Comparative evaluation of the three different surface treatments - conventional, laser and Nano technology methods in enhancing the surface characteristics of commercially pure titanium discs and their effects on cell adhesion: An in vitro study

Vignesh,; Nayar, Sanjna; Bhuminathan,; Mahadevan,; Santhosh, S.

doi:10.4103/0975-7406.155817

Cited by 14 publications

(14 citation statements)

References 12 publications

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“…Depending on the study the way of abundant decontamination was slightly different. Titanium was treated with different lasers i.e., Er:YAG [ 49 ], erbium fiber laser [ 52 ], ytterbium fiber laser [ 53 ], Ti:Sapphire laser fs-system laser [ 44 , 46 ], Nd:YAG [ 47 ], Nd:YVO 4 [ 51 ] , CO 2 laser [ 48 ], KrF excimer laser [ 45 ], diode laser [ 50 ]. In eleven studies laser was the only treatment applied.…”

Section: Resultsmentioning

confidence: 99%

“…The full-text articles review found heterogeneity in the papers. All the studies included in the review concerned fibroblasts—whether human cells from biopsies [ 46 , 49 , 50 , 51 ] or cell culture [ 52 , 53 ] or mouse embryonic cells [ 44 , 47 , 48 ]. In one study, cell origin was not defined [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…Thanks to this modification the fibroblasts were prompted to grow and to form pseudopods which could influence better adhesion. The lasers used in included papers could be divided into the following groups: Nd:YAG [ 47 ], Nd:YVO 4 [ 51 ], Er:YAG [ 49 ], erbium fiber laser [ 52 ], ytterbium fiber laser [ 52 ], Ti:Sapphire laser [ 44 , 46 ], diode laser [ 49 ], KrF excimer laser [ 45 ] and CO 2 laser [ 48 ] ( Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…The articles included in the review were qualified as high-quality scoring 7/9 [ 44 , 46 , 48 , 49 , 53 ], 8/9 [ 47 , 52 ] or 9/9 points [ 50 ]. None of the studies was obtained and excluded as low-quality or moderate paper, but some of the studies were qualified as a moderate risk of bias scoring 6/9 points [ 45 , 51 ] ( Table 5 ).…”

Section: Resultsmentioning

confidence: 99%

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Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review

et al. 2021

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Objective: Laser treatment has been recently introduced in many fields of implant dentistry. The systematic review tried to address the question: “How does laser modification of titanium surface influence fibroblast adhesion?”. Methods: An electronic search of the PubMed and Scopus databases was performed. The following keywords were used: (laser) AND (fibroblast) AND (titanium) AND (implant OR disc) AND (proliferation OR adhesion). Initially, 136 studies were found. Ten studies met the inclusion criteria and were included in the review. All studies chosen to be included in the review were considered to have a low risk of bias. Results: Studies included in the review varied with laser parameters or ways of observing fibroblast behavior. Studies showed that fibroblasts tend to take different shapes and create extensions on modified surfaces and that their metabolic activity is more intense. One study concentrated on laser application and showed that three-directional laser application is the most successful in terms of fibroblast adhesion. Studies which concentrated more on laser parameters showed that too low energy density (lower or equal to 0.75 J/cm2) does not influence fibroblast adhesion. Increasing the energy density over 0.75 J/cm2 causes better cell adhesion of fibroblasts to the laser-modified sample. One included study focused on increasing titanium surface wettability, which also positively influenced cell adhesion. Conclusion: The studies included in the review proved a positive effect of laser-modified titanium surfaces on fibroblast adhesion. However, the application of an appropriate laser energy dose is crucial.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review

et al. 2021

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“…It is speculated that the surface of the 2D scaffolds provided anchorage points for initial attachment to hold the cell body in place, allowing subsequent filopodial protrusion to regulate cell shape and further growth [40]. Indeed, more numerous filopodial structures on 2D PCL scaffolds ( Figure 9) have been shown to promote greater cell attachment [41,42]. This result was further verified by the increase of FAK expression described here (Figure 7), as filopodial shafts contain signaling pathways (e.g., FAK, Rac1/Cdc42/RhoA) promoting substrate adhesion [43].…”

Section: Discussionmentioning

confidence: 99%

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Inthanon

Janvikul

Ongchai

et al. 2019

JFB

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Polycaprolactone (PCL), a hydrophobic-degradable polyester, has been widely investigated and extensively developed, to increase the biocompatibility for tissue engineering. This research was the first trial to evaluate the intrinsic biological responses of human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) cultured on alkaline hydrolysis and low-pressure oxygen plasma modified 2D and 3D PCL scaffolds, without adding any differentiation inducers; this has not been reported before. Four types of the substrate were newly established: 2D plasma-treated PCL (2D-TP), 2D non-plasma-treated PCL (2D-NP), 3D plasma-treated PCL (3D-TP), and 3D non-plasma-treated PCL (3D-NP). Physicochemical characterization revealed that only plasma-treated PCL scaffolds significantly increased the hydrophilicity and % oxygen/carbon ratio on the surfaces. The RMS roughness of 3D was higher than 2D conformation, whilst the plasma-treated surfaces were rougher than the non-plasma treated ones. The cytocompatibility test demonstrated that the 2D PCLs enhanced the initial cell attachment in comparison to the 3Ds, indicated by a higher expression of focal adhesion kinase. Meanwhile, the 3Ds promoted cell proliferation and migration as evidence of higher cyclin-A expression and filopodial protrusion, respectively. The 3Ds potentially protected the cell from apoptosis/necrosis but also altered the pluripotency/differentiation-related gene expression. In summary, the different configuration and surface properties of PCL scaffolds displayed the significant potential and effectiveness for facilitating stem cell growth and differentiation in vitro. The cell–substrate interactions on modified surface PCL may provide some information which could be further applied in substrate architecture for stem cell accommodation in cell delivery system for tissue repair.

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In vitro biological outcome of laser application for modification or processing of titanium dental implants

2017

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There are numerous functions for laser in modern implant dentistry including surface treatment, surface coating, and implant manufacturing. As laser application may potentially improve osseointegration of dental implants, we systematically reviewed the literature for in vitro biological responses to laser-modified or processed titanium dental implants. The literature was searched in PubMed, ISI Web, and Scopus, using keywords "titanium dental implants," "laser," "biocompatibility," and their synonyms. After screening the 136 references obtained, 28 articles met the inclusion criteria. We found that Nd:YAG laser was the most commonly used lasers in the treatment or processing of titanium dental implants. Most of the experiments used cell attachment and cell proliferation to investigate bioresponses of the implants. The most commonly used cells in these assays were osteoblast-like cells. Only one study was conducted in stem cells. These in vitro studies reported higher biocompatibility in laser-modified titanium implants. It seems that laser radiation plays a vital role in cell response to dental implants; however, it is necessary to accomplish more studies using different laser types and parameters on various cells to offer a more conclusive result.

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Comparative evaluation of the three different surface treatments - conventional, laser and Nano technology methods in enhancing the surface characteristics of commercially pure titanium discs and their effects on cell adhesion: An in vitro study

Cited by 14 publications

References 12 publications

Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review

Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

In vitro biological outcome of laser application for modification or processing of titanium dental implants

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