Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

Ribeiro, Vanessa da Silva; Manhani, Marianna Nascimento; Cardoso, Rafael M.; Vieira, Carlos Ueira; Goulart, Luiz Ricardo; Costa-Cruz, Júlia Maria

doi:10.1016/j.imlet.2010.01.008

Cited by 24 publications

(19 citation statements)

References 31 publications

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“…89 Initial promising results have been reported for two other peptides produced with this technique. 90,91 As proposed by EsquivelVelazquez et al, in 2011, new tools like phage display peptide selection, production of synthetic, and recombinant antigens, could permit us to shorten the path to identify specific antigens capable to distinguish not only the stages of the parasite, but also exposure from viable and non-viable infection. 92 In this way, a good alternative to distinguish exposure from infection could be the use of oncospheral antigens, which to date have mostly been used as vaccine candidates.…”

Section: -24mentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

126

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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Section: -24mentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

126

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“…Since its first description (36) and introduction into laboratory practice (37), phage display technology has proven to be useful in selecting specific peptides that are highly reactive against many pathogen targets. Its biological applications have ranged from use in studies of vaccine candidates for rabies (38), hepatitis C (39), E. coli (40), Plasmodium vivax (23), and Schistosoma japonicum (41) to use with diagnostic antigens for the detection of antibodies to pneumonia (42), neurocysticercosis (43)(44)(45), equine viral arteritis (46), rabies (47), and anaplasmosis (48). These phage-displayed peptides, also called mimotopes, have shown either linear or conformational recognition with high affinity to paratope sequences of antibodies.…”

Section: Discussionmentioning

confidence: 99%

Subtractive Phage Display Selection from Canine Visceral Leishmaniasis Identifies Novel Epitopes That Mimic Leishmania infantum Antigens with Potential Serodiagnosis Applications

Costa¹,

Lima²,

Chávez-Fumagalli³

et al. 2013

Clin. Vaccine Immunol.

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i Visceral leishmaniasis (VL) is a zoonotic disease that is endemic to Brazil, where dogs are the main domestic parasite reservoirs, and the percentages of infected dogs living in regions where canine VL (CVL) is endemic have ranged from 10% to 62%. Despite technological advances, some problems have been reported with CVL serodiagnosis. The present study describes a sequential subtractive selection through phage display technology from polyclonal antibodies of negative and positive sera that resulted in the identification of potential bacteriophage-fused peptides that were highly sensitive and specific to antibodies of CVL. A negative selection was performed in which phage clones were adhered to purified IgGs from healthy and Trypanosoma cruzi-infected dogs to eliminate cross-reactive phages. The remaining supernatant nonadhered phages were submitted to positive selection against IgG from the blood serum of dogs that were infected with Leishmania infantum. Phage clones that adhered to purified IgGs from the CVL-infected serum samples were selected. Eighteen clones were identified and their reactivities tested by a phage enzyme-linked immunosorbent assay (phage-ELISA) against the serum samples from infected dogs (n ‫؍‬ 31) compared to those from vaccinated dogs (n ‫؍‬ 21), experimentally infected dogs with cross-reactive parasites (n ‫؍‬ 23), and healthy controls (n ‫؍‬ 17). Eight clones presented sensitivity, specificity, and positive and negative predictive values of 100%, and they showed no crossreactivity with T. cruzi-or Ehrlichia canis-infected dogs or with dogs vaccinated with two different commercial CVL vaccines in Brazil. Our study identified eight mimotopes of L. infantum antigens with 100% accuracy for CVL serodiagnosis. The use of these mimotopes by phage-ELISA proved to be an excellent assay that was reproducible, simple, fast, and inexpensive, and it can be applied in CVL-monitoring programs.

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“…Phage-ELISA was performed as described previously [35] with modifications for validation of selected phage clones. Preliminary experiments were conducted to determine the optimal conditions for ELISA by titrating reagents (phages, sera and conjugate).…”

Section: Methodsmentioning

confidence: 99%

“…Selected mimotopes may be used in a simple, specific and low cost phage ELISA as described by several authors [31]–[35]. The aim of this study was to develop novel antigens for serodiagnosis of S. stercoralis with improved sensitivity and specificity, and reduced cross-reactions with other parasite infections, a major problem when crude antigens are used.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage-Fused Peptides for Serodiagnosis of Human Strongyloidiasis

et al. 2014

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BackgroundStrongyloidiasis, a human intestinal infection caused by the nematode Strongyloides stercoralis, is frequently underdiagnosed and although its high prevalence is still a neglected parasitic disease because conventional diagnostic tests based on parasitological examination (presence of Strongyloides larvae in stool) are not sufficiently sensitive due to the low parasitic load and to the irregular larval output. There is an urgent need to improve diagnostic assays, especially for immunocompromised patients with high parasitic load as consequence of self-infection cycle, which can disseminate throughout the body, resulting in a potentially fatal hyperinfection syndrome often accompanied by sepsis or meningitis.Methods/Principal FindingsWe have performed Phage Display technology to select peptides that mimic S. stercoralis antigens, capable of detecting a humoral response in patients with strongyloidiasis. The peptides reactivity was investigated by Phage-ELISA through different panels of serum samples. We have successfully selected five peptides with significant immunoreactivity to circulating IgG from patients' sera with strongyloidiasis. The phage displayed peptides C9 and C10 presented the highest diagnostic potential (AUC>0.87) with excellent sensitivity (>85%) and good specificity (>77.5%), suggesting that some S. stercoralis antigens trigger systemic immune response.Conclusions/SignificanceThese novel antigens are interesting serum biomarkers for routine strongyloidiasis screenings due to the easy production and simple assay using Phage-ELISA. Such markers may also present a promising application for therapeutic monitoring.

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Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

Cited by 24 publications

References 31 publications

Immunological and molecular diagnosis of cysticercosis

Immunological and molecular diagnosis of cysticercosis

Subtractive Phage Display Selection from Canine Visceral Leishmaniasis Identifies Novel Epitopes That Mimic Leishmania infantum Antigens with Potential Serodiagnosis Applications

Bacteriophage-Fused Peptides for Serodiagnosis of Human Strongyloidiasis

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