Gender-based differences in the effect of dietary cholesterol in rats

Marounek, M.; Volek, Zdeněk; Skřivanová, E.; Czauderna, M.

doi:10.2478/s11535-012-0091-7

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…However, it was shown that different physiological conditions such as gender and age could affect a dysregulation in brain cholesterol metabolism [22,23]. In addition, cholesterol concentration in serum significantly differed in male and female rats fed the cholesterol-containing diet [24]. Thus, further experiments will be needed to compare the effect of a high-cholesterol diet on PLCβ1 expression and PIP 2 levels by gender and age.…”

Section: Discussionmentioning

confidence: 99%

High-Cholesterol Diet Decreases the Level of Phosphatidylinositol 4,5-Bisphosphate by Enhancing the Expression of Phospholipase C (PLCβ1) in Rat Brain

Chun

Chung

2020

IJMS

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Cholesterol is a critical component of eukaryotic membranes, where it contributes to regulating transmembrane signaling, cell–cell interaction, and ion transport. Dysregulation of cholesterol levels in the brain may induce neurodegenerative diseases, such as Alzheimer’s disease, Parkinson disease, and Huntington disease. We previously reported that augmenting membrane cholesterol level regulates ion channels by decreasing the level of phosphatidylinositol 4,5-bisphosphate (PIP2), which is closely related to β-amyloid (Aβ) production. In addition, cholesterol enrichment decreased PIP2 levels by increasing the expression of the β1 isoform of phospholipase C (PLC) in cultured cells. In this study, we examined the effect of a high-cholesterol diet on phospholipase C (PLCβ1) expression and PIP2 levels in rat brain. PIP2 levels were decreased in the cerebral cortex in rats on a high-cholesterol diet. Levels of PLCβ1 expression correlated with PIP2 levels. However, cholesterol and PIP2 levels were not correlated, suggesting that PIP2 level is regulated by cholesterol via PLCβ1 expression in the brain. Thus, there exists cross talk between cholesterol and PIP2 that could contribute to the pathogenesis of neurodegenerative diseases.

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Section: Discussionmentioning

confidence: 99%

High-Cholesterol Diet Decreases the Level of Phosphatidylinositol 4,5-Bisphosphate by Enhancing the Expression of Phospholipase C (PLCβ1) in Rat Brain

Chun

Chung

2020

IJMS

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“…The RNA isolation and real-time PCR assay were performed as described previously (Marounek et al 2012). Briefly, total RNA was extracted from the liver tissue using an RNeasy Mini Kit (Qiagen, USA), reverse transcribed, and PCR was performed using the QuantiTect SYBR Green RT-PCR Kit (Qiagen, USA).…”

Section: Rna Isolation and Real-time Pcr Assaymentioning

confidence: 99%

Comparative Study on the Hypocholesterolemic Activity of Amidated Polysaccharides and Psyllium

et al. 2015

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The effects of amidated carboxymethylcellulose, amidated pectin, and psyllium on serum and hepatic cholesterol, hepatic fat, and fecal output of sterols were examined in female rats. Rats were fed a diet supplemented with cholesterol (0 or 10 g/kg) and palm fat. Amidated cellulose at 30 g/kg significantly decreased the serum and hepatic concentration of cholesterol by 28.1% and 64.6%, respectively. Corresponding values in rats fed amidated pectin were 28.9% and 72.4%. The effects of psyllium were similar, but less pronounced. Amidated pectin significantly increased the fecal output of cholesterol, total neutral sterols, and total sterols by 49.1%, 31.9%, and 31.0%, respectively. Amidated cellulose and psyllium increased the fecal excretion of total sterols by 1.1% and 5.5%, respectively. In the feces of rats fed amidated cellulose, a small amount of conjugated bile acids was detected (0.83% of total bile acids). In these rats, the lowest expression of hepatic cholesterol 7α-hydroxylase was detected, corresponding to the low fecal output of bile acids. We conclude that the hypocholesterolemic effects of both amidated polysaccharides were similar in spite of their different affinity to sterols.

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“…Analyses of the feed were performed as described previously (Marounek et al 2012). The serum levels of total cholesterol, LDL cholesterol, triacylglycerols, and aminotransferases were determined using commercial kits supplied by Erba-Lachema, Ltd. (Brno, Czech Republic).…”

Section: Chemical Analysesmentioning

confidence: 99%

Hydrophobically Modified Celluloses as Novel Cholesterol-lowering Polymers

Tůma¹,

Volek²,

Synytsya³

et al. 2014

BioResources

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Hydrophobically modified celluloses were prepared by the esterification of monocarboxycellulose (MCC) and carboxymethylcellulose (CMC) with methanol, followed by the amino-de-alkoxylation of the methyl esters with n-octadecylamine. These cellulose derivatives were fed at 30 and 60 g/kg for 4 weeks to female rats. The diets were supplemented with palm fat (60 or 50 g/kg) and cholesterol (0 or 10 g/kg). There was no significant effect of amidated celluloses on the feed intake or body weight of rats. Both MCC and CMC derivatives significantly decreased the concentrations of cholesterol present in the serum and liver. The supplementation of diets with hydrophobically modified celluloses tended to increase fecal concentrations of cholesterol and coprostanol and significantly increased fecal concentrations of total neutral sterols. In rats fed diets containing cholesterol, the total serum cholesterol correlated negatively with the fecal concentrations of neutral sterols. It can be concluded that MCC-C18 and CMC-C18 are effective cholesterollowering agents.

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Gender-based differences in the effect of dietary cholesterol in rats

Cited by 6 publications

References 26 publications

High-Cholesterol Diet Decreases the Level of Phosphatidylinositol 4,5-Bisphosphate by Enhancing the Expression of Phospholipase C (PLCβ1) in Rat Brain

High-Cholesterol Diet Decreases the Level of Phosphatidylinositol 4,5-Bisphosphate by Enhancing the Expression of Phospholipase C (PLCβ1) in Rat Brain

Comparative Study on the Hypocholesterolemic Activity of Amidated Polysaccharides and Psyllium

Hydrophobically Modified Celluloses as Novel Cholesterol-lowering Polymers

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