Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

Mandal, Jharna; Singhi, Pratibha; Khandelwal, Niranjan; Malla, Nancy

doi:10.1007/s00436-008-0933-4

Cited by 14 publications

(13 citation statements)

References 28 publications

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“…Several authors tested different antigens, purified or recombinant, with different indexes of sensitivity and specificity [6,20,22,23,34,35]. The sensitivities and specificities data with the phage clones obtained here were similar or, in several cases, better, that the ones obtained from other antigens mentioned above.…”

Section: Discussionsupporting

confidence: 62%

Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

et al. 2010

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Section: Discussionsupporting

confidence: 62%

Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

et al. 2010

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“…Data obtained from cerebrospinal fluid (CSF), including immunological tests based on antibody detection, are valuable and have been used for many decades to diagnose NC 23,24 . Taenia antigen (TA) detection using the ELISA technique [25][26][27][28] , is a more recent method that can provide additional information in NC diagnosis. Moreover, TA detection allows recognition of NC clinical activity and may be useful in the follow-up of these patients [29][30][31] .…”

mentioning

confidence: 99%

Neurocysticercosis: relationship between Taenia antigen levels in CSF and MRI

Abraham

Livramento

Leite³

et al. 2010

Arq. Neuro-Psiquiatr.

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Objective: To determine the relationship between Taenia antigen (TA) detection in cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI) findings in patients with definite diagnosis of neurocysticercosis (NC). Method: Sixty-three patients with definite diagnosis of NC were submitted to a MRI of the brain, and to a CSF examination, with a meticulous search for TA by ELISA. results: TA detection was positive in 36 patients (57.1%). A total of 836 lesions were analyzed, greatly within the cerebral parenchyma (98.7 of the lesions). Intact or non-degenerating cysts were the most common evolutive phase observed (50.4% of all lesions), 22.1% were degenerating cysts and 19.5% calcified cysts. We observed a significant relationship between TA levels detected and the total number of lesions and the number of non-degenerating cysts, but not with calcified lesions. conclusion: According to our results, we propose at least four important types of contribution: (1) TA detection may allow etiologic diagnosis in transitional phases of NC, with non-characteristic images; (2) in final stages of evolution of cysticercoids in the CNS, lesions may not appear on CT or MRI, and TA detection may contribute to a definite etiologic diagnosis; (3) TA detection may permit diagnosis of NC in some patients with previous negative tests for antibody detection in CSF; (4) TA detection may represent an accurate marker of disease activity in the epileptic form of NC. Key words: central nervous system, cerebrospinal fluid, enzyme-linked immunosorbent assay, magnetic resonance imaging, neurocysticercosis, Taenia antigens detection.Neurocisticercose: relação entre antígeno da Taenia no líquido cefalorraquidiano e ressonância magnética resuMO Objetivo: Determinar a relação entre a detecção de antígeno de Taenia (TA) no líquido cefalorraquidiano (LCR) e achados de ressonância magnética (RM) em pacientes com diagnóstico definitivo de neurocisticersose. Método: Sessenta e três pacientes com diagnóstico de NC foram submetidos a exame de RM e exame de LCR com pesquisa de antígeno de Taenia por método imunoenzimático. resultados: A detecção de TA foi positiva em 36 pacientes (57,1%). Um total de 836 lesões foram analizadas sendo 98,7% intraparemquimatosas, 50,4% dos cistos encontravam-se íntegros, 22,1% degenerados e 19,5% calcificados. Foi observada relação significativa entre a presença dos níveis de TA detectados com o número total dos cistos e também com o número de cistos íntegros. Não foi observada relação com cistos calcificados. conclusão: (1) a detecção de TA permite o diagnóstico etiológico em formas transicionais na NC com imagem pouco característica; (2) em estágio evolutivo final de um cisticerco no sistema nervoso, este pode não aparecer na tomografia computadorizada ou RM sendo a presença do antígeno importante para confirmação diagnóstica; (3) a detecção do TA permite também o diagnóstico de NC nos casos em que as reações inumológicas são negativas; (4) a detecção do TA representa um marcador de atividade da doença nas forma...

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“…Another disadvantage is that cross-reactivity may occur with other parasitic diseases, most commonly Echinococcus granulosus [25, 62–67], although cross-reactivity has also been reported with other diseases as hymenolepiasis, fasciolasis [62], toxoplasmosis [67, 68], malaria [67], amoebiasis [67], syphilis [68], hepatitis B [68], toxocariasis [62], cerebral tuberculosis [37, 67], mononucleosis [68], among others [25, 37, 65], which may be due to the selection of bad characterized samples and not necessarily to the fact that patients are infected with something else than T. solium . However, the search for antibodies in samples has the advantage that mixtures of antigens can be used (as parasite extracts or semipurified antigens) while the search for antigens needs to have specific antibodies against the desired antigens but can indicate an active infection [15, 69].…”

Section: Detection Of Antigens or Antibodies For The Immunodiagnosmentioning

confidence: 99%

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

Esquivel-Velázquez¹,

Ostoa‐Saloma²,

Morales‐Montor³

et al. 2011

BioMed Research International

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Neurocysticercosis (NCC) is a disease of the central nervous system that is considered a public health problem in endemic areas. The definitive diagnosis of this disease is made using a combination of tools that include imaging of the brain and immunodiagnostic tests, but the facilities for performing them are usually not available in endemic areas. The immunodiagnosis of NCC is a useful tool that can provide important information on whether a patient is infected or not, but it presents many drawbacks as not all infected patients can be detected. These tests rely on purified or semipurified antigens that are sometimes difficult to prepare. Recent efforts have focused on the production of recombinant or synthetic antigens for the immunodiagnosis of NCC and interesting studies propose the use of new elements as nanobodies for diagnostic purposes. However, an immunodiagnostic test that can be considered as “gold standard” has not been developed so far. The complex nature of cysticercotic disease and the simplicity of common immunological assumptions involved explain the low scores and reproducibility of immunotests in the diagnosis of NCC. Here, the most important efforts for developing an immunodiagnostic test of NCC are listed and discussed. A more punctilious strategy based on the design of panels of confirmed positive and negative samples, the use of blind tests, and a worldwide effort is proposed in order to develop an immunodiagnostic test that can provide comparable results. The identification of a set of specific and representative antigens of T. solium and a thorough compilation of the many forms of antibody response of humans to the many forms of T. solium disease are also stressed as necessary.

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Evaluation of lower molecular mass (20–24 kDa) Taenia solium cysticercus antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children

Cited by 14 publications

References 28 publications

Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

Neurocysticercosis: relationship between Taenia antigen levels in CSF and MRI

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

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