The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied.
To compare clinicophysiological, haematobiochemical and haemodynamic effects of propofol and ketamine total intravenous anaesthesia (TIVA) with dexmedetomidine in uraemic goats.
Supratentorial atypical teratoid rhabdoid tumors (AT/RTs) of infancy and childhood are rare, highly malignant neoplasms, most common in the first 2 years of life. In spite of multiple treatment regimens consisting of surgical resection, radiation therapy, and multi-agent chemotherapy, the prognosis is very poor. The majority of these tumors are located in the cerebellum, cerebellopontine angle, pineal gland, spinal cord, and the suprasellar region; supratentorial location is relatively uncommon, and the intraventricular location is extremely rare. We report a rare case of AT/RT arising in the lateral ventricle in a 4-year-old patient.
Background:
With the outbreak of high throughput next-generation sequencing (NGS), the biological
research of drug discovery has been directed towards the oncology and infectious disease therapeutic areas, with
extensive use in biopharmaceutical development and vaccine production.
Method:
In this review, an effort was made to address the basic background of NGS technologies, potential applications
of NGS in drug designing. Our purpose is also to provide a brief introduction of various Nextgeneration
sequencing techniques.
Discussions:
The high-throughput methods execute Large-scale Unbiased Sequencing (LUS) which comprises of
Massively Parallel Sequencing (MPS) or NGS technologies. The Next geneinvolved necessarily executes Largescale
Unbiased Sequencing (LUS) which comprises of MPS or NGS technologies. These are related terms that
describe a DNA sequencing technology which has revolutionized genomic research. Using NGS, an entire human
genome can be sequenced within a single day.
Conclusion:
Analysis of NGS data unravels important clues in the quest for the treatment of various lifethreatening
diseases and other related scientific problems related to human welfare.
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