Comparative metabolome analysis of wheat embryo and endosperm reveals the dynamic changes of metabolites during seed germination

Han, Caixia; Zhen, Shoumin; Zhu, Guijie; Bian, Yanwei; Yan, Yueming

doi:10.1016/j.plaphy.2017.04.013

Cited by 62 publications

(53 citation statements)

References 36 publications

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“…It was estimated that for each centimeter that the sowing depth increases, there is a reduction of 4.8% of emergence speed index and an increase of 6.4% in the mean emergence time of wheat ( Figure 1A and 1B). This is due to the higher demand for seed energy reserves for the germination and emergence stages (Han et al, 2017), which also delay the development of the aerial part, responsible for producing photo-assimilates that will be translocated in the plant.…”

Section: Resultsmentioning

confidence: 99%

“…These results can be verified by metabolomic analysis in which the mobilization of the present reserves both in the embryo and the endosperm of the wheat seeds, was observed during the first hours after the beginning of the germination process. It culminates in the breakdown of carbohydrates, proteins, lipids, among other sources, into sugars used in the establishment of the seedling (Han et al, 2017). In this way, the high initial availability of energy facilitates the rapid development of aerial and root structures of the seedlings until the beginning of photosynthetic processes and the absorption of nutrients.…”

Section: A Bmentioning

confidence: 99%

See 1 more Smart Citation

Does Azospirillum brasilense and Stimulate® to improve the initial growth of wheat sown at greater depths?

Zóz

Vendruscolo

Witt³

et al. 2019

Agraria

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The sowing of the wheat crop is a delicate process given the reduced size of its seed. Errors in adjusting the depth of sowing of this crop may result in damage to the establishment and initial growth of the crop. This study aimed to evaluate the influence of the relationship between inoculation with Azospirillum brasilense, Stimulate ® treatment and sowing depth on emergence and initial growth of wheat plants. The experimental design was a randomized complete block design with four replications, in a 4 x 4 factorial scheme. The first factor consisted of the following treatments: i) seed inoculation with A. brasilense, ii) seed treatment with Stimulate ® , iii) inoculation of seeds with A. brasilense associated with the treatment of seeds with Stimulate ® and iv) without inoculation and seed treatment. The second factor was constituted by four sowing depths, being: 1.0; 3.0; 5.0 and 7.0 cm. The treatment of seeds with Stimulate ® favors the higher accumulation of dry mass of stem in wheat plants when sown in greater depths. Seeding at greater depths up to 7.0 cm resulted in the delayed emergence of seedlings, a smaller number of tillers and less dry matter accumulation in wheat plants. Azospirillum brasilense e Stimulate ® favorecem o crescimento inicial do trigo semeado em maiores profundidades?RESUMO: A semeadura da cultura do trigo é um processo delicado tendo em vista o tamanho reduzido de sua semente. Erros no ajuste da profundidade de semeadura desta cultura podem resultar em prejuízos para o estabelecimento e crescimento inicial da cultura. Este estudo teve por objetivo avaliar a influência da relação entre a inoculação com Azospirillum brasilense, o tratamento com Stimulate ® e a profundidade de semeadura sobre a emergência e o crescimento inicial de plantas de trigo. O delineamento experimental utilizado foi o de blocos ao acaso com quatro repetições, em esquema fatorial 4 x 4. O primeiro fator foi constituído pelos seguintes tratamentos: i) inoculação de sementes com A. brasilense, ii) tratamento de sementes com Stimulate ® , iii) inoculação de sementes com A. brasilense associado ao tratamento de sementes com Stimulate ® e iv) sem inoculação e tratamento de sementes. O segundo fator foi constituído por quatro profundidades de semeadura, sendo: 1,0; 3,0; 5,0 e 7,0 cm. O tratamento de sementes com Stimulate ® favorece o maior acúmulo de massa seca de colmo em plantas de trigo quando semeado em maiores profundidades. A semeadura em maiores profundidades, até 7,0 cm, tem como consequência o atraso na emergência plântulas e, a redução do número de afilhos e acúmulo de massa seca em plantas de trigo.Palavras-chave: fixação biológica; bactéria diazotrófica; emergência; afilho; Triticum aestivum L.Does Azospirillum brasilense and Stimulate ® to improve the initial growth of wheat sown at greater depths?Rev. Bras.

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Section: Resultsmentioning

confidence: 99%

Section: A Bmentioning

confidence: 99%

Does Azospirillum brasilense and Stimulate® to improve the initial growth of wheat sown at greater depths?

Zóz

Vendruscolo

Witt³

et al. 2019

Agraria

View full text Add to dashboard Cite

show abstract

“…Han et al (2017) used PCA, to reveal distinct dynamic changes in metabolites between the embryo and endosperm during wheat germination. The total variance explained by the two principal components was 68.9%, being 38.3% and 30.6% for component 1 (PC1) and 2 (PC2), respectively ( Figure 5).…”

Section: Resultsmentioning

confidence: 99%

Biochemical profiling and its role in physiological quality of maize seeds

2018

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-Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.Index terms: vigor, viability, genetic divergence, stored reserves, Zea mays.Perfil bioquímico e seu papel na qualidade fisiológica de sementes de milho RESUMO -A viabilidade e o vigor de sementes são atributos fisiológicos influenciados por uma série de fatores, incluindo o genótipo. O perfil bioquímico das sementes de milho e sua correlação com a qualidade fisiológica são importantes para programas de melhoamento que objetivam obter sementes de melhor qualidade. O objetivo deste estudo foi analisar a diversidade genética entre as linhagens de milho e avaliar os componentes bioquímicos que têm um papel importante na qualidade fisiológica das sementes. As sementes das linhagens e dos híbridos foram submetidas ao teste de viabilidade, vigor pelo envelhecimento acelerado e tolerância ao frio e a determinação do perfil bioquímico (teor de proteína total, proteínas solúveis, fósforo total, fitato, fósforo inorgânico, amido e açúcares solúveis). A divergência genética encontrada entre as linhagens permite concluir que os ganhos genéticos podem existir em associação com a qualidade fisiológica dos híbridos de milho. As sementes com maior teor de fósforo inorgânico e açúcares solúveis apresentaram qualidade fisiológica superior à das sementes com níveis menores desses compostos.Termos para indexação: vigor, viabilidade, divergência genética, reservas armazenadas, Zea mays.

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“…Phase II is named as the absorbing platform. It is the critical phase of germination because gene expression and metabolic activity are largely enhanced [20,21]. Phase III is indicated with the appearance of the hypocotyl and continued water absorption and seedling growth.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress

Shen

Zhang

Liu

et al. 2020

IJMS

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Gossypium hirsutum L., is a widely cultivated cotton species around the world, but its production is seriously threatened by its susceptibility to chilling stress. Low temperature affects its germination, and the underlying molecular mechanisms are rarely known, particularly from a transcriptional perspective. In this study, transcriptomic profiles were analyzed and compared between two cotton varieties, the cold-tolerant variety KN27-3 and susceptible variety XLZ38. A total of 7535 differentially expressed genes (DEGs) were identified. Among them, the transcripts involved in energy metabolism were significantly enriched during germination based on analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as glycolysis/gluconeogenesis, tricarboxylic acid cycle (TCA cycle), and glyoxylate cycle (GAC). Results from further GO enrichment analysis show the earlier appearance of DNA integration, meristem growth, cotyledon morphogenesis, and other biological processes in KN27-3 compared with XLZ38 under chilling conditions. The synthesis of asparagine, GDP-mannose, and trehalose and the catabolic process of raffinose were activated. DEGs encoding antioxidants (spermidine) and antioxidase (CAT1, GPX4, DHAR2, and APX1) were much more up-regulated in embryos of KN27-3. The content of auxin (IAA), cis-zeatin riboside (cZR), and trans-zeatin riboside (tZR) in KN27-3 are higher than that in XLZ38 at five stages (from 12 h to 54 h). GA3 was expressed at a higher level in KN27-3 from 18 h to 54 h post imbibition compared to that in XLZ38. And abscisic acid (ABA) content of KN27-3 is lower than that in XLZ38 at five stages. Results from hormone content measurements and the related gene expression analysis indicated that IAA, CTK, and GA3 may promote germination of the cold-tolerant variety, while ABA inhibits it. These results expand the understanding of cottonseed germination and physiological regulations under chilling conditions by multiple pathways.

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Comparative metabolome analysis of wheat embryo and endosperm reveals the dynamic changes of metabolites during seed germination

Cited by 62 publications

References 36 publications

Does Azospirillum brasilense and Stimulate® to improve the initial growth of wheat sown at greater depths?

Does Azospirillum brasilense and Stimulate® to improve the initial growth of wheat sown at greater depths?

Biochemical profiling and its role in physiological quality of maize seeds

Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress

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