used in clinical laboratories with higher patient potential. To deal with the disadvantages of manual microscopy, automated urine analyzers using several analytical techniques have been developed [7]. Currently, these devices are frequently used in medical laboratories to minimize the work force lost during manual microscopic examination.…”

Section: Discussionmentioning

confidence: 99%

Retrospective Evaluation of the Performance of Leukocyte Parameter of the Automated Urine Analyzer and Its Concordance with Urine Culture

Koşer¹,

Işıksaçan²,

Korkusuz³

et al. 2021

GJU

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Objective: Complete urinalysis (CUA) is one of the indispensable screening tests of clinical laboratories. The compatibility of this test with urine culture is of indispensable importance in the diagnosis and treatment of urinary tract infections. We aimed to evaluate the suitability of the leukocyte parameter measured in the microscopic units of the fully automated urine analyzers which replace traditional methods, by grouping them according to the results of chemical analysis. Materials and Methods: Leukocyte counts in the reported CUA results of 4685 outpatients and the results of 113 urine cultures studied on the same day were analyzed. Noncentrifugated urine samples were included in the analysis. Cells were digitally imaged by flow microscopy. Chemical analyzes were performed using dual wavelength reflectance method. Urine samples were evaluated after 24 hours of incubation. Results: High power field (HPF) values were recorded by grouping the leukocyte counts as negative, trace, 1+, 2+ and 3+. The arithmetic means of HPF values of the groups were calculated as 1.2, 2.1, 5.0, 11 and 208 white blood cell (WBCs/HPF). Bacterial growth was detected in 19 of 113 patients and no reproduction was observed in the remaining 94 cases. When results of microscopic examinations and chemical analysis were compared with the culture results, the analytical sensitivity, specificity, positive, and negative predictive values for microscopic urinalysis were 25%, 86%, 61.3%, and 58.3%, respectively. While, the analytical sensitivity, specificity, positive, and negative predictive values for chemical analysis of urine were 25.8%, 87.7%, 69.7%, and 51.4%, respectively. Conclusion: The workload of medical laboratories is increasing, and the use of urine autoanalyzers may be preferred for busy laboratories. In the diagnosis and follow-up of urinary tract infections, complete urinalysis by autoanalysers in which the harmony of their microscopy and chemical units are closely monitored, may reduce the need for unnecessary requests for urine culture, but it cannot replace urine culture.

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“…In a study performed by Yalçınkaya et al with 440 urine samples based on the comparison of FUS200 and Urised3 microscopic urine analyzers with the Deming regres- sion analyzer, the correlation coefficients for WBC and RBC measurements performed by the two devices were 0.961 and 0.961, respectively. When they examined the concordance between the two microscopic analyzers for negative-positive test results, they found the Kappa value to be 0.79 (good agreement) for WBC and 0.42 (moderate agreement) for RBC [14]. In a study performed by İnigo et al with 1934 urine samples to compare the results obtained by SediMax and Sysmex UF1000i analyzers, they performed the Spearman correlation analysis between the devices and found a very strong correlation for WBC (r=0.928) and a strong correlation for RBC (r=0.631) and Squamous epithelium cell counts (r=0.631) between the devices [15].…”

Section: Discussionmentioning

confidence: 99%

A Comparative study on the performances of 77 Elektronika Urised 2-LabUmat 2 and Dirui FUS 200-H 800 urine analyzers

Erçin¹

2020

Int J Med Biochem

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A comparative study on the performances of 77 elektronika urised 2-LabUmat2 and Dirui FUS200 -H800 urine analyzersObjectives: This study aims to compare the analytical performance of the 77Elektronika brand LabUmat2-Urised2 model integrated urine analyzer and Dirui brand FUS200-H800 model integrated urine analyzer. Methods: Urine samples of 139 patients randomly selected from male and female patients of all age groups who were admitted to Ufuk University Faculty of Medicine, Dr. Ridvan Ege Hospital between 24.02.2020-28.02.2020 were analyzed on both devices. Results: The results of the samples whose WBC (white blood cell), RBC (red blood cell) and Squamous epithelial were measured using two different methods in microscopic analyzers were perfectly compatible in the same range and with three parameters (Gamma values 0.916; 0.770; 0.961, respectively). For the samples where crystal and cylinder tests were carried out in microscopic analyzers, Cappa values were 0.486 and 0.495, respectively. As the result of Passing-Bablok Regression analysis used for the method comparison of microscopic analyzers, Cusum test results were p<0.01, p=0.01 and p=0.65, respectively for the linearity in the measurement of WBC, RBC and Squamous epithelial. According to the Bland-Altman Compatibility Chart prepared to determine the difference between methods, the difference between the results of the measurements of WBC, RBC and Squamous epithelial in both devices were -29.3±1.

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Comparative performance analysis of Urised 3 and DIRUI FUS-200 automated urine sediment analyzers and manual microscopic method

Cited by 5 publications

References 22 publications

LX-8000R and UriSed 2 fully automated urine analyzers comparison to manual microscopic examination

LX-8000R and UriSed 2 fully automated urine analyzers comparison to manual microscopic examination

Retrospective Evaluation of the Performance of Leukocyte Parameter of the Automated Urine Analyzer and Its Concordance with Urine Culture

A Comparative study on the performances of 77 Elektronika Urised 2-LabUmat 2 and Dirui FUS 200-H 800 urine analyzers

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