Comparative morphological, anatomical and palynological studies on Trigonella (Fabaceae) in Khorassan Razavi province (NE Iran)

Abstract

Morphological, anatomical and palynological characters of five species of Trigonella growing in Khorassan Razavi province were examined. In morphological study, the differences of vegetative and floral features were assessed. Then cluster analysis was done using UPGMA to recognize the affinity between species. Moreover PCA analysis was carried out to identify species diversity and valuable features for species identification. For anatomical research, the cross sections of the stems and leaflets were prepared and stained by differential staining. In palynological study, the pollen were extracted, acetolysed and observed by SEM. The results of cluster analysis showed circumscription between species and sections. Moreover PCA analysis indicated species diversity and useful traits for identification. The anatomical results displayed some variations in vessel arrangement and cell type of xylary fiber in stem between species. Furthermore, pollen ornamentation was variable among species and helped to identify them.

Palyno‐anatomical microscopic characterization of selected species of Boraginaceae and Fabaceae

“…M. monantha pollens were analyzed by Pinar et al (2014), as prolate to spheroidal, tricolporate, perforate similar to that of our study except oblate to spheroidal shape. Ghadiri, Jafari, and Hamdi (2014), reported pollen shape as prolate‐sub‐prolate which are also showing variation in pollen shape of our findings. According to Perveen and Qaiser (1998), pollens of S. italica were prolate‐spheroidal, regulate, faveolate, and reticulate.…”