Comparative drug sensitivities of culture forms of Trypanosoma cruzi and Trypanosoma dionisii

Gaborak, Maria; Darling, John L.; Gutteridge, W. E.

doi:10.1038/268339a0

Cited by 7 publications

(4 citation statements)

References 4 publications

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“…It can be seen that, for all assayed drugs occurred a remarkable dose-dependent inhibitory effect reaching practically to a total parasite growth inhibition, at the end of the incubation time. Those results are agreements with the GABORAK et al 3 observation, and we can conclude that the trypanosome used appear to be a suitable model for the preliminary screen of anti-T. cruzi drugs, mainly in less-equipped laboratory in developing countries.…”

Section: T (S) Cruzi Growth Inhibition Experiments Were Carried Out supporting

confidence: 81%

“…In order to avoid handling the pathogenic T. cruzi for preliminary screening of anti-T. cruzi organic compounds GABORAK et al 3 , had earlier suggested the use of other members of the Schizotrypanum genus instead of, since a number of compounds of known anti-T. (S.) cruzi activity had showed similar activity when tested in vitro against nonpathogenic trypanosomes.…”

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confidence: 99%

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Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae)

Duarte¹,

Filho²,

Silva³

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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SUMMARYFour pentacyclic triterpenes isolated from Austroplenckia populnea and four compounds of known anti T. cruzi or anti-malarial activity were tested. Of those triterpenes tested 20a-hydroxy-tingenone showed high activity, epikatonic acid was less active, while populnilic and populninic acids were inactive against the trypanosome of the subgenus Schizotrypanum tested. Benzonidazole, nifurtimox, ketoconazole and primaquine presented a remarkable dose-dependent inhibitory effect reaching practically to a total growth inhibition of the parasite at the end of incubation time. The trypanosome tested appear to be a suitable model for preliminary screen for anti T. (S.) cruzi compounds.

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Section: T (S) Cruzi Growth Inhibition Experiments Were Carried Out supporting

confidence: 81%

mentioning

confidence: 99%

Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae)

Duarte¹,

Filho²,

Silva³

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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“…Both species share antigenic components and epitopes that are recognized by monoclonal antibodies against T. cruzi [19,20]. The data suggest that T. dionisii is a potential experimental model for initial screening and further biochemical studies related to the development of new drugs for the treatment of Chagas disease, as previously suggested by Gutteridge group in 1977 [21].…”

Section: Introductionsupporting

confidence: 59%

Trypanosoma dionisii as an experimental model to study anti-Trypanosoma cruzi drugs: A comparative analysis with benznidazole, posaconazole and amiodarone

Es¹,

Tr²,

W³

2018

Int J Clin Microbiol Biochem Technol

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Chagas disease is a public health problem in Latin America and its treatment is based on the use of benznidazole or nifurtimox compounds. Both present problems such as resistance, ineffi ciency in chronic infection and cytotoxic effects. New compounds such as posaconazole and amiodarone have been tested against T. cruzi and shown to be effective. In addition, new molecules have been synthesized and tested against T. cruzi. Because this protozoan is highly pathogenic, even with a number of cases of accidental laboratory infections, few laboratories located outside Latin America are authorized to work with its infective developmental stages. On the other hand, Trypanosoma dionisii is a non-pathogenic protozoan phylogenetically related to T. cruzi and that shares similar strategies to complete its life cycle in mammalian cells in vitro. Here, we describe a comparative analysis of the sensitivity of both parasites to benznidazole, posoconazole and amiodarone. We also analyzed the morphological effects of these compounds on both Trypanosoma species using electron microscopy. Our results show that T. dionisii is more sensitive to the compounds tested than T. cruzi. They also support a previous suggestion that it may constitute an excellent model for large scale screening of compounds against T. cruzi.

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“…Trypanosoma dionisii, however, failed to produce blood forms in chicken embryos, was only slightly infective to mouse fibroblasts and would not develop in chicken fibroblasts (Bower and Woo, unpublished data). Baker (1976) and Gaborak et al (1977) proposed using T . dionisii as a harmless laboratory model for studies relevant to T .…”

Section: Discussionmentioning

confidence: 99%

Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

Bower¹,

Woo²

1981

Can. J. Zool.

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Two new species of trypanosomes (subgenus Schizotrypanum) in bats fromsouthern Ontario. Can. J. Zool. 59: 530-545. From November of 1976 to May of 1979, the blood of 529 bats from 12 sites in southern Ontario was examined for trypanosomes using the haematocrit centrifugation technique. Trypanosoma hedricki n.sp. was found in 62 of 216 Eptesicus fuscus and Trypanosoma myoti n.sp. in 16 of 313 Myotis lucifugus. Blood forms of both species were morphologically similar to Trypanosoma cruzi. These trypanosomes were readily cultured in diphasic blood-agar medium.Cultures of T . hedricki n.sp. and T . myoti n.sp. were infective when inoculated orally or injected intraperitoneally into laboratory reared E. fuscus and M. lucifugus respectively. Pseudocysts of amastigotes were found in cardiac muscles of both bat species and in the intestinal smooth muscle of M. lucifugus. Trypanosoma hedricki n.sp. was not infective to M. lucifugus nor was T . myoti n.sp. infective to E. fuscus. Unlike T. cruzi, cultures were not infective to Mus musculus, Peromyscus maniculatus, Microtus pennsylvanicus, Mesocricetus auratus, Rattus norvegicus, and Cavia porcellus.After in vitro incubation in fresh plasma from deer mice, hamsters, laboratory rats, guinea pigs, little brown bats, and Homo sapiens, T . hedricki n.sp. could not be cultured. However, positive cultures were obtained after incubation in fresh plasma from E. fuscus and occasionally in fresh plasma from laboratory mice. Positive cultures were always obtained when the plasma was heat inactivated. BOWER, S. M., et P. T. K. Woo. 1981. Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario. Can. J. Zool. 59: 530-545. De novembre 1976 B mai 1979, le sang de 529 chauves-souris de 12 localitts difftrentes du sud de ]'Ontario a Ctt soumis ? i la technique de centrifugation afin d'y rechercher la presence de trypanosomes. Trypanosoma hedricki n.sp. a Ctt trouvt chez 62 des 216 Eptesicus fuscus examints et Trypanosoma myoti n.sp., chez 16 des 313 Myotis lucifugus. La phase sanguine de ces deux esp&ces est semblable morphologiquement B celle de Trypanosoma cruzi. Ces trypanosomes peuvent facilement &tre gardCs en culture en milieu diphasique sang-agar. Les cultures de T . hedricki n. sp. sont infectieuses lorsqu'elles sont administdes oralement ou par injection intra-abdorninale h des E. fuscus Clevts en laboratoire; de la m6me f a~o n , les cultures de T . myoti sont infectieuses pour des M. lucifugus. Des pseudocystes d'amastigotes se retrouvent dans le muscle cardiaque des deux espbces de chauves-souris et dans le muscle intestinal lisse de M. lucijiugus. Trypanosoma hedricki n.sp. ne parasite pas M, luclfugus et T . myoti n'est pas infectieux chez E. fuscus. Alors que T . cruzi est infectieux chez Mus musculus, Peromyscus maniculatus, Microtus pennsylvanicus, Mesocricetus auratus, Rattus norvegicus et Cavia porcellus, les cultures de T . hedricki et de T . myoti ne le sont pas. Des T. hedricki n.sp. ont t t t rnis en incubation in vitro dans du plasma ...

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Comparative drug sensitivities of culture forms of Trypanosoma cruzi and Trypanosoma dionisii

Cited by 7 publications

References 4 publications

Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae)

Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae)

Trypanosoma dionisii as an experimental model to study anti-Trypanosoma cruzi drugs: A comparative analysis with benznidazole, posaconazole and amiodarone

Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

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