Comparative genome characterization of Achromobacter members reveals potential genetic determinants facilitating the adaptation to a pathogenic lifestyle

Li, Xiangyang; Hu, Yue; Gong, Jing; Zhang, Lin-Shuang; Wang, Gejiao

doi:10.1007/s00253-013-5018-3

Cited by 50 publications

(56 citation statements)

References 65 publications

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“…Moreover, rapid intraclonal microevolution has been described and related to habitat adaptation (25). Similar adaptive behavior has been suggested by genomics in the genus Achromobacter (26).…”

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confidence: 55%

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Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Abdouchakour

Dupont

Grau

et al. 2015

Appl Environ Microbiol

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Dental care unit waterlines (DCUWs) consist of complex networks of thin tubes that facilitate the formation of microbial biofilms. Due to the predilection toward a wet environment, strong adhesion, biofilm formation, and resistance to biocides, Pseudomonas aeruginosa, a major human opportunistic pathogen, is adapted to DCUW colonization. Other nonfermentative Gramnegative bacilli, such as members of the genus Achromobacter, are emerging pathogens found in water networks. We reported the 6.5-year dynamics of bacterial contamination of waterlines in a dental health care center with 61 dental care units (DCUs) connected to the same water supply system. The conditions allowed the selection and the emergence of clones of Achromobacter sp. and P. aeruginosa characterized by multilocus sequence typing, multiplex repetitive elements-based PCR, and restriction fragment length polymorphism in pulsed-field gel electrophoresis, biofilm formation, and antimicrobial susceptibility. One clone of P. aeruginosa and 2 clones of Achromobacter sp. colonized successively all of the DCUWs: the last colonization by P. aeruginosa ST309 led to the closing of the dental care center. Successive dominance of species and clones was linked to biocide treatments. Achromobacter strains were weak biofilm producers compared to P. aeruginosa ST309, but the coculture of P. aeruginosa and Achromobacter enhanced P. aeruginosa ST309 biofilm formation. Intraclonal genomic microevolution was observed in the isolates of P. aeruginosa ST309 collected chronologically and in Achromobacter sp. clone A. The contamination control was achieved by a complete reorganization of the dental health care center by removing the connecting tubes between DCUs. D ental instruments on dental care units (DCUs), such as ultrasonic scalers, air scalers, and high-speed turbine dental handpieces, are cooled by DCU-supplied water, which also irrigates and cools the tooth surface during dental treatment (1). Water is also supplied to the DCU cup filler outlet used by patients for oral rinsing and to the bowl rinse outlet rinsing the DCU spittoon. Therefore, DCU waterlines (DCUWs) consist of a complex network formed by meters of narrow-bore plastic tubing of mostly 2-to 3-mm internal diameter. In narrow-bore tubes, a thin immobile layer of fluid, called the hydrodynamic boundary layer, exists at the interface of the lumen wall and the moving water (2). This hydrodynamic phenomenon is associated with water stagnation when the DCU is not used, and heating of water to at least 20°C enhances the development of microbial biofilm in the tubes (3).The DCUWs appear to be a technological niche in which complex interactions occur among members of the microbial community (4-6). Regarding bacteria, DCUWs have been shown to promote the formation of biofilm, predominantly seeded from environmental bacteria in the water supply (4-6). More rarely, human bacteria such as staphylococci or oral bacteria have been detected, indicating a retraction of oral fluids from the surgical devices to the wate...

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confidence: 55%

“…6 and 7). According the criteria of Stepanovic (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34), P. aeruginosa strains appeared to be high biofilm producers, whereas Achromobacter sp. strains were weak producers or nonproducers under all conditions tested.…”

Section: Synopsis Of DCC Bacterial Contaminationmentioning

confidence: 99%

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Abdouchakour

Dupont

Grau

et al. 2015

Appl Environ Microbiol

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“…In bacteria, five different efflux pump families have been identified (37): major facilitator superfamily (MFS), multidrug and toxic compound extrusion (MATE), small multidrug resistance (SMR), ATP-binding cassette (ABC), and resis- tance-nodulation-division (RND). Comparative genome characterization of six Achromobacter members showed that, compared with other genera, Achromobacter strains contain a high number of efflux pump-associated genes, ranging from 40 to 53 genes in different isolates (38). Similarly, in the A. xylosoxidans NH44784-1996 complete genome, 45 pump-related genes were predicted (39).…”

Section: Discussionmentioning

confidence: 99%

Genomic Insights into Intrinsic and Acquired Drug Resistance Mechanisms in Achromobacter xylosoxidans

Zhu

et al. 2015

Antimicrob Agents Chemother

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e Achromobacter xylosoxidans is an opportunistic pathogen known to be resistant to a wide range of antibiotics; however, the knowledge about the drug resistance mechanisms is limited. We used a high-throughput sequencing approach to sequence the genomes of the A. xylosoxidans type strain ATCC 27061 and a clinical isolate, A. xylosoxidans X02736, and then we used different bioinformatics tools to analyze the drug resistance genes in these bacteria. We obtained the complete genome sequence for A. xylosoxidans ATCC 27061 and the draft sequence for X02736. We predicted a total of 50 drug resistance-associated genes in the type strain, including 5 genes for ␤-lactamases and 17 genes for efflux pump systems; these genes are also conserved among other A. xylosoxidans genomes. In the clinical isolate, except for the conserved resistance genes, we also identified several acquired resistance genes carried by a new transposon embedded in a novel integrative and conjugative element. Our study provides new insights into the intrinsic and acquired drug resistance mechanisms in A. xylosoxidans, which will be helpful for better understanding the physiology of A. xylosoxidans and the evolution of antibiotic resistance in this bacterium.

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“…The organism was first isolated and described by Yabuuchi and Oyama in 1971, reclassified to the Alcaligenes genus, and more recently placed back in the Achromobacter genus (4,5). Recent genomic sequencing has shown that the genus is most closely related to Bordetella than to Alcaligenes, with one study suggesting a recent common ancestor between Bordetella and Achromobacter, and proposing a shared "supergenus" status between members of both genera (6). Further complicating the phylogenic picture, there are numerous genetically distinct Achromobacter species and subspecies that have yet to be fully characterized (6,7).…”

mentioning

confidence: 99%

Achromobacter Respiratory Infections

Swenson

Sadikot²

2015

Annals ATS

102

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Achromobacteria are ubiquitous environmental organisms that may also become opportunistic pathogens in certain conditions, such as cystic fibrosis, hematologic and solid organ malignancies, renal failure, and certain immune deficiencies. Some members of this genus, such as xylosoxidans, cause primarily nosocomially acquired infections affecting multiple organ systems, including the respiratory tract, urinary tract, and, less commonly, the cardiovascular and central nervous systems. Despite an increasing number of published case reports and literature reviews suggesting a global increase in achromobacterial disease, most clinicians remain uncertain of the organism's significance when clinically isolated. Moreover, effective treatment can be challenging due to the organism's inherent and acquired multidrug resistance patterns. We reviewed all published cases to date of non-cystic fibrosis achromobacterial lung infections to better understand the organism's pathogenic potential and drug susceptibilities. We found that the majority of these cases were community acquired, typically presenting as pneumonias (88%), and were most frequent in individuals with hematologic and solid organ malignancies. Our findings also suggest that achromobacterial lung infections are difficult to treat, but respond well to extended-spectrum penicillins and cephalosporins, such as ticarcillin, piperacillin, and cefoperazone.

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Comparative genome characterization of Achromobacter members reveals potential genetic determinants facilitating the adaptation to a pathogenic lifestyle

Cited by 50 publications

References 65 publications

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Genomic Insights into Intrinsic and Acquired Drug Resistance Mechanisms in Achromobacter xylosoxidans

Achromobacter Respiratory Infections

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