Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study

Vanapatla, Amulya; Vemisetty, Harikumar; Punna, Rajani; Veeramachineni, Chandrasekhar; Venkata, Ravichandra Polavarapu; Muppala, Jaya Nagendra Krishna; Dandolu, Ramkiran

doi:10.7860/jcdr/2016/17228.7645

Cited by 6 publications

(13 citation statements)

References 19 publications

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“…Since ALP at alkaline pH releases inorganic phosphate [17,41], researchers have suggested a pivotal role played by this enzyme in the early stages of hard tissue formation. Considering that Apexit Plus and Sealapex release hydroxyl and provide an alkaline pH to the medium [11,31], it is not surprising that these sealers stimulate the ALP expression by cells of the subcutaneous connective tissue, as observed in the present study. This evidence is confirmed by the fact that immunostained cells were not seen in the connective tissue of the control group, except in vascular cells.…”

Section: Biological Propertiessupporting

confidence: 77%

“…The base paste of Apexit Plus contains calcium hydroxide, calcium oxide, hydrated collophonium, highly dispersed silicon dioxide, and alkyl ester of phosphoric acid, while the activator paste contains disalicylate, bismuth hydroxide, bismuth carbonate associated to silicon dioxide, and phosphoric acid alkyl ester [4,9]. This calcium hydroxide-based sealer has caused slight cytotoxicity when cultured with L929 fibroblasts [10] and exerted good antimicrobial activity [11]. It has been suggested that the high pH provided by this sealer (to above 12.5) may be responsible for its antimicrobial effect [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…This calcium hydroxide-based sealer has caused slight cytotoxicity when cultured with L929 fibroblasts [10] and exerted good antimicrobial activity [11]. It has been suggested that the high pH provided by this sealer (to above 12.5) may be responsible for its antimicrobial effect [11,12]. Root canal filling materials should present some favorable psychochemical properties [6][7][8], including sufficient radiopacity to be distinguished from bone and teeth and an adequate flow capacity to be inserted into the root canal system [6].…”

Section: Introductionmentioning

confidence: 99%

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Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

et al. 2020

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We investigated the physical properties, antimicrobial activity, and tissue reaction to Apexit Plus in comparison to Sealapex. Flow, radiopacity, setting time, and solubility were evaluated in each material. The antimicrobial activity against Enterococcus faecalis was performed. Polyethylene tubes containing Apexit Plus or Sealapex, and without material (control group) were implanted into the subcutaneous tissue of rats. At 7, 15, 30, and 60 days of implantation, the specimens were paraffin-embedded and the number of inflammatory cells (ICs) and the amount of birefringent collagen (BC) were quantified. The von Kossa reaction followed by immunohistochemistry for detection of alkaline phosphatase (ALP) was also performed. Statistical analysis was performed with ANOVA and Tukey test (p ≤ 0.05). The flow value of Apexit Plus was greater than Sealapex, whereas the radiopacity (3.44 mm Al) was lower than Sealapex (6.82 mm Al). Apexit Plus showed lower solubility and shorter initial and final setting (p < 0.0001), whereas the antimicrobial activity was significantly greater than Sealapex. Although the number of ICs was higher in Apexit Plus (p = 0.0009) at 7 days, no significant difference was detected between Apexit Plus and Sealapex at 15, 30, and 60 days. All groups showed higher values for BC in the capsules over time. ALP-immunolabelled cells were observed, mainly around von Kossa-positive structures, either in the capsules of Apexit Plus or Sealapex. Therefore, our results revealed that Apexit Plus exhibited a greater effectiveness against Enterococcus faecalis and better physical properties than Sealapex, except for the radiopacity. In vivo findings indicate that Apexit Plus is biocompatible and presents potential bioactivity in the subcutaneous tissue.

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Section: Biological Propertiessupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

et al. 2020

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“…Devido à complexidade anatômica do sistema de canais radiculares e fatores de virulência, os micro-organismos adquirem resistência a soluções irrigantes, medicamentos intra-canal e compostos antimicrobianos, ocasionando reinfecções subsequentes (Paz, 2007;Wu et al, 2014;Al-Shwaimi et al, 2016;Vanapatla et al, 2016).…”

Section: Introdução -25unclassified

“…Assim, pode-se encontrar na literatura estudos que avaliaram a atividade antimicrobiana de cimentos endodônticos comerciais, puros e com a incorporação de agentes antimicrobianos, frente a diversas espécies de micro-organismos, como o Streptococcus mutans, Lactobacillus casei e Actinomyces viscosus, utilizados para avaliar o efeito inibidor dos cimentos RoekoSeal, Endomethasone N, N2, Apexit Plus e AH Plus, incorporados com 2% de cloreto de benzalcônio e 2% de cloreto de cetilpiridínio, pelo método de difusão em ágar (Gjorgievskaa et al, 2013). Leonardo et al (2000) avaliaram o efeito inibidor de cimentos endodônticos (Sealapex, Fill Canal, Ketac Endo, AH Plus), pelo método de difusão em ágar, frente a sete espécies de micro-organismos, sendo cinco Gram-positivos: No entanto, a maioria dos estudos encontrados na literatura avaliaram o efeito antimicrobiano de cimentos endodônticos frente a Enterococcus faecalis (Rezende et al, 2016;Shakya et al, 2016;Wu et al, 2016;Vanapatla et al, 2016;Carpio-Perochena et al, 2015;Wang;Haapasalo, 2014;Baer;Maki, 2010;Hiraishi et al, 2009;Zhang et al, 2009;Slutzky-Goldberg et al, 2008;Hoelscher;Bahcall;Maki, 2006;Çobankara et al, 2004;Mickel;Nguyen;Chogle, 2003), uma vez que há relatos que esse micro-organismo é o mais prevalente em canais infectados e em casos de retratamentos; sua prevalência varia de 24% a 77% (Al-Shwaimi et al, 2016).…”

Section: Introdução -25unclassified

Cimento endodôntico antimicrobiano com a incorporação de vanadato de prata - análise das propriedades físicas e microbiológicas

Teixeira¹

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À minha orientadora Profa. Dra. Andréa Cândido dos Reis por estar sempre presente preocupando-se com a minha formação, futuro e felicidade. Agradeço não somente aos anos de orientação, mas também a amizade, paciência, encorajamento em momentos de incerteza, ensinamentos que contribuíram para minha formação profissional e pessoal, e por acreditar nas minhas capacidades. Sem você nada disso seria possível!

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Antimicrobial Activity, Physical Properties and Sealing Ability of Epoxy Resin-Based Sealer Impregnated with Green Tea Extract-Chitosan Microcapsules: An In-Vitro Study

Habib

Sadek

Hasanin

et al. 2021

Egyptian Dental Journal

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Aim: To assess the effect of prepared green tea-chitosan microcapsules on the antibacterial efficiency against Enterococcus faecalis, the physical properties, and the sealing ability of resinbased endodontic sealer impregnated with these microcapsules. Materials and Methods:Green tea extract (GTE) and chitosan solution were prepared, then GTE was dissolved into this solution followed by precipitation of chitosan nanoparticles (CSNPs) loaded with GTE. Microcapsulation of GTE-CSNPs was performed and the solution was prepared (3 % w/v concentration). Characterization of microcapsules was conducted using Fourier-Transform Infrared Spectroscopy (FTIR), Zeta potential, and transmission electron microscope (TEM). The release of GTE at different pH values was evaluated. GTE-CSNPs solution was added to an epoxy resin-based sealer and the antibacterial activity was evaluated via direct contact test. The physical properties of the modified sealer were evaluated according to American Dental Association (ADA) specifications. Sealing ability was evaluated stereomicroscopically. Data were statistically analyzed.Results: FTIR and Zeta potential ensured the attachment between GTE and Chitosan. The particle size of CSNPs and GTE-CSNPs was around 20 nm, while microcapsules were greater than 1000 nm. A significantly higher percentage of GTE was released at pH 5 than pH 7.4, (P < 0.001). After sealer modification, the antibacterial evaluation showed significant decrease in optical density (P < 0.001). Also, the setting time, film thickness and microleakage were significantly decreased, however, the solubility was significantly increased (P < 0.001) but still within the acceptable limit of ADA specifications.Conclusions: GTE-Chitosan microcapsule is a proper natural candidate for improvement of sealer's properties.

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Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study

Cited by 6 publications

References 19 publications

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Cimento endodôntico antimicrobiano com a incorporação de vanadato de prata - análise das propriedades físicas e microbiológicas

Antimicrobial Activity, Physical Properties and Sealing Ability of Epoxy Resin-Based Sealer Impregnated with Green Tea Extract-Chitosan Microcapsules: An In-Vitro Study

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