Genetic Population Structure of Dastarcus helophoroides (Coleoptera: Bothrideridae) From Different Long-Horned Beetle Hosts Based on Complete Sequences of Mitochondrial COI

Abstract

Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of long-horned beetles in China, Japan, and Korea. In this study, the genetic sequence of cytochrome oxidase subunit Ι was used to investigate the genetics and relationships within and among D. helophoroides populations collected from five different geographic locations. We used principal component analysis, heatmap, and Venn diagram results to determine the relationship between haplotypes and populations. In total, 26 haplotypes with 51 nucleotide polymorphic sites were defined, and low genetic diversity was found among the different populations. Significant genetic variations were observed mainly within populations, and no correlation was found between genetic distribution and geographical distance. Low pairwise fixation index values (-0.01424 to 0.04896) and high gene flows show that there was high gene exchange between populations. The codistributed haplotype DH01 was suggested to be the most ancestral haplotype, and other haplotypes were thought to have evolved from it through several mutations. In four of the populations, both common haplotypes (DH01, DH03, and DH22) and unique haplotypes were found. Low genetic diversity among different populations is related to a relatively high flight capacity, host movement, and human-aided dispersal of D. helophoroides. The high gene exchange and typically weak population genetic structure among five populations, especially among populations of Anoplophora glabripennis (Motschulsky), Monochamus alternatus (Hope), and Massicus raddei (Blessig), may suggest that these populations cross naturally in the field.

Chromosome‐level genome assembly of Dastarcus helophoroides provides insights into CYP450 genes expression upon insecticide exposure

“…Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important ectoparasitoid of Cerambycidae beetles that is widely used in their biological control 7–9 . Indoor mass‐reared Dastarcus helophoroides has been widely released into woodland and achieved significant results of controlling Anoplophora glabripennis (Motschulsky), Monochamus alternatus , Massicus raddei (Blessig), Batocera horsfieldi (Hope), and Apriona swainsoni (Hope) around China 10,11 . By releasing the parasitoid, the highest and average parasitism rate of Dastarcus helophoroides on Monochamus alternatus were 92.6% and 88.6% in the first year, respectively, and the spread of pine wilt disease vectored by Monochamus alternatus had been successfully controlled 11 .…”

“…[7][8][9] Indoor mass-reared Dastarcus helophoroides has been widely released into woodland and achieved significant results of controlling Anoplophora glabripennis (Motschulsky), Monochamus alternatus, Massicus raddei (Blessig), Batocera horsfieldi (Hope), and Apriona swainsoni (Hope) around China. 10,11 By releasing the parasitoid, the highest and average parasitism rate of Dastarcus helophoroides on Monochamus alternatus were 92.6% and 88.6% in the first year, respectively, and the spread of pine wilt disease vectored by Monochamus alternatus had been successfully controlled. 11 The adult Dastarcus helophoroides is sensitive to lambda-cyhalothrin in laboratory condition, with low concentrations leading to an adult showing the dorsal surface down and ventral surface up.…”

“…10,11 By releasing the parasitoid, the highest and average parasitism rate of Dastarcus helophoroides on Monochamus alternatus were 92.6% and 88.6% in the first year, respectively, and the spread of pine wilt disease vectored by Monochamus alternatus had been successfully controlled. 11 The adult Dastarcus helophoroides is sensitive to lambda-cyhalothrin in laboratory condition, with low concentrations leading to an adult showing the dorsal surface down and ventral surface up. Adults lose the ability of crawling and feeding in this knockdown, which has a significant impact on its life activities.…”

Genetic structure and population demographics of Hypnoidus bicolor (Coleoptera: Elateridae) in the Canadian Prairies

“…Although it is widely used in species diagnostics, its robustness and comparatively strong phylogenetic signal make this gene ideal for characterizing genetic variation in natural populations and reconstructing gene genealogies 18,19 . Consequently, COX1 has been amplified to assess genetic structure and/or delineate pest species of coleopterans sampled from a wide variety of habitats and geographical regions 20–25 . In our studies, we sequenced a 622‐bp region of the COX1 gene from H. bicolor individuals collected across the Canadian Prairies in order to: (i) determine population genetic structure and demographics of H. bicolor , and (ii) unravel cryptic forms associated with H. bicolor “species complex.”…”