Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

Abstract

Abstract:The effects of amidated pectin and psyllium on serum, hepatic and faecal cholesterol concentration were compared in female rats fed diets supplemented with palm fat and cholesterol at 50 and 10 g/kg, respectively. Control rats were fed a diet supplemented with cellulose at 60 g/kg. In treated rats, cellulose was replaced with either amidated pectin or psyllium. Amidated pectin and psyllium intake significantly decreased serum cholesterol from 3.41 µmol/ml (control) to 1.68 and 2.04 µmol/ml, respectively, and hepatic cholesterol from 31.9 µmol/g (control) to 7.2 and 9.0 µmol/g, respectively. Histology with lipid-staining Sudan Black B revealed that liver tissue from control rats was infiltrated with lipids, but staining was absent in livers of treated rats. No hepatic pathophysiology was apparent in treated rats. Amidated pectin and psyllium intake significantly increased faecal fat content. Faecal cholesterol content was significantly increased in rats that were fed amidated pectin, and non-significantly increased in rats that were fed psyllium. Body weight and food intake did not differ among treatment groups. In conclusion, amidated pectin, a novel sequestrant of sterols, demonstrated a similar effect on rat serum and hepatic cholesterol concentration to psyllium, which is a well-established hypocholesterolaemic agent.© Versita Sp. z o.o.

‘Saba’ banana (Musa acuminata x balbisiana BBB Group) peel pectin supplementation improves biomarkers of obesity and associated blood lipid disorders in obese hypercholesterolemic mice

“…These properties of pectin help in bile acid excretion and reduction in bile acid reabsorption ( Anderson et al, 2009 ; Gunness and Gidley, 2010 ), leading to lower serum TC and LDL-C concentrations ( Wahlström et al, 2016 ). The observed effect of SPP on blood TC was comparable with the values obtained from hawthorn ( Zhu et al, 2015 ), apple ( Sanchez et al, 2008 and Adam et al, 2015a ), and citrus pectin ( Marounek et al, 2010a and Marounek et al, 2010b ). Meanwhile, the observed decreased effect of SPP at 9th week could possibly be due to the aging-induced changes in cholesterol metabolism of mice such as increased adipose over muscle mass, increased insulin resistance, and decreased breakdown of cholesterol to bile acids ( Uranga and Keller, 2010 ).…”

Preparation of amidated derivatives of carboxymethylcellulose

“…They are associated with many biological activities and thus can be used as therapeutic agents. On the other hand, hydrophobic polysaccharide derivatives are interesting for cholesterol and fat lowering [21,[35][36][37][38]. In another our work [39] N-octadecylamides of both MCC and CMC were tested as lipid sorbents.…”

Gender-based differences in the effect of dietary cholesterol in rats

“…In recent studies, rats were used to investigate the hypocholesterolemic effects of soluble and insoluble fibre [1], plant proteins [2,3], chitosan [4], algae Chlorella [5], α-ketoglutarate [6], aluminosilicates [7], amidated pectin [8], and interesterified oils [9]. Compared to guinea pigs [10] and hamsters [11], rats are less susceptible to hypercholesterolaemia induction by dietary cholesterol, presumably because the high activity of hepatic cholesterol 7α-hydroxylase allows these rodents to convert excess dietary cholesterol to bile acids [10].…”