Comparative evaluation of cysticercal antigens and immunoassays in the diagnosis of neurocysticercosis

Katti, Muralidhar K.; Chandramukhi, A

doi:10.1080/00034983.1991.11812616

Cited by 18 publications

(17 citation statements)

References 10 publications

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“…A wide disparity in the specificity of the highly sensitive immunoassays has been attributed to the use of the crude antigens (Espinoza et al 1986;Estrada et al 1989;Tsang et al 1989;Katti and Chandramukhi 1991;Malla et al 1992;Mandal et al 2006). Del Brutto et al (2001) laid down certain guidelines to ease out the diagnostic dilemmas regarding this disease and included a positive serum EITB as a major criterion.…”

Section: Discussionmentioning

confidence: 99%

“…A battery of highly sensitive immunodiagnostic techniques have been applied to detect specific antibody response in NCC patients. Taenia solium (Tso) cysticerci crude antigen and antigen B have frequently led to low specificity due to cross reactions (Espinoza et al 1986;Estrada et al 1989;Katti and Chandramukhi 1991;Malla et al 1992;Das et al 2002;Mandal et al 2006) and use of lower molecular mass (LMM) antigen fractions are reported to have high specificity (Grogl et al 1985;Gottstein et al 1986;Tsang et al 1989;Kaur et al 1995;Kaur et al 1996;Lissiane et al 1999)). In our earlier study, in adults, detection of antibody response to LMM antigen fraction (20-24 kDa) of Tso cysticerci, by ELISA had shown a sensitivity and specificity of 95.23% and 100%, respectively (Kaur et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

et al. 2008

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

et al. 2008

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“…Similar observations have been reported by other workers with CSF samples from patients with tuberculous meningitis. [24]…”

Section: Discussionmentioning

confidence: 99%

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Biswas

Parija

2011

Trop Parasitol

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Background:Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases.Aims:The aim of the present study was to standardize and evaluate the latex agglutination test (LAT) for the detection of Taenia solium metacestode antigen in the cerebrospinal fluid (CSF) and serum for the diagnosis of neurocysticercosis (NCC).Settings and Design:The study was conducted at Department of Microbiology, Jawaharlal Institute of Post graduate medical education and research after obtaining informed consent from the study subjects.Materials and Methods:In the present study, CSF and serum samples were collected from clinically suspected NCC, CT/MRI proven cases of NCC, non-cysticercal central nervous system infection control and from healthy control subjects. CSF was not collected from healthy controls. Polyclonal antisera raised in rabbits against porcine T. solium metacestode complete homogenate antigen, was used in the LAT to detect the antigen in the specimens.Statistical Analysis Used:The statistical analysis was carried out using Epi Info. The sensitivity, specificity, positive predictive value, and negative predictive value of the LAT were calculated.Results:The LAT exhibited sensitivity of 64.7% and specificity of 85.7% with CSF samples and sensitivity of 52.08% and specificity of 96% with serum samples.Conclusions:Results of the present study shows that the LAT can be employed as a moderately sensitive and specific test for the detection of T. solium metacestode antigen in the CSF and serum specimens for the diagnosis of NCC in poorly equipped laboratories.

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“…Because the immune response to various cysticercal antigens (CF, scolex, membrane, antigen B, immunoaffinity-purified parasite antigens, and nonruptured whole C. cellulosae from human and porcine sources) has been found to be heterogeneous, and certain immunodiagnostic parasitic antigens are exclusive to CF, scolex, and membrane [9,10], use of the 10-kDa antigen alone in immunodiagnosis of NCC could possibly yield a false-negative result. Therefore, ideally a homogenate of nonruptured whole metacestodes of Taenia solium should be used for further purification of parasitic antigens, as it contains all the components of different anatomical parts of the cyst [9].…”

Section: Assessment Of Specificity Of a Recombinant 10-kda Protein Anmentioning

confidence: 99%

“…Therefore, ideally a homogenate of nonruptured whole metacestodes of Taenia solium should be used for further purification of parasitic antigens, as it contains all the components of different anatomical parts of the cyst [9]. Earlier, antigenic differences between C. cellulosae of porcine and human sources and antigenic variations in C. cellulosae obtained from infested pigs from different regions of the Indian subcontinent were observed [11,12].…”

Section: Assessment Of Specificity Of a Recombinant 10-kda Protein Anmentioning

confidence: 99%

Assessment of Specificity of a Recombinant 10‐kDa Protein Antigen in Differential Diagnosis of Neurocysticercosis

Katti

2000

J INFECT DIS

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mydia pneumoniae stimulate cytokine production in human blood mononuclear cells. Eur J Immunol 2000; 30:541-9 . 4. Kol A, Sukhova G, Lichtman A, Libby P. Chlamydial heat shock protein 60 localizes in human atheroma and regulates macrophage tumor necrosis factor-alpha and matrix metalloproteinase expression. Circulation 1998; 98:300-7. 5. Kol A, Bourcier T, Lichtman AH, Libby P. Chlamydial and human heat shock protein 60s activate human vascular endothelium, smooth muscle cells, and macrophages. J Clin Invest 1999; 103:571-7. 6. Kalayoglu MV, Byrne GI. Induction of macrophage foam cell formation by Chlamydia pneumoniae. J Infect Dis 1998; 177:725-9. 7. Kalayoglu MV, Byrne GI. A Chlamydia pneumoniae component that induces macrophage foam cell formation is chlamydial lipopolysaccharide. Infect Immun 1998; 66:5067-72. 8. Kalayoglu MV, Miranpuri GS, Golenbock DT, Byrne GI. Characterization of low density lipoprotein uptake by murine macrophages exposed to Chlamydia pneumoniae. Microbes Infect 1999; 6:409-18. 9. Leeuwenburgh C, Rasmussen JE, Hsu FF, Mueller DM, Pennathur S, Heinecke JW. Mass spectrometric quantification of markers for protein oxidation by tyrosyl radical, copper, and hydroxyl radical in low density lipoprotein isolated from human atherosclerotic plaques.

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Comparative evaluation of cysticercal antigens and immunoassays in the diagnosis of neurocysticercosis

Cited by 18 publications

References 10 publications

Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Assessment of Specificity of a Recombinant 10‐kDa Protein Antigen in Differential Diagnosis of Neurocysticercosis

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