Comparative evaluation of four commercial tests for presumptive identification of Candida albicans

Cárdenes, C.D.; Carrillo-Muñoz, Alfonso Javier; Arias, Á.; Rodríguez-Álvarez, Cristobalina; Torres-Lana, A.; Sierra, A.; Arévalo, M.P.

doi:10.1016/j.mimet.2004.07.009

Cited by 19 publications

(13 citation statements)

References 15 publications

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“…An appropriate primary cu lture med iu m that assists in the recovery and differentiation of colonies which are phenotypically similar is a vital requirement for the laboratory detection of mixed fungal clinical specimens. Traditional methods for identification of yeast pathogens involves several days and specific mycology media wh ile chromogenic med ia contains chromogenic substrates which react with enzy mes secreted by the organisms to give colour reactions for d ifferent species [9] thus complementing traditional methods of identification [11]. CHROMagar™ Candida is the most well-known and widely used chromogenic mediu m for the identificat ion of different Candida species and is the most expensive of the five chromogenic media .…”

Section: Discussionmentioning

confidence: 99%

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Messeir¹,

Abrantes²,

Africa³

2012

MICROBIOLOGY

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The treat ment of invasive candidiasis and other Candida infections with the appropriate antifungal agent is assisted by the identification of Candida isolates to the species level. Rapid and accurate methods of differentiation are therefore imperat ive if t reat ment is to be effective, particularly in HIV-positive patients and in pregnant mothers where intervention may be necessary to reduce the risk for preterm delivery. The time used for isolation, identificat ion and detection of mixed cultures may be reduced with the help of available chro mogenic med ia. In this study, five commercial chro mogenic med ia were evaluated for the differentiation of Candida species. Six type-strains of Candida species were streaked onto each of five different chro mogenic media and incubated for up to 4 days at the different temperatures recommended by the manufacturers. This co mparative evaluation demonstrated the strengths and weaknesses of each mediu m employed and found CHROMagar™ Candida and Chro mogenic Candida Agar to be the most effective for distinguishing between different Candida species.

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Section: Discussionmentioning

confidence: 99%

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Messeir¹,

Abrantes²,

Africa³

2012

MICROBIOLOGY

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“…Comparing with traditional methods, our CNTFETs only need 1 h to detect C. albicans while conventional media requires 24-48 h for the interpretation of the results [38,4,39]. In addition, in our sensor devices the recognition element was a specific antibody anti-C.…”

Section: Resultsmentioning

confidence: 99%

“…This is an advantage comparing for instance with chromogenic culture media, which contain chromogenic or fluorogenic substrates for a rapid identification of C. albicans on the basis of colony colour. Although some of them present high sensitivity and specificity (97% and 92%, respectively), false positive results are frequently obtained [38,39].…”

Section: Resultsmentioning

confidence: 99%

Improved detection of Candida albicans with carbon nanotube field-effect transistors

Villamizar

Maroto

Rius

2009

Sensors and Actuators B: Chemical

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“…CHROMagar ® Candida medium (Becton Dickenson, Sparks, MD, USA), containing a mixture of chromogenic substrates, was used for presumptive identification and assessment of beta‐ N ‐acetylhexosaminidase (HexNAcase) production 12,18 . A single yeast colony grown on Sabouraud agar plate (incubated at 30 °C for 48 h) was streaked (replicated) onto the CHROMagar ® Candida medium and incubated for 48 h at 37 °C under aerobic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Candida albicans was reported 10,11 to exist under different pleomorphic forms. Each of these forms (blastoconidia, germ tube, pseudohyphae and true hyphae) provides critical functions required for the pathogenic lifestyle 12,13 . A unicellular yeast form, by changing its morphotype into the hyphal form, is able to penetrate various physiological barriers 5,14–16 .…”

Section: Introductionmentioning

confidence: 99%

Discrimination between the enzymatic activities of Candida albicans pleomorphic forms determined using the api® ZYM test

Staniszewska

Rabczenko

Kurzątkowski

2011

Mycoses

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Enzymatic activity profiles for two morphotypes of 37 Candida albicans clinical isolates were compared. Yeast and hyphal forms were grown using yeast extract-peptone-glucose broth or undiluted human serum, respectively. Both morphotypes were documented under scanning electron microscopy. The api(®) ZYM (BioMérieux, France) test was used to evaluate the enzymatic activity profiles for particular pleomorphic forms. None of the examined enzymatic activities showed good agreement (kappa, κ > 0.80) for the two morphotypes of the tested strains. Only leucine arylamidase activity in blastoconidia and hyphae of 35 out of 37 strains appeared to be in significant agreement (κ = 0.770). This phenomenon should be explored further for clinical benefits. For morphotypes of all tested strains, activity profiles of 11 hydrolytic enzymes demonstrated weak agreement (κ = 0.044-0.197). Moreover, satisfactory (κ = 0.218-0.348) and moderate agreement (κ = 0.413-0.479) were noted for enzymatic activity values of five and two enzymes, respectively. The distinct differences in activity profiles of hydrolytic enzymes between hyphae and blastoconidia is suggested to be related to the specific roles of these two morphotypes in particular steps of pathogenesis. Moreover, both morphotypes should be examined by strain biotyping methods. Beta-N-hexosaminidase (HexNAcase) activity assessed by the api(®) ZYM test and on CHROMagar Candida(®) medium (Becton Dickinson, USA) is also discussed.

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Comparative evaluation of four commercial tests for presumptive identification of Candida albicans

Cited by 19 publications

References 15 publications

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Improved detection of Candida albicans with carbon nanotube field-effect transistors

Discrimination between the enzymatic activities of Candida albicans pleomorphic forms determined using the api® ZYM test

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