Comparative efficacy evaluation of seven commercial injectable ceftriaxone purchased at Ambo, Oromia Regional State, Ethiopia, using agar well plate diffusion method

Abstract

Background Irrational use of this drug leads to emergence of resistant bacteria and also leads to infections that are worse than the original diagnosed ones. Important concern is the uncertainty of administered antibiotics about whether the quality of a generic medicine is equal to brand name drug; if both brand/generic are bioequivalent, then antibiotic selection should be based on the cost of therapy. Methods The efficacy of seven ceftriaxone brands available at Ambo, Oromia Regional State, Ethiopia, was evaluated by microwell plate diffusion technique against four different bacteria. Results All brands of two concentrations were showed sufficient inhibitory activity against four microorganisms, so they should all be considered as bioequivalence. Among all brands tested, B6 showed higher activity against Escherichia coli American Type Culture Collection (ATCC) 10536, Staphylococcus aureus ATCC 29737 and Pseudomonas aeruginosa ATCC 25619, and B1 showed higher activity against Salmonella typhi ATCC 06775. Interestingly, the concentration C2 (50 μg/ml) of B1-B7 showed equivalent zone of inhibition to that of the standard. ConclusionWe conclude that performance of tested ceftriaxone injectable products were equivalent to the standard. We recommend that the physicians may select the ceftriaxone brand which is low cost in order to reduce the cost of treatment.

Evaluation of in-vivo and in-vitro microbiological methods for testing the efficacy of parenteral antibiotics: A Review

“…These researchers compared the diameters of the zone of inhibition between different brands of antibiotics to determine efficacy. Gunasekaran et al 22 tested seven parenteral ceftriaxone brands using this technique. Diluted test antibiotic solutions were added to wells punched in the agar inoculated with the control bacterial strains and allowed to diffuse.…”

Ceftriaxone Injectables: An In vitro Evaluation of Antimicrobial Activity and Comparative Efficacy

“…Many generics of ceftriaxone fail to match the branded product's quality criteria, according to past studies in Japan and England. Another trial found a decrease in efficacy and an increased probability of clinical failure [16][17][18]. Study in Ethiopia, on the other hand, found that the efficacy of generic brands of Ceftriaxone was equivalent to that seen in the parent version of the drug [19].…”